Cell signaling has inherent importance to extracellular perception but also underlies cell morphogenesis and tissue development. Confocal laser scanning microscopy allows us to define subcellular localization and relationships between signaling proteins, cytoskeletal elements and endomembrane compartments. The following ZEISS product is available to work on this topic: LSM 710. The system operates with Zeiss Zen 2012 (black version) software with built in tools for quantitative FRAP, FRET and co-localization.
- Microscopic phenotyping for plant developmental biology
- Characterization of plant cell form and tissue architecture
- Multicolor immunofluorescence localization of multiple antigens in various fixed plant samples (e.g. colocalization of signaling proteins with cytoskeleton)
- Live imaging of genetically encoded fluorescent protein fusions with different spectral properties

Arabidopsis primary root (left) and Arabidopsis lateral root primordium (right)
Knowhow/Expertise
- Fixed or living sample preparation and multicolor imaging
- Quantitative colocalization between different fluorophores (Pearson’s and Manders’ coefficients)
- Post-acquisition analysis (3-D volume rendering, fluorescence intensity profiling, orthogonal projection)
- High resolution live cell imaging
- 2-D time lapsed microscopy
- Quantitative analysis of fluorescence recovery after photobleaching
Quantitative CLSM applications in fixed and living samples
