Cell Observer

Efficient observation of living cells

With the versatile Cell Observer system, you visualize living organisms and intracellular processes, even over a period of several days. Acquire time lapse and Z-stack images in a fraction of a second with the Cell Observer HS, high-speed version. It delivers up to 300 images per second at equidistant intervals and with reproducible acquisition conditions.

Dictyostelium cells
Gene expression
HeLa cells

Highlights

Complete system for rapid acquisition of images in several dimensions
Combine up to 32** or 5* channels, Z-stacks, time lapse images, and any number of specimen positions and tiled** images
Optional high-speed components, such as fast-switching, excitation light sources, shutters and piezo steppers*
Time lapse images with up to 85 images/s in 2-channel fluorescence or up to 300 images/s in single-channel mode*
Second-by-second acquisition of a Z-stack image in 2-channels for 3D information*
Incubators configurable with heat, carbon dioxide or oxygen controls
Thermally stabilized chamber incubator for experiments over long periods
Equidistant intervals ensured by separating image processing from acquisition*

*) Cell Observer HS
**) Cell Observer

Versatile & Fast

Convenient generation of meaningful time lapse images

With Cell Observer, you can structure your experiments with extreme flexibility. The system has various incubators which guarantee constant culture conditions for the entire duration of your experiment. In addition, with Incubator XL, the system obtains the thermal stability essential for long term experiments.

Acquire, process and measure dynamic processes in a wide variety of applications and publish them as attractive video sequences. All this is possible, with a very low learning curve, due to the universal operating concept and optimally integrated system components.

Capture events in several dimensions at very high acquisition speeds. The high-speed Cell Observer HS enables images acquisition of rapid changes to cells and cell structures in several dimensions and with free selection of the right contrasting technique.

Equipped for your biological applications

Time-critical components, such as adjustable light sources or piezo z-drives, are controlled independently from the operating system and therefore can react with very short response times. The multidimensional image is put together once the raw data for the complete image series have been stored on the hard drive. This saves time for acquisition, as the computing intensive processing steps are made after the experiment.
Therefore, with Cell Observer HS, constant intervals are achieved between the single images of each time point as well as at rapid acquisition speeds.

Definite Focus.2

A Feature Designed to Eliminate Z Drift, thus Ensuring Maximum Reliability in Live Cell Imaging

Stay on track - stay focused. Definite Focus.2 uses a projected grid image to measure the change in path length between the objective and the bottom of the culture vessel. On demand Definite Focus.2 corrects thermally caused focus drifts as well as Z-Offsets introduced when moving to new sample positions. via the Z-Control of the Axio Observer or an additional high-speed Z-Piezo.

You can start experiments without waiting for the complete microscope to reach thermal stability. Unrestricted fluorescence microscopy. The wavelength of the LED is 850 nm – way out of the excitation and emission spectra of all common fluorochromes.

Cruise your sample and let Definite Focus.2 do the job to update the focus on the fly. Once activated neither tilted nor skewed culture vessels will stop it, not even changing from well to well of the same plate.

Definite Focus permits dynamic temperature experiments. The desired temperature jumps are no problem, as Definite Focus will immediately compensate the drift.

Unimpaired flexibility. Definite Focus is compatible with all customary objectives and contrast methods. Use dishes with plastic or glass bottoms, as your application requires.

Use the unique operating concept of Axio Observer.Z1: The central TFT display lets you control or watch Definite Focus just like all other microscope components.

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Cell Observer - Living Cells

Observe and Analyze: The Systems Approach for Imaging Living Cells and Organisms

Pages: 28
Filesize: 4,004 kB

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