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Oocyte Preparation and Quality

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Before the ICSI or IVF procedure you have to assess the quality of the oocytes, and select and prepare them for the following fertilization process.

Oocyte quality is assessed best via stereomicroscopic investigation of:

Oocyte cytoplasm abnormalities such as vacuolization and granularity

  • Zona Pellucida
  • Shape abnormalities
  • Oocyte “Sunburst Appearance” of the surrounding follicular cells

Prior fertilization the outer somatic cell layers (except the zona pellucida) surrounding the oocyte must be removed. The denuding is also done under stereomicroscopic control inside a petri dish. The oocytes are repeatedly inhaled into a denuding pipette until all outer cell layers have been sheared off. Thereafter the oocyte is examined with respect to morphological abnormalities. A constant temperature of approximately 35 - 37.5 °C has to be ensured throughout the whole process of oocyte collection, denudation, and injection. That’s why your dissection stereo microscope can be equipped with a heating stage and fit directly inside the laminar flow box.

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Oocyte preparation with stereo microscope for OVF, ICSI, IMSI, assisted reproductive technology
Oocyte preparation with stereo microscope for OVF, ICSI, IMSI, assisted reproductive technology
Oocyte preparation for ICSI, IVF, assisted reproductive technology
Oocyte preparation for ICSI, IVF, assisted reproductive technology

Recommended Products for Oocyte Preparation and Quality

ZEISS Stemi 508
Stemi 508 is your compact stereomicroscope, that fits in any standard Laminar Flow Box. The large working distance offers ample space: you can conveniently handle and manipulate oocytes.The operation of the microscope is easy and intuitive. Due to the optional Thermo plates you can keep your samples at 37 °C while they are outside the incubator.

ZEISS SteREO Discovery.V8
SteREO Discovery.V8 is equipped with open interfaces, that allows for integration inside your laminar flow box. Its modular design and extensive accessories offer you a variety of options to set up your workplace to your exact requirements. The impressive stereoscopic image helps you to better observe, and manipulate your oocytes. You get a high-resolution, high-contrast, and
apochromatically corrected microscopic image – that is your key for taking the right decisions. With its 8× zoom, you can quickly change from the overview down to the magnified detail, while staying perfectly in focus. SteREO Discovery.V8 combines robustness, ergonomy and brilliant optics, providing optimal reliability in your workflow.