ZEISS Microscopy

Revolutionize Your Confocal Imaging

Discover LSM 8 microscopes for fast and gentle live cell imaging

Image the smallest structures, catch the faintest signal, track the fastest processes – or do all of that at once. Get the unique combination of gentle superresolution imaging and high speed with ZEISS LSM 8 confocal microscopes:

  • Profit from our novel detector design: Airyscan gives you an instant 4 – 8× improvement of signal-to-noise
  • See the smallest details: Resolve structures of 120 nm (in x,y) and 350 nm (in z)
  • Track the fastest processes: Acquisition speeds of 27 fps (at 480x480, LSM 880 in Fast mode)
  • Protect your sample and save time: Acquire the entire fluorescent spectra of all your labels at once

Download the application note "ZEISS LSM 800 and LSM 880 with Airyscan, Imaging Biological Samples – a Reference List" to learn more about how you can profit from Airyscan. See how other researchers have used the unique features of ZEISS confocal microscopes and get inspired!

Want to get guides, technology notes and whitepapers about improving your confocal imaging? Check the box below the form to allow us to send you these and other microscopy relevant topics via email. You can unsubsribe anytime in the bottom of each email, in case you are not interested anymore.

Discover how you can profit in your research field

Cell Biology

Confocals, your workhorses for 3D imaging

  • Photo-activatable dyes and fluorescence proteins allow you to measure the dynamics and localization of protein populations over time
  • Fluorescence correlation studies within cells provide you with valuable information on protein clustering and dynamics
  • Benefit from faster scan rates and consistent image conditions
  • Your FRAP and photoactivation experiments profit from tools for manipulating freely definable ROIs with individual settings 

Neuroscience Research

Scaling deep into tissues

  • Profit from 34-channel parallel imaging across the complete wavelength to monitor up to 10 dyes simultaneously
  • Combine laser scanning functionality with an outstanding imaging depth thanks to nonlinear optics
  • Record intact neuronal networks in living animals or thick tissue specimens
  • To render brain tissue virtually transparent while preserving fluorescent proteins you apply clearing methods such as 'Scale S' to your sample
  • In combination with a range of special clearing objectives, you image to a depth of almost six millimeters within your tissue 

Developmental Biology

Imaging in 3D, multiple colors and over time

  • Gain 1.7× higher resolution in all three dimensions – resulting in a 5× smaller confocal volume
  • Save time on investigations into localization and interaction of proteins that require multiple fluorescent labels: collect all these signals in one go
  • Perform simultaneous spectral detection in a single scan with the highest number of descanned or non descanned channels – including GaAsP technology
  • Benefit from large fields of view and the highest speed of any linear scanning confocal – up to 27 fps (480x480 pixels, LSM 880 in Fast mode) 

See the difference between conventional confocal and ZEISS Airyscan

HeLa cells, Actin stained with Phalloidin-Alexa 546, AP3 with Alexa 488 - Courtesy of S. Traikov, BIOTEC, TU Dresden, Germany

 

Confocal

HeLa cells, Actin stained with Phalloidin-Alexa 546, AP3 with Alexa 488

Courtesy of S. Traikov, BIOTEC, TU Dresden, Dresden, Germany

HeLa cells, Actin stained with Phalloidin-Alexa 546, AP3 with Alexa 488 - Courtesy of S. Traikov, BIOTEC, TU Dresden, Germany

 

ZEISS Airyscan

HeLa cells, Actin stained with Phalloidin-Alexa 546, AP3 with Alexa 488

Courtesy of S. Traikov, BIOTEC, TU Dresden, Dresden, Germany

mouse brain sections of thy1-GFP line imaged using LSM 880, color coded projection - COPYRIGHT: Yi Zuo, UCSC

 

Confocal

Mouse brain sections of thy1-GFP line imaged using LSM 880, color coded projection

Courtesy of Yi Zuo, University of California, Santa Cruz, USA

Mouse brain sections of thy1-GFP line imaged using LSM 880 with Airyscan, color coded projection - COPYRIGHT: Yi Zuo, UCSC

 

ZEISS Airyscan

Mouse brain sections of thy1-GFP line imaged using LSM 880 with Airyscan, color coded projection

Courtesy of Yi Zuo, University of California, Santa Cruz, USA

B cell Fubulin<488 - Joseph Brzostowski, NIH/NIAID, LIG Imaging Facility, Bethesda, MD, USA

 

Confocal

B cell Fubulin<488

Courtesy of J. Brzostowski, NIH/NIAID, LIG Imaging Facility, Bethesda, MD, USA

B cell Fubulin<488 - Joseph Brzostowski, NIH/NIAID, LIG Imaging Facility, Bethesda, MD, USA

 

ZEISS Airyscan

B cell Fubulin<488

Courtesy of J. Brzostowski, NIH/NIAID, LIG Imaging Facility, Bethesda, MD, USA

A cricket embryo - Sample courtesy of: Cassandra Extavour Harvard University UK

 

Confocal vs. Airyscan

A cricket embryo. This is an egg-stage 22 Gryllus bimaculatus embryo, stained with phalloidin coupled to Alexa488.

Example Airyscan with NLO excitationThis phalloidin stained muscle in the cricket embryo is imaged with NLO laser excitation (900nm). When imaged with a GaAsP NDD the image shows only little detail, while with Airyscan, multiple structures are visible. Even DCV cannot enhance the quality accordingly.

Sample: courtesy of C. Extavour, Harvard University, UK

Tissue cross section stained with Eosin & Hematoxyllin - Olga Chumakova, UTHSCH, Medical School, Houston, TX

 

Confocal vs. Airyscan

Tissue cross section stained with Eosin & Hematoxyllin. Testis sections from male mice. Imaged with LSM 880 and Airyscan

Courtesy of O. Chumakova, UTHSCH, Medical School, Houston, TX, USA

Mammary gland transplanted outgrowth - Yang Lu (Daisy), Baylor College of Medicine, Houston, TX

 

Confocal vs. Airyscan

Mammary gland transplanted outgrowthGFP / RFP / BRDu Stain mammary ductal structures. PFA fixed and paraffin embedded

Courtesy of Yang Lu (Daisy), Baylor College of Medicine, Houston, TX, USA

Mouse brain sections of thy1-GFP line imaged using LSM 880, color coded projection - COPYRIGHT: Yi Zuo, UCSC

 

Confocal

Mouse brain sections of thy1-GFP line imaged using LSM 880, color coded projection

Courtesy of Yi Zuo, University of California, Santa Cruz, USA

Mouse brain sections of thy1-GFP line imaged using LSM 880 with Airyscan, color coded projection - COPYRIGHT: Yi Zuo, UCSC

 

Airyscan

Mouse brain sections of thy1-GFP line imaged using LSM 880, color coded projection

Courtesy of Yi Zuo, University of California, Santa Cruz, USA

Do you want to speak to our confocal imaging experts directly to get answers of how ZEISS confocal microscopes can benefit your research? Contact us!

ZEISS is offering attractive new packages of ZEISS LSM 8 confocal microscopes. Find out more and get in touch with us.