labs@location Partner, IFIB Tübingen - Germany
labs@location Partner
Interfaculty Institute of Biochemistry (IFIB)
Tübingen, Germany

Interfaculty Institute of Biochemistry (IFIB)

University Tübingen

Interfaculty Institute for Biochemistry
Signaltransduction – Transgene models
University Tuebingen
Hoppe-Seyler-Str. 4
72076 Tuebingen
Germany
  • Confocal and Widefield Microscopy
  • Live Cell Imaging
  • Microinjection
  • Ca2+ Imaging
  • FRET-based Imaging-Methods
  • FRAP
  • Photoactivation and Photoconversion
  • FCS, FCCS
  • TIRF Imaging
Section of mouse cerebellum expressing a cGMP sensor in Purkinje cells

Section of mouse cerebellum expressing a cGMP sensor in Purkinje cells

The Interfaculty Institute of Biochemistry (IFIB) is a vertically integrated research center covering all dimensions of modern biochemical research from atoms via molecules and cells to organisms. Fluorescence microscopy is central to the multidisciplinary research at IFIB, which spans scales from single molecules to cellular organelles to intravital imaging.


Intravital FRET-based imaging
We use a combination of state-of-the-art transgenic mouse and imaging technologies to "watch" biochemical processes in real time in living cells, tissues and mice. Of particular interest is the development and application of FRET-based sensor proteins for visualization of cell signaling via the cyclic nucleotide cGMP.

Single mRNA particles in yeast cells

Single mRNA particles in yeast cells

Imaging mRNAs in cells
RNA imaging can be used to follow the distribution of specific mRNAs inside a cell as well as for expression analysis (‘mRNA particle counting’). We use single molecule RNA fluorescent in-situ hybridisation (smFISH) and RNA-tagging methods to analyse the distribution of specific mRNAs inside cells, both at the single molecule and ensemble level. Currently, our specific focus is the question how certain mRNAs are targeted to the surface of intracellular organelles for their local translation.


Single molecule techniques for membrane biophysics
To investigate dynamic processes in biological membranes, we use model systems of different complexity, ranging from pure lipid bilayers to cultured cells. A common feature of these membrane systems is that they can be visualized with optical microscopy and, as a consequence, they can be used for time-lapse microscopy, FRAP, FRET, FCS and other advanced microscopy techniques.
Fluorescence cross-correlation spectroscopy can be used to detect and quantify molecular interactions. In the case of membrane lipids and proteins, we additionally have expertise in specialized methods like scanning FCS and FCCS, including two-focus measurements.
We also use single molecule imaging to study dynamics, oligomerization and complex formation of membrane components.

Nucleus and mitochondria of HeLa cell

Nucleus and mitochondria of HeLa cell

Know-how/Expertise:

  • Confocal (point-scanning, line-scanning, spinning disk) and widefield microscopy
  • Live cell imaging
  • Microinjection
  • Intravital imaging of mice
  • Ca2+ imaging (e.g., ratiometric)
  • FRET-based imaging methods (e.g., ratiometric, sensitized emission, acceptor photobleaching)
  • FRAP
  • Photoactivation and photoconversion
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence cross-correlation spectroscopy (FCCS)
  • Scanning FCS in membranes
  • TIRF imaging
  • Single particle tracking
  • Single molecule brightness-based stoichiometry analysis
  • Single molecule subunit photobleaching counting
     
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