Combine imaging and analytical performance of a high resolution field emission scanning electron microscope (FE-SEM) with the processing ability of a next-generation focused ion beam (FIB). You may be working in a multi-user facility, or an academic or industrial lab. Take advantage of ZEISS Crossbeam’s modular platform concept and upgrade your system with growing needs, e.g. with the LaserFIB for massive material ablation. During milling, imaging or when performing 3D analytics Crossbeam will speed up your FIB applications.
- Maximize Your SEM Insights
- Increase Your FIB Sample Throughput
- Experience Best 3D Resolution in Your FIB-SEM Analysis
- Take advantage of up to 30% better SEM resolution at low voltage using Tandem decel, a feature of the novel ZEISS Gemini electron optics.
- Extract true sample information from your high resolution SEM images using Gemini electron optics.
- Count on the SEM performance of your ZEISS Crossbeam for 2D surface sensitive images or when performing 3D tomography.
- Benefit from high resolution, contrast and signal-to-noise ratios, even when using very low acceleration voltages.
- Characterize your sample comprehensively with a range of detectors. Get pure materials contrast with the unique Inlens EsB detector.
- Investigate non-conductive specimens undisturbed by charging artifacts.
- Benefit from speed and precision of intelligent FIB scanning strategies for material removal and perform your experiments up to 40% faster than before.
- The Ion-sculptor FIB column introduces a new way of FIB-processing: by minimizing sample damage you’ll maximize sample quality and perform experiments faster at the same time.
- Manipulate your samples precisely and fast by using up to 100 nA current without compromising FIB resolution.
- When preparing TEM samples use the low voltage capabilities of the Ion-sculptor FIB: get ultra-thin samples while keeping amorphization damage at a minimum.
- Enjoy the benefits of integrated 3D analysis for EDS and EBSD investigations.
- During milling, imaging or when performing 3D analytics Crossbeam will speed up your FIB applications.
- Expand the capacity of your Crossbeam with ZEISS Atlas 5, the market-leading package for fast, precise tomography.
- Perform EDS and EBSD analysis during tomography runs with the integrated 3D Analytics module of ZEISS Atlas 5.
- Gain unique insights from best 3D resolution and leading isotropic voxel size in FIB-SEM tomography. Probe less than 3 nm in depth and produce surface sensitive, material contrast images using the Inlens EsB detector.
- Save time by collecting your serial section images while milling. Achieve accuracy and consistency using trackable voxel sizes and automated routines for active control of image quality.
Leverage low vacuum operation and perform in situ experiments with outgassing or charging samples using the Variable Pressure mode. Achieve high quality imaging and high throughput enabled by the unique Gemini electron optics and the Ion-sculptor FIB.
Perform your most demanding characterizations and choose the chamber size, standard or large, that best suits your samples. The Gemini 2 electron optics enables high resolution, even at low voltage and high current. It’s ideal for high resolution imaging at high beam current and for fast analytics.
Your instrument for massive material ablation and preparation of large samples - the femtosecond laser on the airlock enhances in situ studies, avoids chamber contamination and is configurable as Crossbeam 350 and 550. Gain rapid access to deeply buried structures or prepare extremely demanding structures e.g. atom probe samples.
This solution for TEM lamella preparation and volume imaging under cryogenic conditions enables imaging near-to-native state. Connect widefield, laser scanning, and focused ion beam scanning electron microscopy. Keep the flexibility of a multi-purpose FIB-SEM simultaneously.
Rapidly access buried regions of interest, execute correlated workflows across multiple length scales, acquire better sample representativity with large-volume analysis, and perform 3D imaging and analytics. Add a femtosecond laser to your ZEISS Crossbeam and benefit from site-specific, ultra-fast sample preparation.
- Gain rapid access to deeply buried structures
- Prepare extremely large cross-sections up to millimeters in width and depth
- Benefit from minimal damage and heat affected zones due to femtosecond laser pulses in a controlled vacuum environment
- Perform laser work in a dedicated integrated chamber to maintain cleanliness of your FIB-SEM main chamber and detectors
- Find your deeply buried regions of interest (ROIs) by correlation with previously acquired 3D X-ray microscopy datasets or other external data
- Load your sample onto the holder and transfer into the FIB-SEM main chamber
- Import, overlay and align e.g. 3D X-ray data or 2D optical microscope images in ZEISS correlative workspaces
- Find your ROI and acquire a reference image
- Scan the four sample holder fiducials with the SEM to lock sample and SEM coordinates
- Transfer sample to integrated femtosecond (fs) laser chamber
- Scan the four sample holder fiducials with the fs laser to lock sample and laser coordinates
- SEM and laser coordinates are now aligned
- Draw your laser pattern
- Expose the laser pattern
- Quickly remove massive material volumes with better than 2µm targeting accuracy
- Details of the microstructures can already be observed
- Perform FIB polishing as required for high-resolution imaging
- Create TEM and atom probe samples with novel workflows
- Rapidly optimize laser recipes with immediate SEM feedback
TEM lamella preparation is essential for almost any FIB-SEM user. ZEISS offers an automated workflow for site-specific preparation. The resulting lamellae are ideally suited for high resolution TEM and STEM imaging and analysis at atomic resolution. Navigate to the specimen’s ROI, extract your TEM lamella including ROI from your bulk sample, perform the bulk milling or trenching step, and finalize the workflow with lift-out and thinning where appropriate.
- Begin the workflow without time-consuming search for the ROI
- Use the navigation camera on the airlock to locate specimens
- The integrated user interface makes it easy to navigate to your ROI
- Benefit from the large, distortion-free field of view in the SEM
- Start the preparation with a simple three-step process: ASP
- Define the recipe including drift correction, deposition and coarse and fine milling
- The ion optics of the FIB column enables high throughput for the workflow
- Duplicate the recipe and repeat as often as required in order to start a batch preparation
- Bring in the micromanipulator and attach the lamella to its tip
- Cut out the lamella from the bulk
- The lamella is then ready for lift out and can be transferred to a TEM grid
- The instrument’s design allows you to reach a desired thickness of the lamella by enabling live monitoring of the thinning
- Use two detector signals in parallel to judge lamella thickness and obtain reproducible end thickness on the one hand (with the SE detector) and to control surface quality on the other hand (with the Inlens SE detector)
- Prepare high quality samples with negligible amorphization
Cryogenic microscopy allows the examination of cellular structures in their near-to-native state. However, users face complex challenges, such as preparation, devitrification, ice contamination, loss of samples or correlation across imaging modalities. ZEISS Correlative Cryo Workflow connects widefield, laser scanning, and focused ion beam scanning electron microscopy in a seamless and easy-to-use procedure. Hardware and software are optimized for the needs of correlative cryogenic workflows, from localization of fluorescent macromolecules to high-contrast volume imaging and on-grid lamella thinning for cryo electron tomography.
- Seamless cryogenic workflow across multiple modalities
- Sample protection against devitrification and ice contamination
- High-resolution fluorescence imaging
- High-contrast volume imaging and 3D reconstruction
- Targeted on-grid lamella thinning for cryo TEM applications
- Multipurpose use for cryogenic and room temperature applications
With ZEISS Correlative Cryo Workflow, you master the challenging combination of different imaging modalities under cryo conditions. The workflow solution connects light and electron microscopy, enabling volume imaging and efficient production of TEM lamellae. Dedicated accessories simplify the workflow and facilitate a safe transfer of cryo samples between the microscopes. Data management is assured by ZEN Connect, which keeps your data in context throughout the workflow. A series of processing tools help you enhance the imaging results.
Thanks to cryo-compatible objectives and the high sensitivity of the Airyscan detector, ZEISS LSM systems enable you to detect proteins and cellular structures at high resolution while gentle illumination and constant low temperatures prevent your samples from devitrification. The ZEISS Crossbeam FIB-SEM lets you enjoy high-contrast volumetric imaging – even without heavy metal staining applied to your samples. Both modalities provide valuable functional and structural information that can give you a thorough understanding of ultrastructure, whether or not you follow up with TEM studies.
Unlike other solutions, the ZEISS microscopes involved in the workflow can be used not only for cryogenic microscopy, but also for room temperature applications, which is particularly advantageous when the microscopes are not being fully utilized for cryogenic experiments. Converting the instruments from cryogenic to room temperature usage is done quickly and doesn’t require technical expertise. This flexibility gives users more time for their experiments. Imaging facilities benefit from better utilization and a faster return on investment.
SEM Electron Optics
The FE-SEM column of ZEISS Crossbeams is based on Gemini electron optics as all ZEISS FE-SEMs. Decide on the Gemini VP column of Crossbeam 350 or the Gemini II column of Crossbeam 550.
Field emission SEMs are designed for high resolution imaging. Key to the performance of a field emission SEM is its electron optical column. Gemini technology comes with all ZEISS FE-SEMs and FIB-SEMs: it is tailored for excellent resolution on any sample, especially at low accelerating voltages, for complete and efficient detection, and ease-of-use.
Gemini Optics is Characterized by Three Main Components
- The Gemini objective lens design combines electrostatic and magnetic fields to maximize optical performance while reducing field influences at the sample to a minimum. This enables excellent imaging, even on challenging samples such as magnetic materials.
- Gemini beam booster technology, an integrated beam deceleration, guarantees small probe sizes and high signal-to-noise ratios.
- The Gemini Inlens detection concept ensures efficient signal detection by detecting secondary (SE) and backscattered (BSE) electrons in parallel minimizing time-to-image.
Benefits for Your FIB-SEM Applications
- Long-term stability of the SEM alignment and the effortless way it adjusts all system parameters such as probe current and acceleration voltage
- Achieve distortion-free, high resolution imaging even over large fields of view with the help of the near magnetic-field free optics
- Tilt the specimen without influencing the electron optical performance
- Maximum sample flexibility in multi-purpose environments
- In situ experiments with outgassing or charging samples
- Unique Gemini material contrast with the Inlens EsB detector
- High resolution even at low voltage and high current thanks to the double condenser system
- More information in less time with high resolution imaging and fast analytics
- Unique topographical and material contrast with simultaneous Inlens SE and EsB imaging
Gemini Novel Optics
High resolution imaging at low landing energy is required for beam as a standard. It is essential for:
- beam sensitive samples
- non-conductive materials
- gaining true sample surface information without undesirable background signal from deeper sample layers
The novel Gemini optics are optimized for resolutions at low and very low voltages and for contrast enhancement.
Technological characteristics are the high gun resolution mode and the optional Tandem decel.
- The high gun resolution mode results in minimized chromatic aberration thanks to of a reduction of primary energy width by 30%.
Tandem decel, now introduced to ZEISS Crossbeam 350/550, can be used in two different modes:
- Tandem decel, a two-step deceleration mode, combines the beam booster technology with a high negative bias voltage that is applied to the sample: the electrons of the primary electron beam are decelerated, thus the landing energy is effectively reduced
- Apply a variable negative bias voltage between 50 V and 100 V. One application mode enhances the contrast of your images
- Apply a negative bias voltage between 1 kV and 5 kV and improve the low kV resolution of your images
The Ion-sculptor FIB column speeds up your FIB work without compromising machining precision and lets you benefit of its low voltage performance for any sample.
The ZEISS Crossbeam Family carries the next-generation focused ion beam column, Ion-sculptor, featuring high currents for high throughput and excellent low voltage performance for high sample quality.
- Maximize sample quality by using the low voltage capabilities of the Ion-sculptor FIB column
- Minimize amorphization of your specimens and get the best results after thinning
- Get precise and reproducible results with maximum stability
- Accelerate your FIB applications with fast probe current exchanges
- Perform high throughput experiments thanks to beam currents of up to 100 nA
- Achieve exceptional FIB resolution of less than 3 nm
- The Crossbeam family comes with automatic FIB emission recovery for long-term experiment
Live imaging of FIB-milling a spiral in silicon. Imaged with the SEM using an Inlens detector.
Lithium ion battery example, product key featuring cross-sectioning & 3D Tomography and 3D Analysis LiMn2O4 cathode material of a lithium ion battery. Close-up of cross-section shows surface information on an Inlens SE image A). The distribution of lanthanum (red) and manganese (green) is derived from an EDS map B).
H-bar lamella preparation by fs-laser on a copper semi-circle grid. The left lamella is 400 μm wide, 215 μm deep and has a thickness of about 20 μm at the top. It was cut by the laser in 34 s. The amount of material that needs to be removed by FIB for final thinning is significantly reduced.
Key feature fs laser machining: Array of compression testing pillars in high entropy alloy, machined fully automatically.
Deep laser cut in electronics sample to gain access to buried ROI in 860 µm depth.
3D-NAND – FIB-SEM Tomography
FIB-SEM tomography dataset acquired from a commercially purchased 3D NAND sample. Sample was depackaged and mechanically polished down to the topmost word line. Data acquisition was done on ZEISS Crossbeam 550 using ZEISS Atlas 3D. Voxel size 4 x 4 x 4 nm3.
Left: 3D rendering of the complete 2 x 4 x 1.5 µm3 volume.
Middle: Virtual sub-volume of 2 x 1.5 x 0.7 µm3 size, extracted from the dataset at the transition region of upper to lower deck.
Right: Single horizontal slice taken from the volume, showing a top-down view of a word line.
Power Electronics – Insulated Gate Bipolar Transistor
Insulated Gate Bipolar Transistor (IGBT) device analysis. The analysis was performed entirely on ZEISS Crossbeam 550.
First, a FIB cross-section across the gate electrode was cut, exposing irregularly distributed dark features (top).
Second, a lamella was prepared from the left-hand part of that cross section, and imaged by 30 kV STEM-in-SEM. The brightfield STEM image shown here reveals that the features are crystalline precipitates (bottom).
Third, EDS elemental mapping of the lamella showed that the precipitates are silicon (right).
3D Stacked Die Interconnect
Crossbeam laser provides fast, high-quality cross sections of Cu-pillar microbumps buried 760 µm deep with total time to results of <1 hour.
Left: 3D integrated circuit (IC) flip chip prepared for microbump imaging with laser ablation and FIB polishing.
Right: 25 µm diameter microbump image acquired with backscattered electrons.
Cell Biology – HeLa Cells
Investigation of different cell compartments in single cells.
Individual HeLa cells were grown in culture dishes, chemically fixed
and resin-embedded in EPON. Voxel size 5 × 5 × 8 nm,
Inlens EsB detection, 1400 sections. 3D visualization with
Dragonfly Pro, ORS. Courtesy: A. Steyer and Y. Schwab, EMBL,
Developmental Biology – C. elegans
Understanding the morphology of a whole organism in 3D with the highest resolution and reliability. The data set shows a arge 3D volume of C.elegans consisting of 10.080 z-sections at 5 x 5 x 8 nm pixel size. The nematode was high pressure frozen and freeze-substituted in EPON. Even the smallest structures inside the worm can be identified very easily.
Courtesy: A. Steyer and Y. Schwab, EMBL Heidelberg, DE; and S. Markert and C. Stigloher, University of Wuerzburg, DE.
Neuroscience – Brain Sections
Large area milling and imaging of a brain section with the
3D module of ZEISS Atlas 5. High current allows fast milling and
imaging of large fields of view up to 150 μm in width. The depicted
brain image has a field of view of 75 μm in width and
was milled with a beam current of 20 nA. Courtesy: C. Genoud,
FMI Basel, CH.
Microbiology - Trypanosoma
Ultrastructural investigation of the parasite Trypanosoma brucei.
The cells are high pressure frozen and freeze-substituted in EPON. Acquisition of 800 z-sections which corresponds to ~ 8 μm thickness in z; pixel size in x/y is 5 nm. Sample courtesy: S. Vaughan, Oxford Brookes University, Research Group ’Cell biology of Trypanosomes’, UK.
ZEISS recommends Dragonfly Pro from Object Research Systems (ORS)
An advanced analysis and visualization software solution for your 3D data acquired by a variety of technologies including X-ray, FIB-SEM, SEM and helium ion microscopy.
Available exclusively through ZEISS, ORS Dragonfly Pro offers an intuitive, complete, and customizable toolkit for visualization and analysis of large 3D grayscale data. Dragonfly Pro allows for navigation, annotation, creation of media files, including video production, of your 3D data. Perform image processing, segmentation, and object analysis to quantify your results.
Add the ToF-SIMS (time of flight secondary ion mass spectrometry) spectrometer to your Crossbeam 350 or Crossbeam 550 and analyze trace elements, light elements (e.g. lithium), and isotopes. Profit from sensitive and comprehensive analyses in 3D. Perform elemental mapping and depth profiling. Benefit from parallel detection of atomic and molecular ions down to the ppm level, achieve resolutions better than 35 nm in lateral direction and 20 nm in depth. Retrieve any signal from the ROI post-mortem.
ZEISS Crossbeam Family
Your FIB-SEM for High Throughput 3D Analysis and Sample Preparation
file size: 7777 kB
ZEISS Crossbeam Family
Introducing ToF-SIMS enables High Throughput in 3D Analysis
file size: 1427 kB
ZEISS ORS Dragonfly
Outstanding 3D visualization with best-in-class graphics
file size: 689 kB
Technology Note: ZEISS Crossbeam
Reproducible TEM Lamella Thinning by FIB with Real-time Thickness Control and End-point Detection
file size: 1394 kB
Technology Note: ZEISS Crossbeam 550
High Throughput Imaging
file size: 2044 kB
FIB-SEM Investigations of the Microstructure of CIGS Solar Cells
file size: 1388 kB
High Resolution STEM and EDS Study of Chromium Depletion in Stainless Steel
file size: 1614 kB
Application Note: FIB-SEMs
X² STEM Lamella Preparation from Multicomposite Organic Electronic Devices with ZEISS FIB-SEMs
file size: 883 kB
ZEISS Microscopy Solutions for Steel and Other Metals
Multi-modal characterization and advanced analysis options for industry and research
file size: 15285 kB
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