ZEISS PALM CombiSystem

Two Non-Contact Laser Technologies in One System

PALM CombiSystem

Laser Microdissection and Optical Tweezers in a Single System

PALM CombiSystem combines laser microdissection with optical trapping. For laser microdissection mark an element on your screen and then start the laser to separate it from unwanted tissue. A targeted laser pulse will 'catapult' your element upwards and contact-free into a collection vessel for further analysis.

The optical trap of PALM CombiSystem manipulates particles with the highest precision at the cellular and sub-cellular level. This allows you to trap, move and position microscopic objects – such as red blood cells and bacteria – intuitively with the force of light.

PALM CombiSystem
Micromanipulations Made Accurate and Simple


Extend Your System to a Complete Research Platform

PALM CombiSystem offers you exceptional control over laser micromanipulation

  • Microdissect samples from fixed material to living cells for DNA, RNA and protein isolation
  • Applicable to cryosections, FFPE (formalin fixed and paraffin embedded) tissue sections, cell smears, cytospins, chromosome preparations and live cells
  • Trap, sort and position with the highest precision
  • Manipulate and move particles and living cells in liquids
  • Connect modular equipment, such as AxioCam high-resolution digital cameras for fluorescence and bright field that brings additional clarity to your work
  • Enjoy an intuitive user interface and functions that are easily incorporated into your workflow
  • Benefit from the expertise and technical support of the ZEISS specialists

PALM CombiSystem by ZEISS is a platform that can be integrated with future-oriented technologies – ensuring many years of flexible service in your lab.

 PALM CombiSystem is supplied together with the Axio Observer inverse research microscope
PALM CombiSystem is supplied together with the Axio Observer inverse research microscope


PALM CombiSystem is Integrated Into the System World of ZEISS

PALM CombiSystem is supplied together with the Axio Observer inverse research microscope. That means you not only benefit from an optical trap and microdissection capabilities, but you also enjoy exceptional, proven ZEISS imaging quality.

In addition, your PALM CombiSystem is compatible with many other ZEISS components, allowing you to extend your system and create the versatile research platform you demand with a maximum return on your investment.


Contact-Free High Precision Micro-Manipulation

PALM CombiSystem uses a focused laser beam to cut out and isolate your selected specimen without contact. The patented laser catapult isolates your target area fast and uncontaminated.

In addition, PALM CombiSystem provides optical tweezers. These are the ideal solution if you demand a high-precision tool for the separation and manipulation of particles at the cellular and sub-cellular level.


The PALM Family - A New Dimension in Sample Purity

Integrated Laser Microdissection and Microscope Systems for Live Cells and Fixed Material

pages: 23
file size: 9523 kB

Laser Microdissection: A Key Technology for Microproteomics

Isolate a single cell population Isolate 30.000 cells in one step within seconds Seamless integrate LCM into your workflow

pages: 7
file size: 2197 kB

Results 1 - 2 of 2


Including MicroBeam, CombiSystem & MicroTweezers

Protocols of ZEISS Labs

The Unique Microdissection Service

LCM Protocols – Protein Handling for LC/MS
Non contact Laser Capture Microdissection - June 2011
(24 pages)
Download (7.630 kB)

PALM Protocols – RNA handling
Dos and Don’ts, slide preparation, archived samples, staining procedures, storage, laser microdissection procedure, collection devices and procedures. Downstream applications, RNA from FFPE and frozen sections, QIAGEN RNeasy FFPE kit, quality control, lab tips.
(28 pages)
Download (612 kB)

PALM Protocols – DNA handling
Preparation and treatment of slides, mounting samples onto slides, staining procedures, laser microdissection procedure, collection devices and procedures, cap check. Downstream applications, DNA from FFPE and frozen sections. QIAamp DNA procedure. PCR set up.
(26 pages)
Download (8.017 kB)

Chromosome Preparation
Chromosome preparation, staining, procedure of laser microdissection, collection procedures, collection onto AmpliGrid slide, consumables and components. Collection devices, lenses.
(20 pages)
Download (7.147 kB)

Cell Culture, non-contact Laser Capture Microdissection (LCM) and Recultivation
Cultivation of cells, coating of Dishes and Slides, improvement of visualization, procedure of laser microdissection, recultivation, time lapse, incubators. Optical Tweezers.
(14 pages)
Download (500 kB)

Immunofluorescence on frozen sections
Tips before starting, microtome cutting, antibody staining.
(5 pages)
Download (437 kB)

FISH Hybridization
Chromosome preparation. Preparation of chromosome paints. Hybridization of painting probes. Preparation of centromeric probes.
(15 pages)
Download (389 kB)

AutoLPC from glass slides results in good quality RNA
For RNA experiments on microdissected samples we usually recommend the use of MembraneSlides. Sometimes only old archived slides are available. We show that even this kind of slides is useful for RNA preparation. After removing the coverslip the unique and proprietary software function of “AutoLPC” allows quick access to the desired tissue area. And we could prove that this method does not influence the RNA quality.
(6 pages)
Download (2.287 kB)

RNA extraction from FFPE sections
Short protocol. Tips and recommendations for using FFPE sections as source for RNA extraction.
(4 pages)
Download (568 kB)

RNA extraction from frozen sections
Short protocol. Especially helpful for RNase rich tissue. Tips for microtome cutting and special staining (cresylviolet).
(4 pages)
Download (179 kB)