To get ahead in your research you may want to image the smallest structures, catch the faintest signal or track the fastest processes – or do all of that at once. When it comes to getting accurate data from live cells or other weakly-labeled samples, there is no such thing as too much sensitivity, resolution or speed. Each photon of emission light is precious.
With Airyscan you collect all precious emission light without compromise and get a unique combination of gentle superresolution imaging and high speed. Use multicolor samples with any label and get image quality like you’ve never seen before. You get perfect optical sections and resolve structures of 120 nm (in x, y) and 350 nm (in z) even in thick samples. Plus, Airyscan gives you an instant 4 – 8× improvement of signal-to-noise (SNR).
You will also be harnessing this unique combination of greater SNR and
resolution at increased acquisition speeds of 27fps (at 480 x 480 pixels).
Comparing the confocal (left) with the Airyscan (right) image clearly shows improved resolution and signal-to-noise ratio. HeLa cells. Sample: Courtesy of S. Traikov, BIOTEC, TU Dresden, Germany
Imaging with a lateral resolution of 120 nm with a single image gives you more freedom to perform gentle live cell imaging experiments. mEmerald – TOMM20.
Sample: courtesy of M. W. Davidson, The Florida State University, Tallahassee, USA
LSM 880 Airyscan Fast; Acquisition speed: 96 fps.
Samples: Cardiomyocyte Cells with tubulin-EMTB to measure microtubule buckling. Courtesy of Ben Prosser, University of Pennsylvania, USA “Detyrosinated microtubules buckle and bear load in contracting cardiomyocytes”, Science April 2016.
Thanks to its excellent signal-to-noise ratio, Airyscan in Fast mode (video on the left) delivers more structural information when imaging highly dynamic processes.
Resonance Scanner using standard confocal; Acquisition speed: 80fps.
Samples: Cardiomyocyte Cells with tubulin-EMTB to measure microtubule buckling.
This information is clearly lost when using resonant scanning confocal microscopes (video on the right).
- Gain sensitivity, resolution and speed for all your experiments. Image with negligible phototoxicity or bleaching - without changing your workflow, sample labels or system operation.
- Airyscan’s unique Fast mode speeds up your imaging by a factor of four. This will propel you into the traditional domain of resonant scanning confocals without sacrificing sensitivity or resolution.
- Airyscan delivers a perfect optical section and superresolution with high sensitivity at 120 nm laterally and 350 nm axially. This transcends the deconvolution approach by preserving precious emission light normally rejected at a closed pinhole and achieving higher resolution.
Sample: U2OS Cells with Lifeactin; Max projection of 6 plane z-stack, 50 volumes over 17.5 minutes.
The patented Airyscan detector design allows gentle live cell imaging with superresolution. You collect more photons than with the closed pinhole of a traditional LSM and deconvolution.
- Combine the Fast mode for Airyscan with Z-stacks and tiling to image even large sample volumes with high image quality.
- Collect all your fluorescent signals in one go. Parallel acquisition lets you investigate multiple labels in minimum time, equipped with the highest number of detectors of any confocal.
- Improve your image quality with the unique combination of parallel spectral acquisition and high speed GPU deconvolution.
- Make the most of large fields of view and the highest speed of any linear scanning confocal – ZEISS LSM 880 with Airyscan delivers up to 27 fps at 480x480 pixels in Fast mode.
Drosophila embryo, depth coded maximum intensity projection. Airyscan in Fast mode. Courtesy of B. Erdi, Max F. Perutz Laboratories, University of Vienna, Austria
Use high frame rates in large fields of view with lowest light exposure for live imaging of whole organisms.
Combine the resolution and sensitivity advantage of Airyscan with multiphoton excitation for superior imaging deep in your specimens.
- Tailor Airyscan to your research needs with a choice of Superresolution, Sensitivity mode or the new Fast mode.
- Remove autofluorescence and simultaneously separate highly overlapping fluorophores in a single scan. This will minimize phototoxicity in the sample.
- Combine confocal imaging with single molecule techniques to gain mobility/concentration/oligomeric state information (FCS/FCCS/RICS/PCH).
- Select Airyscan’s Fast mode to perform functional multiphoton imaging deep inside your sample
Mitosis in HeLa-Kyoto cell line, imaged with ZEISS LSM 880 with Airyscan. Video showing Histone 2B (H2B, red, mCherry) and microtubule end binding protein 3 (EB3, green, EGFP) during mitosis.
Combine your data from multiple complementary imaging modalities – e.g., widefield, confocal and three different superresolution techniques. You can even correlate data with electron microcopy.
Gentle superresolution imaging allows long-term live cell imaging without artefacts caused by influencing the sample’s viability and biological function.
You capture more signal at high speeds while keeping light exposure to a minimum
Airyscan in Fast mode for Calcium Spark Imaging. With Airyscan in Fast superresolution mode you get more structural information at high speeds.
HeLa cells stained for Actin (green), Adapter Protein AP-3 (magenta) and Septin A (red). Courtesy of S. Traikov, BIOTEC, TU Dresden, Germany
Fixed tumor cells, tubulin labeled with Alexa 555, Airyscan SR mode. Sample courtesy of P. O'Toole and P. Pryor, University of York, UK.
Oligodendrocyte, CNPase-antibody staining. Courtesy of C. Dornblut, Leibniz Institute for Age Research (FLI), Jena, Germany
Drosophila embryo, maximum intensity projection. Microtubules labeled with GFP. Left: z-stack with 55 slices. Imaged for 203 min at 3 min interval. Courtesy of B. Erdi, Max F. Perutz Laboratories, University of Vienna, Austria
Drosophila embryo, maximum intensity projection. Microtubules labeled with GFP. Imaged at higher magnification. Z-stack with 117 slices, imaged for 75 min at 3 min interval. Courtesy of B. Erdi, Max F. Perutz Laboratories, University of Vienna, Austria
ZEISS LSM 880 with Airyscan
Your New Standard for Fast and Gentle Confocal Imaging
Filesize: 16,809 kB
The Basic Principle of Airyscanning
Filesize: 1,473 kB
White Paper: Airyscanning - Short version
A Novel Approach to Confocal Imaging
Filesize: 1,709 kB
Beampath - ZEISS LSM 880 with Airyscan
Revolutionize Your Confocal Imaging
Filesize: 607 kB
Application Note: Spectral Imaging: a Powerful Tool for Confocal Multicolor Imaging in Living Plant Cells
Filesize: 1,138 kB
Application Note: Cryo-Confocal Imaging with Airyscan
Improving Resolution and Signal-to-Noise in Cryo-Fluorescence Microscopy
Filesize: 1,483 kB
Technology Note: The Airyscan Detector from ZEISS
Confocal Imaging with Improved Signal-to-Noise Ratio and Superresolution
Filesize: 3,958 kB
Technology Note: ZEISS LSM 880 with Airyscan
Introducing the Fast Acquisition Mode
Filesize: 1,071 kB
Get in contact with us for more information on attractive new LSM 8 packages, to get your quote, or to schedule a product demonstration. Profit from more sensitivity, resolution and speed. Get the unique combination of gentle superresolution imaging and high speed with ZEISS LSM 8 confocal microscopes:
- Profit from our novel detector design: Airyscan gives you an instant 4 – 8× improvement of signal-to-noise
- See the smallest details: Resolve structures of 120 nm (in x,y) and 350 nm (in z)
- Track the fastest processes: Acquisition speeds of 27 fps (at 480x480, LSM 880 in Fast mode)
- Protect your sample and save time: Acquire the entire fluorescent spectra of all your labels at once