Explore Life Science Imaging Solutions for Your Core Facility

Imaging solutions and techniques for life sciences

The breadth of equipment available in your imaging facility is key in defining the impact, success and growth of your core lab. Providing the right range of solutions ensures your userbase is large and varied and that instrument usage is high so that your return on investment is achieved quickly. Continued development and innovation at ZEISS ensures that you have a cutting-edge range of tools to set your core facility apart and provide the flexibility and reliability that you need to keep your users coming back.  

Live Imaging

Since the routine use of fluorescent proteins began in the 1990s, the drive to image live samples has revolutionized our understanding of modern biology. Live sample imaging has established itself as one of the cornerstones of the light microscopy core facility and this growing requirement is one of the reasons the number of publications invoking these approaches increases annually.

Phototoxicity is a major consideration for live imaging. Phototoxic damage caused by excessive light exposure can cause physiological changes that impact the integrity of the samples and the reliability of the experimental results. For this reason, the trade-offs between resolution, speed and gentleness need to be carefully considered for live experiments.

Different experiments with live specimens are served with different approaches and selecting the optimal combination of instrument and sample mounting is key to success

Whether you want to capture long-term volumetric data of coverslip mounted samples, acquire fast time-lapse movies with super resolution, follow developmental processes in embryos, or have the flexibility to address all of these requirements, the ZEISS range of solutions provides the flexibility you need. Read on to learn more about the top microscopes for live imaging for your core lab.

Minimizing light interaction with your live specimens is essential for minimizing phototoxic damage and allows longer imaging with more timepoints. Image generated using AICS-0013 (laminB1-mEGFP) from the Allen Institute, Seattle, USA using ZEISS Lattice Lightsheet 7

Live Imaging

Recommended Microscope Systems

Next Generation Confocal Microscopes with Fast, Gentle Multiplex Imaging

ZEISS LSM 9 Family with Airyscan 2

ZEISS LSM 9 Family with Airyscan 2

Next Generation Confocal Microscopes with Fast, Gentle Multiplex Imaging

At the center of the core imaging facility, the confocal microscope is the platform that provides users with the most flexibility to address their varied questions.

With a wide range of samples to image and diverse user skills, providing an accessible, flexible, and easy to use approach to live imaging in the facility is vital.

Provide the option of super resolution live imaging with gentleness unmatched by any confocal instrument without needing to train your users on any other imaging platform.

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Long-term Volumetric Imaging of Live Cells

ZEISS Lattice Lightsheet 7

ZEISS Lattice Lightsheet 7

Long-term Volumetric Imaging of Live Cells

For coverslip mounted specimens, imaging for hours or days at a time with minimal phototoxic damage provides the opportunity to discover new biology.

Lattice Lightsheet 7 provides the unique combination of fast, volumetric imaging with extremely gentle acquisition, an approach that promises to completely revolutionize the field of live cell imaging.

Offer this incredible live imaging capability and empower your users to immediately benefit without needing to reconfigure experiments or change any sample preparation.

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Automated Microscope for Live Cell Imaging

ZEISS Celldiscoverer 7

ZEISS Celldiscoverer 7

Automated Microscope for Live Cell Imaging

Getting quality data from live specimens without needing to be an expert in microscopy opens imaging to a much wider audience.

Celldiscoverer 7 combines the ease-of-use of an automated boxed microscope with the image quality of a classic inverted research microscope, a perfect match in the core imaging lab.

Allow the stability, reliability and reproducibility of Celldiscoverer 7 to empower your users to optimize and upscale their live imaging experiments and reduce your training load.

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Open and Flexible Platform for Live Imaging with AI-assisted Experiment Start-up

ZEISS AI Sample Finder automates the entire sequence from sample placement and focus adjustment to the identification of relevant sample areas.

ZEISS Axio Observer

Open and Flexible Platform for Live Imaging with AI-assisted Experiment Start-up

Providing an easy-to-use solution for live imaging with the flexibility you need for a wide range of experiments is essential in the core imaging facility.

Axio Observer provides powerful workflows driven by artificial intelligence from which each and every user can benefit.

As the live imaging needs of your users grow, so too does the Axio Observer since you can expand the platform to 3D sectioning and super resolution. Freely configure your experiments and enjoy a multitude of upgrade paths.

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Live Imaging System with Unprecedented Resolution

ZEISS Elyra 7 with Lattice SIM²

ZEISS Elyra 7 with Lattice SIM²

Live Imaging System with Unprecedented Resolution

The combination of super resolution and live imaging is increasingly used to explore biological processes, but the challenges associated with sample preparation and management have historically limited the possibilities.

Lattice SIM with SIM² processing enables fast live imaging of 3D volumes with up to 60 nm laterally and in multiple colors.

Bring the very latest in live super resolution imaging to your core facility and empower your users to capture new insights without any need to change their sample preparation.

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Multiview Imaging of Living and Cleared Specimens

ZEISS Lightsheet 7

ZEISS Lightsheet 7

Multiview Imaging of Living and Cleared Specimens - Flexible. Robust. Easy to Use.

To image whole living model organisms, tissues and cells as they develop. Light sheet fluorescence microscopy (LSFM) with its unique illumination principle is ideal for fast and gentle imaging of such specimens.

The exceptional stability of Lightsheet 7 lets you observe living samples over extended periods of time – even days – with less phototoxicity than ever before.
Lightsheet 7 includes the flexibility to observe your sample from the optimal angle or to combine image data from multiple angles.

What’s more, you can employ this technique to image very large optically cleared specimens in toto, and with subcellular resolution.
Complement your classical acquisition tools and address the needs of a very wide userbase in your core facility by providing optimal light sheet imaging of both live and cleared specimens.

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Super Resolution Light Microscopy

Super resolution light microscopy provides access to subcellular structural information by overcoming the diffraction limit. Observation of subcellular structures at these small scales has shown itself to be essential in biomedical research to better understand biological processes.

Rapid developments in technology have significantly expanded the range of applications and sample types that can benefit from super resolution light microscopy. This has led to instruments that are less niche and can more comprehensively address the demands of a wide userbase. These developments in technology have also reduced the requirement for specialised or complex sample preparation and this ensures that facility users can simply switch to super resolution without having to reconfigure experiments. This combination of factors has led to super resolution instruments now frequently complementing existing imaging approaches in the core facility to meet the demands of a varied userbase.

Whether you want to capture structural insights at <20nm resolution, perform live imaging at <120nm or meet multiple demands using a single instrument, the ZEISS range of solutions provides the flexibility you need. Read on to learn more about the top microscopes for super resolution imaging for your core lab.

Super resolution microscopy is now a mainstay in the imaging facility since it provides unique high-resolution insights in multiple colors

Super Resolution Light Microscopy

Recommended Microscope Systems

Fast and Gentle 3D Super Resolution Microscopy

ZEISS Elyra 7 with Lattice SIM²
ZEISS Elyra 7 with Lattice SIM²
Fusion proteins acquired with Elyra 7 using super resolution microscopy with fluorescence
Fusion proteins acquired with Elyra 7 using super resolution microscopy with fluorescence

ZEISS Elyra 7 with Lattice SIM²

Fast and Gentle 3D Super Resolution Microscopy

Super resolution requirements in the core facility continue to expand as experiments demand ever higher resolution at ever faster speeds.

ZEISS Elyra 7 provides a range of super resolution techniques on one platform to provide for applications as diverse as fast optical sectioning of 3D samples, single molecule localization microscopy (SMLM), and multicolor acquisitions in live specimens down to 60 nm resolution.

A truly multipurpose tool, Elyra 7 provides users with maximum flexibility for super resolution across length scales and sample types to ensure consistent and maximum usage in your facility.

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Next Generation Confocal Microscopes with Fast and Gentle Multiplex Imaging

ZEISS LSM 9 Family with Airyscan 2
ZEISS LSM 9 Family with Airyscan 2
Kidney transverse slice  Courtesy Pieter Vanden Berghe, LENS & CIC, University of Leeuwen, Belgium
Kidney transverse slice

ZEISS LSM 9 Family with Airyscan 2

Next Generation Confocal Microscopes with Fast, Gentle Multiplex Imaging

Expanding the capability of your confocal microscope to super resolution increases the range of applications that can be addressed without needing to move to a different imaging system.

The Airyscan 2 detector with jDCV not only extends the resolution performance of the LSM 9 family of confocal microscopes to 90 nm but also significantly increases the signal-to-noise ratio and speed, so this super resolution capability comes along with gentle, fast acquisition.

Extend the potential of your confocal platform to super resolution and allow your users to expand their experimental opportunities without needing to train them to use a different platform.

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Resources

White Papers

Technology Note

Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy

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ZEISS LSM 9 Family with Airyscan 2

Multiplex Mode for Fast and Gentle ConfocalSuperresolution in Large Volumes

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High Resolution Structural Imaging with Electron Microscopy

Exploring samples using electrons provides a wealth of structural information about your specimen. The power of the resulting information can be used alone, or in combination with microscopy data from other technologies to uncover details of how structure and function relate to each other.

Life science specimens pose unique challenges for electron microscopy since they are prone to charging and often have very low contrast prior to staining. They are also extremely delicate thus demanding low voltage acquisitions. Ensuring you can achieve high contrast even at low voltages is vital for good image quality, as is minimizing the artifacts generated by charging.

Stepping from 2D images to 3D structural volumes provides valuable insights into biological structure that are unavailable in single sections. While historically it has been a challenging and manual task to acquire 3D datasets using EM, advances in scanning electron microscopy and correlated technology such as X-ray microtomography and FIB-SEM are rapidly changing this.

A few of the approaches that are generating an exciting wave of structural explorations using bio-EM in 2D and 3D include:

  • SBFSEM (serial block face scanning electron microscopy) for physical sectioning of the sample in situ
  • Array tomography for sectioning and acquisition, followed by 3D reconstruction of many hundreds of single sections
  • Targeted sectioning of biological samples – either cryo preserved (vitrified) or conventional (embedded in resin) – using focused ion beam SEM
  • STEM (scanning transmission electron microscopy) to capture electrons through thin specimens

The ZEISS range of instruments provides the performance you demand with the ease of capture that means all facility users can benefit from the capability of high resolution 2D or 3D images.

Automated 3D acquisition of resin embedded samples provides powerful insights into 3D structure. Minimizing charging artifacts with approaches such as Focal Charge Compensation is vital to achieve the highest quality data from your life science specimens. 3D dataset of lung tissue acquired using Focal Charge Compensation courtesy of NCMIR.

High Resolution Structural Imaging

Recommended Microscope Systems

Modular SEM Platform for Intuitive Operation, Routine Investigations and Research Applications

ZEISS EVO Family
Cellular structure of a cross-section of an orange
Cellular structure of a cross-section of an orange

ZEISS EVO Family

Modular SEM Platform for Intuitive Operation, Routine Investigations and Research Applications

Support your users with an entry level, easy to use SEM with both environmental and extended pressure modes to broaden the sample range to bio-EM water-based samples such as insects and leaves.

Accommodate samples up to 30 cm in diameter and choose to optimize imaging conditions for each experiment by selecting the tungsten or lab6 emitter.

Rest assured that the capability of the EVO family can grow as the needs of your facility develop with multiple routes to upgrade.

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High Quality Imaging and Advanced Analytical Microscopy

ZEISS Sigma 300 Field Emission Scanning Electron Microscope with Gemini column and Oxford detectors
ZEISS Sigma 300 Field Emission Scanning Electron Microscope with Gemini column and Oxford detectors
Human retina snapshot. Astrocyte marked in turquoise in ultrastructure.
Human retina snapshot. Astrocyte marked in turquoise in ultrastructure.

ZEISS Sigma FE-SEM Family

For High Quality Imaging and Advanced Analytical Microscopy

Move into field emission (FE) technology with the proven Gemini column and use beam booster technology to achieve the most optimal image quality, even in low contrast samples.

You will benefit from multiple detector options including the on-axis Inlens detector that further drives the efficiency of low kV imaging. Include the option of the STEM detector to generate TEM-like images and extend the available imaging modes to include darkfield, brightfield, HAADF and more.

Enjoy flexible sample management with high vacuum and variable pressure options available and have full sample positioning flexibility using the Cartesian 5 axis stage.

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Highest Demands in Low Voltage Sub-nanometer Imaging, Analytics and Sample Flexibility

ZEISS Gemini FE-SEM
ZEISS Gemini FE-SEM
SARS-CoV-2 grown in a tissue culture and inactivated by chemical fixation.
SARS-CoV-2 grown in a tissue culture and inactivated by chemical fixation.

ZEISS Gemini FE-SEM Family

For Your Highest Demands in Low Voltage Sub-nanometer Imaging, Analytics and Sample Flexibility

Address your most demanding of specimens by providing the highest level of contrast for your users with proven Gemini column performance. The beam booster of the Gemini column works in combination with the on-axis InLens detector and Eucentric stage to deliver the highest possible quality imaging at low kV.

Choose from an array of detectors including secondary electron (SE) and InLens SE detectors for topography, back scattered detector (BSD) and InLens BSD for compositional studies, cathode luminescence (CT) detector for exploring autofluorescence, and benefit from additional compatible methods such as low kv EDX imaging for exploring particles like nano particles or titanium dioxide.

The scanning TEM (STEM) detector also provides TEM like imaging to further extend the functionality. Use as either a stand alone approach or as a means of screening sample quality prior to moving to the TEM.

Provide optimal image quality and maximum flexibility for capturing the finest details from the wide range of samples of your users.

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FIB-SEM for High Throughput 3D Analysis and Sample Preparation

ZEISS Crossbeam FIB-SEM
ZEISS Crossbeam FIB-SEM
3D reconstruction of algal Golgi body based on FIB-milling raw data.
3D reconstruction of algal Golgi body based on FIB-milling raw data.

ZEISS Crossbeam Family FIB-SEM

For High Throughput 3D Analysis and Sample Preparation

Focused Ion Beam SEM is increasingly the tool of choice for 3D visualization at the highest resolution and for preparing samples for subsequent exploration using techniques such as TEM. In the core imaging facility, providing a FIB-SEM tool with the flexibility to address a wide range of sample types is key for attracting a userbase that will guarantee maximum usage of the instrument.

The ZEISS Crossbeam family enables imaging of both cryo-preserved and ambient specimens. The proven Gemini column technology also provides the highest contrast and best possible image quality, even at the low voltages that life science samples demand.

During acquisition of 3D runs, ZEISS Crossbeam enables simultaneous milling and viewing so you can see the images as they are captured. This allows dynamic decisions during acquisition regarding the field of view or the ideal location for TEM lamella preparation.

Provide maximum flexibility for high resolution 3D EM acquisition in your core facility and allow your users to benefit from the best possible image quality in 3D.

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Centimeter Scale, Nanometer Resolution At Revolutionary Speed

ZEISS MultiSEM 506
ZEISS MultiSEM 506
Ultrathin mouse brain section
Ultrathin mouse brain section

ZEISS MultiSEM 505/506

Centimeter Scale, Nanometer Resolution At Revolutionary Speed

Empower your users to scale up their SEM imaging by using up to 91 parallel electron beams and capture cm size regions with nanometer resolution. This is your solution for acquisition of massive 3D volumes at unprecedented collection speeds.

ZEISS MultiSEM enables you to achieve imaging volume in 4 weeks that would take many years using a FE-SEM. Combine MultiSEM with array tomography for samples such as brain tissue for connectomics, where complex synaptic networks assembled by billions of neurons are studied.

Provide your users with a solution that enables a realistic timeline for scaling SEM acquisitions to address questions that would otherwise take many years to explore.

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Perform Block Face 3D Imaging of Your Biological Samples

Gatan 3View for ZEISS FE-SEMs
Gatan 3View for ZEISS FE-SEMs
Reconstructing the 3D volume from single x-y slices allows the visualization of planes which were not imaged directly (x-z, y-z). / Courtesy Naomi Kamasawa – Max Planck Florida Institute and Ryuichi Shigemoto – National Institute for Physiological Sciences Okazaki, Japan

3View® for Your ZEISS FE-SEMs

Perform Block Face 3D Imaging of Your Biological Samples

Exploring sample structure using 3D SEM offers a completely different range of biological insights. Serial block face imaging (SBFSEM) provides an automated means of capturing 3D volumes of resin embedded specimens

Incorporating the proven Gatan 3View system into your ZEISS FE-SEM for SBFSEM enables facility users' easy access to 3D data in a wide range of specimens.

Sample charging can cause major challenges in terms of data quality, particularly when imaging specimens with areas of bare resin. The Gatan 3View system can be combined with Focal Charge Compensation, which dramatically minimizes charging artifacts so that image quality is not compromised.

Support your users in expanding their SEM explorations to 3D and benefit from high quality 3D datasets in an easy-to-use solution.

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Resources

White Papers

White Paper: Correlative Protein Localization in Yeast

High-Resolution Localization of Fluorescent Proteins Using Shuttle & Find for Superresolution and Scanning Electron Microscopy.

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ZEISS Correlative Microscopy Solutions

A Publication Reference List

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Non-destructive Structural Imaging Using X-Rays

The non-destructive capture of 3D data using X-rays provides a valuable means of assessing the structure of biological specimens. For Life Science specimens, the key considerations for X-ray imaging are contrast and resolution. Biological samples usually have low density and the relative density of the structures of interest is similar. This drives the requirement for high-contrast capability to ensure precise differentiation of these structures. The resolution of the final image is also crucial for visualizing the structures of interest in enough detail and as such the ideal lab solution provides high-contrast and high-resolution data without the requirement to trim the sample to a smaller size.

This is where X-ray microscopy (XRM) plays a key role. Unlike traditional CT systems, X-ray microscopes use technology similar to that used at the synchrotron to generate magnification. This key difference results in a significant improvement in image quality and resolution when compared to classical CT approaches and brings great complementary value to the core imaging facility as it provides structural insight without physically sectioning the sample.

Whether the goal is to glean structural insight from a wide range of specimens, to check your sample preparation and integrity before further analysis, perhaps even at the synchrotron, or to identity regions of interest for subsequent exploration using electron microscopy, the ZEISS range of X-ray microscopes provides the quality and contrast you need for the most challenging specimens. Read on to learn more about the best microscopes for non-destructive structural imaging for your core lab.

X-ray microscopy enables non-destructive, high resolution, 3D acquisition of a wide range of life science specimens.
X-ray microscopy enables non-destructive, high resolution, 3D acquisition of a wide range of life science specimens.

Non-destructive Structural Imaging Using X-Rays

Recommended Microscope Systems

3D X-ray Microscopy for Faster Submicron Imaging of Intact Samples

ZEISS Xradia 620 Versa. Synchrotron-quality imaging in your lab.
ZEISS Xradia 620 Versa. Synchrotron-quality imaging in your lab.
Full field of view (FFOV) imaging of an unstained mouse embryo.
Full field of view (FFOV) imaging of an unstained mouse embryo.

ZEISS Xradia Versa family of X-ray microscopes

3D X-ray Microscopy for Faster Submicron Imaging of Intact Samples

Offering the state-of-the-art in 3D X-ray microscopy, the Xradia Versa family of X-ray microscopes (XRM) expands the boundaries of your non-destructive sub-micron scale imaging with an array of features and customization options, including advanced reconstruction techniques, autoloader for large sample runs, software for intelligent analysis, special sample protection, and more. Xradia solutions have been designed for ease of use for the busy lab.

Where traditional tomography relies on single-stage geometric magnification, Xradia Versa platforms feature a combination of unique two-stage magnification optics and a high flux X-ray source to produce submicron scale resolution images faster. Unique Resolution at a Distance (RaaD) architecture enables high resolution 3D imaging of larger, denser samples. Use the unique Scout-and-Zoom technique to rapidly produce a full field of view scan of your model to determine a region of interest to be scanned at higher resolution.

Ready for larger sample imaging now? Add the FPX flat panel display for large model imaging. Keep your users coming back by providing the highest resolution and contrast without the need to reduce the size of the sample for a wide range of specimens.

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Increase Your Productivity with Class-leading Performance

ZEISS Xradia Context  Class-leading and fully upgradeable microCT platform for rapid, large-sized imaging and return-on-investment
ZEISS Xradia Context - Class-leading and fully upgradeable microCT platform for rapid, large-sized imaging and return-on-investment
Intact jaw of a black bear, scanned with Xradia Context microCT.
Intact jaw of a black bear, scanned with Xradia Context microCT.

ZEISS Xradia Context microCT

Increase Your Productivity with Class-leading Performance

Using microCT, the internal structure or quality of staining of a wide range of samples can be assessed without destroying your sample.

ZEISS Xradia Context is an advanced large field-of-view, non-destructive 3D microCT system that provides high contrast imaging for samples ranging from millimeters to tens of centimeters in size, perfect for samples as diverse as organoids, bones, soft tissues and small animal models.

Provide powerful and easy to use microCT imaging in your facility. Fully-upgradeable to Xradia Versa platforms, Context enables you to rest easy that as the requirements of your users change, so the capability of the Xradia system can extend to a wide range of advanced capabilities so your investment is future proof.

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Extending the Reach of Non-destructive Nanoscale Imaging

ZEISS Xradia 810 Ultra Synchrotron-quality imaging at the highest resolution in your lab
ZEISS Xradia 810 Ultra – Synchrotron-quality imaging at the highest resolution in your lab
3D rendered view and zoom region cross section of a Cyclanthus bipartitus seed.
3D rendered view and zoom region cross section of a Cyclanthus bipartitus seed.

ZEISS Xradia Ultra X-ray microscope

Extending the Reach of Non-destructive Nanoscale Imaging

Extending the resolution of non-destructive X-Ray imaging to 50nm, the ZEISS Xradia Ultra X-ray microscope provides non-destructive imaging possibilities at resolutions unachievable with other lab-based technology.

The ZEISS Xradia Ultra provides both absorption and Zernike phase contrast acquisition modes to ensure a diverse range of specimens can be imaged using both stained and unstained sample preparations. To assist with imaging this diverse range of samples, the Scout-and-Scan Control System comes with an easy workflow-based user interface to ensure that this powerful level of capability is fully embraced by users of the imaging facility, even those with less of experience of microscopy.

Whether your goal is studying cellular and subcellular structure, looking at nanoparticle uptake or biomaterial nanostructure or non-destructively exploring your specimen with nano resolution, ZEISS Xradia Ultra provides unique possibilities. Reach the nanoscale without the need for complex sample preparation and provide exciting imaging capability to fulfil the needs of a huge and varied userbase.

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Resources

White Papers

Beyond Histology

with 3D X-ray Microscopy

pages: 5
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ZEISS X-ray Microscopes - Whitepaper

A Reference List

pages: 32
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ZEISS Xradia Versa X-ray microscopes

3D Quantitative Histology of Zebraish

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Multi-modal Imaging

Thorough investigation of biological samples often requires the use of more than one imaging approach. The imaging facility is ideally structured to offer the range of tools and workflows that users need for complex experiments. The goal may be exploration of structural interconnectivity or function of cellular components. There may be a desire to find rare events using one imaging approach before further interrogation by another or explore structure over multiple length scales. The process of multi-modal imaging has historically been both time consuming and labor intensive, and often requires the skills of highly trained technologists. In addition, once data are acquired from multiple instruments, visualization and alignment of those images with different pixel dimensions and file types can be challenging.

As the leading manufacturer of imaging solutions across the range of length scales, from light to X-ray to electron microscopy, ZEISS has created easy solutions for multi-modal imaging to guide you through all possible workflows.

Guided hardware solutions ensure you can easily support your users with multi-modal workflows between different instruments in your facility. In addition, ZEN Connect software provides a means of not only storing the data from each of the technologies in context, with a record of where each piece of valuable information resides relative to the others, but also offers a means of aligning these data in three dimensions so that valuable insights can be revealed. You can import data from any microscope system into ZEN and results from approaches other than imaging can even be added so your users keep a record of all their data about each sample in one place. Read on to learn more about the best solutions for multi-modal imaging for your core lab.

Using the power of multiple acquisition approaches enables different and new scientific questions to be addressed and storing that data in context is vital to keep track of how these data relate to each other.

Multi-modal Imaging

Recommended Microscope Systems

Connect Widefield or Confocal Fluorescence to Electron Microscopy under Cryo Conditions

TEM prep slusher of the Quorum Prepdek® workstation with ZEISS Perspex lid; components of the ZEISS Cryo Accessory Kit
TEM prep slusher of the Quorum Prepdek® workstation with ZEISS Perspex lid; components of the ZEISS Cryo Accessory Kit.
Plunge-frozen adenocarcinoma cells grown on sapphire discs.
Plunge-frozen adenocarcinoma cells grown on sapphire discs.

ZEISS Correlative Cryo Workflow

Connect Widefield or Confocal Fluorescence to Electron Microscopy under Cryo Conditions

Cryo microscopy allows the examination of cellular structures in their near-to-native state.

ZEISS Correlative Cryo Workflow connects widefield, laser scanning, and FIB-SEM in a seamless and easy-to-use process. The solution provides hardware and software optimized for the needs of correlative cryogenic workflows, from localization of fluorescent macromolecules to high-contrast volume imaging and on-grid lamella thinning for cryo electron tomography.

This workflow ensures that the valuable insights available from using cryo electron microscopy can be efficiently reached by all facility users.

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Connecting Non-destructive 3D X-ray Imaging with High Resolution Electron Microscopy (EM)

Perform large area imaging with ZEISS SEM, FE-SEM & FIB-SEM
Perform large area imaging with ZEISS SEM, FE-SEM & FIB-SEM.
Acquire EM Images Easier and Faster than Ever Before
Acquire EM images easier and faster than ever before.

ZEISS Atlas 5

Connecting Non-destructive 3D X-ray Imaging with High Resolution Electron Microscopy (EM)

Connecting imaging modalities such as X-ray imaging and high resolution EM enables the selection of target specific regions of the sample for EM acquisition and the generation of multiscale datasets with complementary information from each modality.

ZEISS Atlas 5 connects non-destructive 3D X-ray imaging to identify regions of interest for subsequent high resolution 2D or 3D EM imaging.

Maximize the efficiency of moving between instruments by employing the easy to use Atlas 5 software and allow your users to benefit from targeted EM acquisition.

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3D Correlated Light and Electron Microscopy for Serial Sections

ZEN Correlative Array Tomography
ZEN Correlative Array Tomography
Correlative 3D images show the distribution and orientation of plasmodesmata within the cell walls of root nodules influenced by symbiotic nitrogen. Courtesy D. Sherrier, J. Caplan, S. Modia, University of Delaware, USA

ZEN Correlative Array Tomography

3D Correlated Light and Electron Microscopy for Serial Sections

Array tomography is the physical sectioning of a sample onto tape for subsequent image capture and reconstruction. The approach provides the route for exploring large volumes of cell and tissue architectures with high levels of 3D structural detail.

Providing an automated means of capturing the serial sections significantly speeds up the image acquisition process. ZEN Correlative Array Tomography for serial sections connects overview images that are acquired using your light microscope with the high-resolution images that you capture with your electron microscope, and this enables automated capture and reconstruction of these datasets.

Allow your users to collect up to 1000 serial sections in a single day and automatically image and collate the datasets to gain unique 3D insights.

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Overlay and Organize Images from Any Source

ZEISS ZEN Connect Overlay and Organize Images from Any Source.
ZEISS ZEN Connect – overlay and organize images from any source.

ZEN Connect

Overlay and Organize Images from Any Source

When capturing data from multiple different imaging technologies, it can be challenging to align the various acquisitions and manage the resulting multi-modal datasets.

ZEN Connect not only enables aligned acquisition of datasets across different platforms but also provides a central location for keeping all of these datasets in context.

Provide facility users with a central platform for aligning and overlaying images from any source so you never need to worry about losing the context of their data.

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Drosophila slice stained for muscle (WeeP-MHC-GFP), nervous system & synapses (Anti-HRP-Cy3) and synaptotagmin (Anti-synaptotagmin-Cy5). Imaged using the Elyra 7 at different length scales and visualized using ZEN Connect. Courtesy of Sean Sweeney, Beatrice Rix, Peter O'Toole and Grant Calder, Department of Biology, University of York, UK

Resources

Webinars

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White Papers

Correlative Microscopy Protocols

A Reference Guide to Correlative Sample Preparation

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White Paper: Functional and Structural Investigation of Songbird Brain Projection Neurons with "Shuttle & Find"

Correlative Microscopy in Life Sciences

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ZEISS Correlative Microscopy Solutions

A Publication Reference List

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file size: 702 kB

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Automated, Efficient Imaging

The drive for reproducibility and statistical robustness has fueled an increase in automated, efficient microscopy to reduce user bias and quickly increase the number of sample points for statistical analysis. Providing imaging solutions with reduced complexity is also extremely advantageous for users who are not experts in microscopy and who greatly benefit from having access to instruments that offer optimized imaging without the need for hours of training.

Automated microscopes complement the range of equipment in the core imaging facility by providing a route for users to scale up their experiments without needing to know anything about microscopy at all. This goes alongside the capability to both operate and service these instruments remotely, essential requirements for instruments with such high levels of demand.

Historically, increasing throughput by using automation has meant a reduction in image quality, but advances in technology no longer engender this compromise. The most advanced automated instruments also provide optimal image quality, and since optimization is automatic, each and every user can benefit without needing technical support or training from the facility staff.

Whether your goal is high content screening, scanning many hundreds of slides, capturing multiplex data, acquiring time lapse movies, reducing the time you spend training users, or easy remote operation of your imaging, the ZEISS range of efficient solutions provides the flexibility you need.

Automated, efficient imaging provides an easy means of scaling experiments from a few sample points to many hundreds.
Automated, efficient imaging provides an easy means of scaling experiments from a few sample points to many hundreds. This supports the drive for statistical robustness and attracts users to the facility who do not have time to learn about microscopy.

Automated, Efficient Imaging

Recommended Microscope Systems

Automated Microscope to Add Efficiency to Your Fluorescence Imaging

Your Automated Microscope for Live Cell Imaging
Your Automated Microscope for Live Cell Imaging
Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo (Danio rerio).
Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo (Danio rerio). Sample Courtesy of J. Hartmann and D. Gilmour, EMBL, Heidelberg, Germany

ZEISS Celldiscoverer 7

Automated Microscope to Add Efficiency to Your Fluorescence Imaging

Training users to capture optimized, quality 3D data from each and every specimen doesn’t always make sense since many facility users do not have the time to become experts in microscopy.

Celldiscoverer 7 provides automated ease-of-use as well as optimal image quality, and this perfect match for the facility enables users to quickly configure and run experiments, even when remote from the system.

Select the stability, reliability and reproducibility of Celldiscoverer 7 to empower your users to optimize and upscale their imaging experiments without spending hours to train them.

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High-performance Slide Scanner for Fluorescence, Brightfield and Polarization

ZEISS Axioscan 7
ZEISS Axioscan 7 – High-performance Slide Scanner for Fluorescence, Brightfield and Polarization
The Bigger Picture: Slide Scanning within  the ZEN Environment
Automated slide scanning and detailed studies on other ZEISS microscope systems combined in a ZEN Connect project

ZEISS Axioscan 7

High-performance Slide Scanner for Fluorescence, Brightfield and Polarization

The routine scanning of slides complements other imaging approaches in the facility and provides a means of quick and easy scaling for experiments that generate large slide sets.

ZEISS Axioscan 7 combines qualities that you would not expect to see in a slide scanner: high speed digitization and outstanding image quality plus an unrivaled variety of imaging modes – brightfield, fluorescence, and polarization, full-spectrum, and more – all in a fully automated and easy to operate system.

The wide appeal of Axioscan 7 for managing these large slide sets ensures it is a valuable addition to the core facility and a sound revenue generator that will quickly cover the purchase outlay.

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ZEISS Celldiscoverer 7 - Application Note

Label-free Assays and Machine Learning Image Analysis

pages: 10
file size: 1641 kB

ZEISS Celldiscoverer 7 – Application Note

Analysis of three-dimensional cell culture using fast and sensitive widefield microscopy

pages: 6
file size: 1740 kB

ZEN Guided Acquisitionfor Life Sciences Applications

Guided Acquisition:Automate your microscopy - Detect rare events with ease

pages: 4
file size: 4886 kB

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Cleared Tissue Imaging

For large and dense specimens, tissue clearing is a powerful means of enabling imaging. By making the samples optically clear, it is possible to image much deeper without the need for physical sectioning.

There are many different protocols for clearing specimens, each with its own set of benefits and challenges, and selecting the best tissue clearing method is one of your first key decisions. Once your samples are transparent, the next important choice is which technology to use for imaging.

For some facilities, providing a dedicated solution for imaging cleared specimens may be an option, particularly if there is a key nucleus of users requiring this approach. But often this is not a practical choice and more versatile imaging approaches are a better solution to address the needs of a wider userbase.

Whether your cleared specimens are microns, millimeters, or centimeters in size, the ZEISS range of solutions provides optimal image quality so you can capture more data from a wide variety of specimens.

ZEISS Lightsheet 7
Cleared specimens provide unique insights into structure of a vast range of biological specimens. In this example, the mouse kidney cleared with iDISCO and imaged in Ethyl Cinnamate with Lightsheet 7. In red, DyLight 594 conjugated Tomatolection (vasculature and glomeruli), in green, auto-fluorescence (tissue anatomy). Sample courtesy of U. Roostalu, Gubra, Denmark

Cleared Tissue Imaging

Recommended Microscope Systems

Fluorescence Stereo Zoom Microscope for Large Fields

ZEISS Axio Zoom.V16 with ApoTome 2
ZEISS Axio Zoom.V16 with ApoTome 2

ZEISS Axio Zoom.V16

Fluorescence Stereo Zoom Microscope for Large Fields

The key for imaging large cleared specimens is having the flexibility to view specimens at different length scales while maintaining the brightness of the fluorescence.

Axio Zoom.V16 provides this flexibility and allows users to observe fluorescence images over the complete zoom range with the highest possible brightness.

This flexibility in field of view ensures users can quickly observe their large, cleared specimens and dynamically alter the resolution they need without requiring a laser-based instrument.

Its unique design makes Axio Zoom.V16 not only a perfect tool for the screening cleared samples, but also to image them in high detail, especially when combined with ApoTome 3.

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Next Generation Confocal Microscopes with Fast Multiplex Imaging

ZEISS Airyscan 2 for LSM 980
ZEISS Airyscan 2 for LSM 980

ZEISS LSM 9 Family with Airyscan 2

Next Generation Confocal Microscopes with Fast Multiplex Imaging

For mid-size cleared samples that require optical sectioning, classic confocal or multiphoton acquisition is very often the “go-to” technology of choice.

The ZEISS LSM 9 family provides high spectral flexibility and extremely high sensitivity. This combination is ideal for multicolor imaging of cleared specimens, while minimizing sample bleaching, vital across the large acquisition volumes.

Airyscan 2 will simultaneously provide you much higher resolution, improved signal-to-noise, and high speed imaging in multiple colours which is ideal for the large volumes you expect from cleared specimens.

The modular concept of the LSM 9 Family allows you to build flexibly so you always have the capability required to optimally support your users.

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Multiview Imaging of Living and Cleared Specimens

ZEISS Lightsheet 7
ZEISS Lightsheet 7

ZEISS Lightsheet 7

Multiview Imaging of Living and Cleared Specimens

The optical clearing method chosen for each sample depends on the type of tissue, your fluorescent labels and the size of the sample itself. Lightsheet 7 is designed to match all of these different conditions.

You can now image specimens at up to 2 cm in size at any refractive index between 1.33 and 1.58, and in almost all clearing solutions.

Lightsheet 7 lets you acquire overview images and data with subcellular resolution. Since the platform is designed for both cleared and living specimens, the range of users and applications that can be addressed ensures great demand in the facility for this versatile platform

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Automated Microscope to Add Efficiency to Your Fluorescence Imaging

ZEISS Celldiscoverer 7
ZEISS Celldiscoverer 7

ZEISS Celldiscoverer 7

Automated Microscope to Add Efficiency to Your Fluorescence Imaging

Training users to capture optimized, quality 3D data from each and every cleared specimen doesn’t always make sense since many facility users do not have the time to become experts in microscopy

Celldiscoverer 7 provides automated ease-of-use as well as optimal image quality. This perfect match for the facility enables users to quickly configure and run experiments on multiple cleared specimens, even in a remote format. Team Celldiscoverer 7 with Deconvolution or the LSM 900 with Airyscan 2 to provide an easy means of reaching optical sectioning in a wide range of cleared specimens

Allow the stability, reliability and reproducibility of Celldiscoverer 7 to empower your users to optimize and upscale their clearing experiments without spending hours on training

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Open and Flexible Platform for Cleared Sample Imaging with AI-assisted Experiment Start-up

ZEISS Axio Observer
ZEISS Axio Observer

ZEISS Axio Observer with AI-assisted Experiment Start-up

Open and Flexible Platform for Cleared Sample Imaging

Provide an easy to use solution for imaging cleared specimens with the flexibility you need for a wide range of sample types.

ZEISS Axio Observer provides powerful workflows driven by artificial intelligence from which each and every user can benefit.

As the needs of your user base grow, Axio Observer can grow with them since you can expand the platform to 3D sectioning and super resolution. Freely configure your experiments and enjoy a multitude of upgrade paths.

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Technology Note: ZEISS Lightsheet 7

How to Get Best Images with Various Types of Immersion Media and Clearing Agents

pages: 10
file size: 2117 kB

Application Note: Adjusting Refractive Index for Clearing Applications

Adjusting Refractive Index for Clearing Applications

pages: 5
file size: 1148 kB

Application Note: Improved Imaging of Cleared Samples with ZEISS Lightsheet Z.1

Refractive Index on Demand

pages: 7
file size: 664 kB

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