Image each Model Organism in Developmental Biology

Developmental Biology

Know Your Model Organism

Developmental biology is the study of how organisms grow and develop. This includes the genetic control of cell growth and differentiation to form tissues, organs and whole organisms. Developmental biologists study a host of model organisms including C. elegans, Drosophila, Arabidopsis, Zebrafish and mice. Due to the differences in object size and tissue composition, you face many challenges in your microscopy research when working with a model organism. 

Stereo and Zoom Microscopes - From Large Overview to Small Details

Your research questions in developmental biology are often in the context of the whole organism. The challenge is how to image fine details, while also capturing a larger overview. Zoom microscopes like Axio Zoom.V16 combine the large field of view of a stereomicroscope with the high resolving powers and bright fluorescence of a compound microscope. This enables brilliant visualization of fine structures within your entire organism of interest. For routine tasks, you profit from an ergonomic and easy-to-use stereomicroscope. Featuring a 8:1 zoom range the Greenough microscope Stemi 508 is best in its class. With sharp, three-dimensional images you easily inspect your model organism, sort for breeding, select individuals for your experiments and accomplish micro-dissection. For your demanding imaging tasks the SteREO Discovery family comprises stereomicroscopes with zoom ranges up to 20:1. With the touch panel SYCOP you operate your microscope easy and without moving your eyes from the eyepieces. To view complete model organisms with high fluorescence intensity choose the motorized zoom microscope Axio Zoom V.16. Thanks to a very high numerical aperture you achieve outstanding fluorescence brightness even in large object fields. Acquire multidimensional images and complement with ApoTome.2 for optical sectioning.

Confocals - Imaging in 3 Dimensions, Multiple Colors and Over Time

Confocal laser scanning microscopes (LSM) and spinning disk microscopes are powerful tools for three-dimensional and multicolor fluorescence imaging of your model organism. Use Cell Observer SD with spinning disk technology for gentle 3D imaging of your living organisms with unprecedented speed. The LSM 8 family of fast linear scanning confocals with GaAsP detectors now offers Airyscanning, a novel detection technology. You gain 1.7× higher resolution in all three dimensions – resulting in a 5× smaller confocal volume. And you push sensitivity beyond the limits of all conventional confocals. The compact and easy-to-use laser scanning microscope LSM 800 rivals high-end confocals, offering excellent image quality and demanding techniques such as spectral imaging and linear unmixing. For utmost flexibility you choose LSM 880. You save time on investigations into localization and interaction of proteins that require multiple fluorescent labels: You collect all these signals in one go. You perform simultaneous spectral detection in a single scan with the highest number of descanned or non descanned channels – including GaAsP technology. And you benefit from large fields of view and the highest speed of any linear scanning confocal – up to 13 fps.

With ZEN imaging software you configure most of your experiments automatically with Smart Setup. For image processing and evaluation you profit from advanced tools like 3D sectional view, orthogonal sectional view or stitching. 

Light Sheet Fluorescence Microscopy - Watch Life in 3D

As a developmental biologist you strive to watch living organisms develop over time. Phototoxic effects from traditional imaging methods often challenge your attempts. Light sheet fluorescence microscopy (LSFM, sometimes termed as selective plane illumination microscopy, SPIM) is a new method for gentle, deep and fast 3D imaging. Lightsheet Z.1 is tailored for long term live imaging. The light sheet illuminates just a thin volume of your specimen. This reduces phototoxicity to a minimum and enables optical sectioning by avoiding the excitation of out-of-focus light. In addition, parallel imaging of the focal plane with a camera allows extremely fast image acquisition compared to point-scanning microscopes. With Lightsheet Z.1 you monitor delicate organisms over several days and visualize populations of cells grow into tissues and early embryos.
Lightsheet Z.1 allows Multiview imaging of your sample: you view and image from exactly the right angle and later fuse different views in one 3D data set. With the patented pivot scanning you always get homogeneous illumination. 

3D Imaging of Microstructures

With electron microscopy you examine microstructures within organisms at the nanometer scale. Combine your scanning electron microscope from the Sigma or GeminiSEM family with a Gatan’s 3View ultramicrotome inside the chamber. The 3View system continuously cuts and images your whole embedded organism to create serial block face images in a process called SBF-SEM. Reassemble these images into a three-dimensional model for close inspection of cell and tissue organization, rivaling the resolving power of a transmission electron microscope thanks to unique GEMINI II electron optics.

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