Light Sheet Fluorescence Microscopy

Lightsheet Z.1

Fast, Gentle Imaging for Living Samples

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  • Introduction

    Long Term Multiview Imaging for Large, Living Samples with Light Sheet Microscopy

    Imagine you had access to an imaging system that could perform fluorescence imaging on large, living samples, with virtually no phototoxicity or bleaching and with high temporal resolution.

    That is exactly what Lightsheet Z.1 from Carl Zeiss does. The unique Multiview Light Sheet Fluorescence Microscope allows you to record the development of large, living samples and gently image them to deliver exceptionally high information content.

    It is also fast. Lightsheet Z.1 gives you the tools you need to work at unprecedented speed and acquire images of your whole sample volume at sub-cellular resolution – in a fraction of the time it takes using other techniques.

    Ask your contact at Carl Zeiss today about Lightsheet Z.1

  • Highlights

    Light Sheet Fluorescence Microscopy (LSFM) with Lightsheet Z.1

    Multiview Imaging. Delivering true flexibility

    • Use Multiview to find the ideal position of your specimen in relation to the detection objective lens
    • Image complete specimens by acquiring complementary information from different views, before recombining them in a simple post-processing step
    • Improve resolution of your processed dataset by recombining information from different views and use deconvolution to further improve the image quality.

     

    The best of all worlds. Optimized light sheet microscopy optics

    • Shape the illumination light sheet with combined cylindrical optics and a beam scanning mechanism
    • Rely on specially designed optics that give you the most homogenously illuminated optical section of any LSFM microscope
    • Protect your sample by using extremely low laser intensities to minimize bleaching

     

    Fluorescent imaging for large living samples

    • Create Multiview data sets using a new sample positioning approach.
    • Easily maintain physiologically relevant conditions and benefit from the improved combination of image information and control of environmental conditions.


    Ask your contact at Carl Zeiss today about Lightsheet Z.1

  • Applications

    A New World of Fluorescence Microscopy

    Perform previously impossible experiments
    Because Lightsheet Z.1 is the gentlest way to observe the development of living specimens, it opens up a new world of experiments for your research.

    These are just some of the light sheet microscopy applications that are now available to you.

    Morphogenesis and embryogenesis
    Perform fluorescence imaging of spatio-temporal patterns within cells during embryogenesis of model organisms such as zebrafish and Drosophila melanogaster.

    Organogenesis and cell dynamics
    Fast imaging of cellular dynamics in embryos and small organisms like cell migration, cardiac development, blood flow, vascular development, neuro-development or calcium imaging.

    3D cell cultures
    Benefit from live imaging of 3D cell cultures, spheroids and cysts, tissue and organotypic cultures. Use your images to analyse cell migration, expression patterns and cell proliferation.

    Imaging of marine organisms
    Use Lightsheet Z.1 for fluorescence imaging of marine organisms such as ciona, squid, plankton and flatworms.

    Structural features of fluorescently labeled living specimens
    Acquire detailed volume imaging of fixed and living specimens, for example zebrafish and Medaka fish.

    Ask your contact at Carl Zeiss today about Lightsheet Z.1

    Zebrafish Heart Development

    Zebrafish Heart Development: LSFM images from the beating heart of a 2-day old zebrafish over extended periods of time, delivering maximal frame rates (80 to 100 fps) with only minimal light exposure. The 2-channel fluorescence image dataset shows blood vessels and the endocardium labeled in red, the myocardium in green.

    Specimen provided by M. Weber and J. Huisken, MPI-CBG Dresden, Germany

    Complete LSFM Imaging of Zebrafish Embryo Development

    This entire 2-day old zebrafish was imaged from four angles and reconstructed using Multiview registration and fusion software. The fish expresses Tg (Bactin:H2A-mCherry) in the nuclei. The green channel shows autofluorescence.

    Specimen provided by M. Weber and J. Huisken, MPI-CBG Dresden, Germany

  • Concept

    Fast, Light-Efficient Data Acquisition

    Light sheet fluorescence microscopy or LSFM splits fluorescence excitation and detection into two separate light paths, with the axis of illumination perpendicular to the detection axis. That means you illuminate a single thin section of your sample at one time, generating an inherent optical section by exciting only fluorescence from the in-focus plane. No pinhole or image processing is required for light sheet microscopy.

    Light from the in-focus plane is collected on the pixels of a camera, rather than pixel by pixel as, for example, in confocal or other laser scanning microscopy approaches. Parallelization of the image collection on a camera-based detector lets you collect images faster and with less excitation light than you would with many other microscope techniques.

    In summary, LSFM combines the optical sectioning effect with parallel image acquisition from the complete focal plane. This makes 3D imaging extremely fast and very light efficient.

    Ask your contact at Carl Zeiss today about Lightsheet Z.1

  • Downloads

    Product Information (3.6 MB)

    Customer Report: Light Sheet Microscopy at the Harvard Center for Biological Imaging (0.5 MB)

    White Paper: Sample Preparation (7 MB)

    Product Information (interactive iBook; 76 MB)

    For the iBooks version: Either download the file to your computer and perform a manual sync with iTunes, or download the file directly onto your iOS device via WiFi and the Safari Browser.

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