Press Release

New workflow solution for tem lamella preparation and volume imaging under cryogenic conditions

ZEISS correlative cryo workflow connects light and FIB-SEM microscopy to analyze cellular ultrastructure

3 February 2021 · 2 min read
  • Core Imaging Systems - Cryo Microscopy
  • Correlative Microscopy
Press Contact for ZEISS Microscopy Vybhav Sinha

Jena, Germany | 3 February 2021 | ZEISS Research Microscopy Solutions

With the release of ZEISS Correlative Cryo Workflow, ZEISS provides the life science research community with a new combined hardware and software solution for cryogenic microscopy. The workflow connects widefield, laser scanning, and FIB-SEM microscopes in a seamless and easy-to-use procedure. The solution provides hardware and software optimized for the needs of correlative cryogenic workflows, from localization of fluorescent macromolecules to high-contrast volume imaging and on-grid lamella thinning for cryo electron tomography.

Correlative cryogenic microscopy is an emerging technique for structural analysis of macromolecules in their cellular context. Because the ultrastructure of cells and tissues can be preserved free of artifacts, cryogenic microscopy allows the examination of cellular structures in their near-to-native state. However, this technique presents users with complex challenges, such as time-consuming preparation and imaging procedures, devitrification, ice contamination, or loss of samples.

“At ZEISS, we are generally working to ensure that researchers can collect their data faster and analyze them better. With the ZEISS Correlative Cryo Workflow, we are taking the next step towards simplifying and optimizing work processes for scientists so that they can fully concentrate on their research,” emphasizes Dr. Michael Albiez, Head of ZEISS Research Microscopy Solutions.
Various research fields, such as cell biology, cancer research, plant science, and developmental biology, benefit from the ultrastructural information acquired with cryogenic microscopy. ZEISS Correlative Cryo Workflow gives researchers easier access to this advanced technique, letting them faster evaluate the quality of their samples, acquire high-resolution, high-contrast 3D datasets, and streamline their TEM lamella preparation.

  • ZEISS LSM 900 and ZEISS Crossbeam combined in a correlative cryogenic workflow

    ZEISS LSM 900 and ZEISS Crossbeam combined in a correlative cryogenic workflow

  • Components of the ZEISS Cryo Workflow Accessory Kit

    Components of the ZEISS Cryo Workflow Accessory Kit

  • Correlative cryo dataset in ZEISS ZEN Connect

    Correlative cryo dataset in ZEISS ZEN Connect

Simplified workflow and safe transfer of samples

ZEISS Correlative Cryo Workflow connects widefield or confocal microscopes (ZEISS Axio Imager, ZEISS LSM 900/980 with Airyscan) and focused ion beam scanning electron microscopes (ZEISS Crossbeam) to enable volume imaging and efficient production of TEM lamellae. Dedicated accessories simplify the workflow and facilitate a safe transfer of vitrified samples between the microscopes. The components are compatible with the cryo-correlative microscopy stage Linkam CMS196V³ and the cryo system Quorum PP3010Z. Data management is assured by ZEISS ZEN Connect, the software for correlative microscopy. A series of processing tools help to enhance the imaging results.

Highest imaging performance across the entire workflow

Thanks to cryo-compatible objectives and the high sensitivity of the ZEISS Airyscan detector, ZEISS LSM systems enable the detection and localization of proteins and cellular structures at high resolution while gentle illumination prevents samples from devitrification. The FIB-SEM ZEISS Crossbeam microscopes provide high-contrast volumetric imaging – even without heavy metal staining applied to the samples. Both modalities provide valuable functional and structural information for a thorough understanding of ultrastructure.

Possible use at room temperature increases productivity

Unlike other solutions, the ZEISS microscopes involved in the workflow can be used not only for cryogenic microscopy, but also for room temperature applications. Converting the instruments from cryogenic to room temperature usage is done quickly and doesn’t require technical expertise. This flexibility gives users more time for their experiments. Imaging facilities benefit from better utilization and a faster return on investment.


ZEISS is an internationally leading technology enterprise operating in the fields of optics and optoelectronics. In the previous fiscal year, the ZEISS Group generated annual revenue totaling 6.3 billion euros in its four segments Semiconductor Manufacturing Technology, Industrial Quality & Research, Medical Technology and Consumer Markets (status: 30 September 2020).

For its customers, ZEISS develops, produces and distributes highly innovative solutions for industrial metrology and quality assurance, microscopy solutions for the life sciences and materials research, and medical technology solutions for diagnostics and treatment in ophthalmology and microsurgery. The name ZEISS is also synonymous with the world's leading lithography optics, which are used by the chip industry to manufacture semiconductor components. There is global demand for trendsetting ZEISS brand products such as eyeglass lenses, camera lenses and binoculars.

With a portfolio aligned with future growth areas like digitalization, healthcare and Smart Production and a strong brand, ZEISS is shaping the future of technology and constantly advancing the world of optics and related fields with its solutions. The company's significant, sustainable investments in research and development lay the foundation for the success and continued expansion of ZEISS' technology and market leadership. ZEISS invests 13 percent of its revenue in research and development – this high level of expenditure has a long tradition at ZEISS and is also an investment in the future.

With over 32,000 employees, ZEISS is active globally in almost 50 countries with around 30 production sites, 60 sales and service companies and 27 research and development facilities. Founded in 1846 in Jena, the company is headquartered in Oberkochen, Germany. The Carl Zeiss Foundation, one of the largest foundations in Germany committed to the promotion of science, is the sole owner of the holding company, Carl Zeiss AG.

Further information at


ZEISS Research Microscopy Solutions

ZEISS Research Microscopy Solutions is the world's only one-stop manufacturer of light, electron, X-ray and ion microscope systems and offers solutions for correlative microscopy. The portfolio comprises of products and services for life sciences, materials and industrial research, as well as education and clinical practice. The unit is headquartered in Jena. Additional production and development sites are located in Oberkochen and Munich, as well as in Cambourne (UK) and Dublin (USA). ZEISS Research Microscopy Solutions is part of the Industrial Quality & Research segment.

Press photo download

  • ZEISS Correlative Cryo Workflow

    ZEISS LSM 900 and ZEISS Crossbeam combined in a correlative cryogenic workflow

    File size: 178 KB
  • ZEISS Cryo Workflow Accessory Kit

    File size: 208 KB
  • Correlative cryo dataset in ZEISS ZEN Connect

    File size: 139 KB

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