ZEISS ZEN Image Processing
ZEISS ZEN
Image Processing
Image Processing

ZEISS ZEN for Image Processing

Colocalisation

The Colocalisation module can be used to unmix the overlapping signals of different fluorescence channels.
To do this, the ZEN software makes use of modern methods, such as the automated threshold value method of Costes et al. Meaningful results on the colocalization of proteins can be obtained as a scatter plot, image and data table

If you only want to analyze certain areas of the image, you can simply mark the corresponding region directly in the image and scatter plot. Spectral Colocalisation for ZEN then supplies the data both for the whole image and the selected regions.
Of course you can also export your measured values in CSV format and process them further using a spreadsheet.

Deconvolution

Due to the optical properties of the microscope system, light from the areas above and below the focal plane also enters the image. This means that fine structures on the specimen can no longer be picked out.

It is precisely here that the 3D Deconvolution software module starts, by calculating out of focus light back to its place of origin. The distorted specimen structure unfolds and is displayed more sharply. This technique also reduces any image noise that may be present, making a decisive contribution to the first-class image quality of your image stacks and 3D models.

3Dxl

With 3Dxl you can display multi-dimensional image stacks in 3D. The ZEN module processes up to 8 different channels and works seamless with other image dimensions such as time (ā€œ4Dā€) or multiple tiles. Highlight is its ability to handle really large images (hundreds of gigabyte) efficiently and smoothly.

You can position a three-dimensional model interactively according to the view that you require and provide it with annotations, coordinates and scaled axes. It goes without saying that 3Dxl also provides you with tools for the interactive measurement of distances and angles. Would you like to present or publish the result afterwards? If so, simply export the images and animations into common image formats, such as TIF, JPG or AVI.