• Overview

    Core Facility for Integrated Microscopy (CFIM)

    labs@location Partner

    Core Facility for Integrated Microscopy
    Panum Institute, building 21 floor 01
    Nørre Allé 20
    Faculty of Health and Medical Sciences
    University of Copenhagen
    DK-2200 Copenhagen N
    • Confocal Laser Scanning Microscopy
    • Spinning Disc
    • TIRF
    • Super Resolution Microscopy
    • Automated Slide Scanning
    • Laser Microdissection
    • Training and support for researchers

    Decide on your ideal ZEISS microscope in the facilities of our labs@location partner, the Core Facility for Integrated Microscopy (CFIM) at the University of Copenhagen.

    CFIM is a core facility in microscopy, located at the Faculty of Health and Medical Sciences, University of Copenhagen. CFIM welcomes all researchers of any level of microscopy experience from the University of Copenhagen, other universities and hospitals, and industry. Our vision is that CFIM allows users to think broadly and select the best possible instrument(s), to provide detailed answers to their scientific questions at the highest resolution. Our aims are to support and train scientists in the use of state of the art microscopes so that they get the most out of their experiments.

  • LSM

    Laser scanning confocal microscopy has become the classical imaging technique in biology, allowing for good resolution and a lot of flexibility in staining.
    CFIM offers three LSMs so that most samples can be accommodated.

    Microscope LSM 700 LSM 710 LSM 780
    Type Up-Right Up-Right Inverted
    Detectors 2 PMTs

    2 PMTs

    1 QUASAR

    2 PMTs

    1 GaAsp

    Live Imaging No No Yes;Temperature and CO2 control
    Excitation 405/488/555/639nm 405/458/488/514/561/633nm 405/458/488/514/543/633nm InTune laser from 490 to 625nm
    Contrast Techniques DIC DIC DIC
    Other options   Dipping Lens FRAP,FCS
  • Spinning Disc

    Spinning disc microscopy is ideal for fast confocal imaging. It is especially useful when imaging fast processes in live cells, for 3D overtime or recording big stacks.

  • Superresolution

    Elyra PS.1 is a superresolution microscope equipped to perform both Structured illumination microscopy and localization microscopy.


    Structured Illumination Microscopy (SIM) improves the resolution by a factor 2 (xy: 100nm; z: 250nm) relying on light interferences creating moire fringes.

     

    Localization microscopy techniques such as dSTORM and PALM take advantage of the blinking properties of some fluorophores to calculate their precise location in a sample. The resolution is greatly improved (xy: 40nm ; z: 100nm via TIRF).

  • Microdissection

    Microdissection allows one to select and isolate part of a sample and use it for further studies (RT-PCR, protein analysis, selection of live cells). The system is also paired with optical tweezer to manipulate samples.

  • Axio Scan

    Axio Scan is an automated slide scanner for brightfield and fluorescence microscopy. Up to 100 slides can be loaded and scanned with 10x/0.45, 20x/0.8 or 40x/0.95 objectives.

  • TIRF

    Both our Elyra and our Cell Observer (Spinning disc) microscopes are equipped with Total Internal Reflection Fluorescence microscopy (TIRF) modules.

    In TIRF microscopy laser light strikes the coverslip at a high angle. Most of the excitation light is reflected and only the 100 nm of the sample closest to the coverslip are excited. This leads to an enhanced signal to noise ratio for objects found in close proximity to the coverslip and a greatly enhanced z-resolution.

    WF vs TIRF

    Total Internal Reflection Fluorescence Microscopy

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