ZEISS Correlative Study for Yeast Research

ZEISS Correlative Study for Yeast Research

Better Understand Discrete Subpopulations of the Tagged Protein

Highlights

  • Enhance your understanding of the processes that regulate the receptors
  • Determine the intracellular localization of the receptors
  • Localize and visualize the receptors with high resolution
  • Relocate your sample with ease and speed by means of correlative coverslips

To enhance the understanding of processes that regulate the heterologous expression of a family of human adenosine receptors, researches applied ZEISS correlative workflows by taking advantage of  the benefits of Shuttle & Find software, correlative coverslips and overlaying of SR- and SEM datasets in ZEN. This approach allowed them to detect an electron-dense cisternal compartments which can be co-localized with the G-bound protein receptor labeled structures. A similar approach can be combined with other labeling/contrasting protocols and readily adapted to existing sample specific methods.

Yeast Cell Walls (SIM)

Yeast Cell Walls (SIM)

Courtesy of Jeffrey L. Caplan and Kirk J. Czymmek, Bioimaging Center, Delaware Biotechnology Institute.

High Magnification of Yeast Correlative Superresolution/FE-SEM

Courtesy: Jeffrey L. Caplan and Kirk J. Czymmek, Bioimaging Center, Delaware Biotechnology Institute.

Superresolution Yeast ELYRA PS.1 / AURIGA

Yeast PALM Overlay Compiled LowRes - Courtesy of Jeffrey L. Caplan and Kirk J. Czymmek, Bioimaging Center, Delaware Biotechnology Institute.

Yeast PALM Overlay Compiled LowRes

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