Deep Tissue Imaging of Biological Tissue Samples

Deep Tissue Imaging

Gaining Deeper Insight into Biological Tissue

Deep tissue imaging becomes necessary when you need to examine neuronal architecture and connections in thick brain slices or create 3D models of developing organisms and tissues. Your imaging depth is limited for conventional microscopes due to light being strongly scattered in biological tissue. You overcome this limitation using multiphoton and light sheet technology with dedicated imaging workstations and objectives from Carl Zeiss.

Multiphoton Microscopy

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Deep Tissue Imaging at High Resolutions With Multiphoton Microscopy

Multiphoton microscopy is your method of choice for deep tissue imaging at high resolution. A powerful pulsed laser with low-energy wavelengths is used for nonlinear excitation. Two or more photons must be absorbed simultaneously to excite one fluorescent molecule.

You benefit from multiphoton excitation in several ways. On one hand, fluorescence is virtually only generated in the focal plane of the biological tissue. This not only reduces phototoxicity and bleaching but also enables optical sectioning and 3D imaging. Furthermore, due to the use of long wavelengths you profit from reduced scattering and tissue absorption. Altogether, this technique allows you to image down to an outstanding depth of one millimeter.

With the NLO (Non-Linear Optics) add-on for the Carl Zeiss confocal laser scanning system LSM 980, you combine multiphoton capabilities with all the flexibility of confocal spectral imaging. By choosing an LSM MP system you obtain a dedicated multiphoton specialist for deep tissue imaging of your most demanding biological tissue samples and living organisms. Gain additional information on tissue structure without specific staining using supplementary methods. For example, profit from second harmonic generation to examine periodic structures like collagen and striated muscle or from third harmonic generation for lipid-water boundaries.

Scaling Done Right

The 'Scale' reagent renders brain tissue virtually transparent while preserving fluorescent proteins. Use your ZEISS multiphoton or laser scanning microscope together with our special 'Scale' objective LD Plan-APOCHROMAT 20x/1.0 to track neurons up to 6 millimeters deep into tissues. Read more

Live Imaging of Large Volumes With Light Sheet Fluorescence Microscopy

Lightsheet Z.1 is specially designed for large volume imaging. A unique sample holder allows you to rotate your specimen within the illuminating light sheet. Thus, you compensate for the physically limited light penetration depth by imaging from multiple views. Reconstruct a 3D model of your organism out of several images acquired from different angles with ZEN digital imaging software suite. Providing gentle illumination and incubation options, Lightsheet Z.1 is perfectly suited for the observation of living organisms and biological tissues over extended periods of time.

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