ELYRA Superresolution Microscopy
For Structured Illumination and Photoactivated Localization Microscopy of small structures

ELYRA Superresolution Microscopy

Your Flexible Imaging System for 3D Superresolution Microscopy

ELYRA superresolution microscopes are about choice: with ELYRA S.1 and superresolution structured illumination (SR-SIM) you image fine structural details while remaining free to label your samples with conventional dyes.

Use ELYRA P.1 and superresolution photoactivated localization microscopy (PALM) for endogenously-expressed photo-switchable fluorescent proteins.

Capture highly resolved structures of a whole cell in 3D in just one shot, while treating your sample so gently it stays fit for long-term observation. Your ELYRA system lets you reveal the ultrastructure of your object of interest, count molecules to quantify your results and see the arrangement of proteins within a structural context.

The choice is yours: The ELYRA product line puts the two most powerful and versatile superresolution technologies at your disposal. You can even combine them in one system with LSM 880. All that, plus ELYRA works seamlessly together with your ZEISS SEMs in a correlative workflow.

Photoactivation Localization Microscopy (PALM)

Photoactivated localization microscopy (PALM) is a superresolution technique that dramatically improves the spatial resolution of the optical microscope by at least an order of magnitude (featuring 10 to 20 nanometer resolution), which enables the investigation of biological processes at close to the molecular scale. The technique relies on the controlled activation and sampling of sparse subsets of photoconvertable fluorescent molecules, either synthetic or genetically-encoded. This interactive tutorial explores the sequential steps involved in creating a PALM image.

Using photoactivatable fluorescent proteins, it is possible to selectively switch on thousands of sparse subsets of molecules in a sequential manner. The basic principle behind PALM is to start with the vast majority of the molecules in the inactive state (in effect, not contributing fluorescence emission). A small fraction (less than 1 percent) is photoactivated or photoconverted using a brief pulse of ultraviolet or violet light to render that subset fluorescent. The activated molecules are then imaged and localized to produce nanometer-level precision coordinates, followed by removal from the larger set of unactivated molecules by photobleaching. In the next step, a second fraction of molecules is photoactivated, localized, and eliminated by photobleaching. The process is repeated many thousands of times until the molecular coordinates of all labeled molecules are obtained. The PALM image is a composite of all the single molecule coordinates. As new fluorescent probes for PALM are developed, the photoconversion and readout wavelengths are likely to ultimately span the entire ultraviolet, visible, and near-infrared spectral regions.

Contributing Authors

Adam M. Rainey, Tony B. Gines and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

Tutorial: Photoactivated Localization Technique

Discover the Nobel Prize in Chemistry 2014 Nature Collection, sponsored by ZEISS.




Count on Highest Reproducibility

  • Use motorized control of hardware components for easy switch between the fluorescence imaging modes widefield (WF), SR-SIM, PALM and laser scanning microscopy (LSM)
  • Acquire multicolor superresolution data with up to four channels using AOTF-controlled laser lines and a wide choice of filters
  • Profit from specialized and selected objectives and advanced EM-CCD and sCMOs camera detectors adapted to each superresolution technology, to achieve precise and reproducible result

Never Compromise on Image Quality

  • Specially-designed gratings let you select the best resolution for each wavelength
  • Choose the precise field-of-view (FOV) size to capture a whole cell or increase laser power densities for more efficient photo-switching
  • Render images in 3D using localization precision information
  • Profit from a range of illumination schemes (EPI, HILO, TIRF) and powerful algorithms for drift and color correction for highest image quality

Put Flexibility First

  • Choose between two powerful superresolution microscopy technologies: ELYRA S.1 for SR-SIM, ELYRA P.1 for PALM, or combine both in ELYRA PS.1
  • Integrate your ELYRA system with LSM 880
    Use ZEN Shuttle & Find for correlative microscopy and navigate between information from different imaging modes


Localize Single Molecules with Unrivalled Precision

Axio Lab.A1 – versatile applications

ELYRA P.1 takes light microscopy to the limit. By localizing small structures and even single molecules, you are able to achieve resolutions of down to 20 nm laterally and 50 nm axially.

You are interested in processes that take place near the coverslip. You want to see and measure single molecules in or near the plasma membrane like lipid rafts, receptor clustering or cell-substrate adhesion sites. With 3D-PALM you use photo-switchable proteins and profit from an excellent z capture range.

The patented exclusive PALM technology of ELYRA P.1 takes you into a new world of data quality. Detection with an effective resolution down to 20 nm will show you substructure and patterns where conventional light microscopy will simply show co-localization.

As a single molecule method, PALM is inherently quantitative – every image is a molecular statistics experiment.


Images any fluorophore – with up to twice the resolution of a conventional light microscope

Axio Lab.A1 – robust and long-term stable

You have invested a lot of time and energy in producing fusion proteins and multicolor staining protocols that are adapted to your experimental system. Now, with ELYRA S.1, you capture superresolution microscopy data with ease, using samples that may already be available in your lab's freezer. Specially-designed gratings give you the best resolution for each wavelength. Do you need Z-sectioning for 3D data acquisition? A fast, light-efficient detection? Then ELYRA S.1 is your ideal choice.


Your Flexible Imaging System for 3D Superresolution Microscopy

Forget about compromises.

ELYRA PS.1 lets you choose the superresolution method that’s best adapted to your specimen – at any time of an experiment – and with the superb image quality

Combine ELYRA PS.1 with LSM 880: never have so many imaging modalities been available on a single platform. The complexity of experiments in biomedical research often takes you beyond what a single imaging method can provide. Because you are interested in processes that take place within the context of an entire cell, superresolution imaging will work best if you can image a whole cell at the same time, switching between different imaging methods even in the course of an experiment.





Your Flexible Imaging System for 3D Superresolution Microscopy

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ZEISS ELYRA (in Portuguese Language)

Seu sistema de imagem flexível para microscopia de superresolução 3D

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ZEISS ELYRA (in Spanish Language)

El sistema de captura de imágenes flexible para microscopía de superresolución en 3D

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ZEISS ELYRA – a Quick Guide

Sample Preparation for Superresolution Microscopy

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ZEISS Elyra PS.1

Imaging Biological Samples – a Reference List

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