ZEISS at Microscopy Conference 2013

From 3D Light to 3D Electron Microscopy

Joint Workshop on Correlative Microscopy for the Life Sciences

ZEISS in Collaboration with the Flanders Institute for Biotechnology (VIB) 

Ghent, Belgium - March 9 - 11

Life happens in 3D – and understanding biological context in the three dimensions has been a major driver in light microscopy in the past decades. In the process 3D imaging technologies such as laser scanning microscopy have become standard in every modern biosciences lab.

Today the trend to 3D continues towards highest resolution with modern field emission scanning electron microscopy (FE-SEM) based techniques enabling previously thought impossible insights to structural information. Automated FE-SEM technology has made a change to the way scientists approach 3D EM volumetric imaging by allowing for larger and thus scientifically relevant data sets. In the race to 3D understanding Correlative Microscopy is a key enabler activating the link between function and structure.

ZEISS in collaboration with the BioImaging Core Facility at the VIB Ghent are proud to host this joint workshop and scientific meeting on Correlative Microscopy and 3D SEM. The meeting is centered around scientific sessions covering a broad range of correlative microscopy and SEM methods and extending to data management approaches. In addition participants will have the opportunity to join instrument workshops and round table discussions.
Participants are encouraged to present their own scientific work in poster or oral presentations. A scientific panel will review abstracts submitted for oral presentation.
Don’t miss this exciting platform for exchange with researchers and industry to explore the new and emerging technologies and methods for correlative microscopy and 3D SEM in the life sciences.

Conference Center Het Pand
Onderbergen 1 (next to Sint-Michiels church), 9000 Ghent

Flanders Institute for Biotechnology (VIB)
Technologiepark 927, 9000 Ghent

  • Scientific Meeting

    Scientific Program

    Hear about the latest advances in correlative microscopy and SEM in life science.

    • Oral Presentations
    • Poster Session


    Live Workshops

    See for yourself, sign-ups for Live Demo Workshops are available for:

    • Correlative Array Tomography
    • 3View (SBEM)
    • FIB-SEM
    • Sample Preparation



    Meet us to discuss the challenges for correlative microscopy and SEM:

    • 3D microscopy, taming the data
    • 3D SEM - can we replace TEM?
    • The best way to CLEM, is there
      (Cryo CLEM, FIB-SEM, 3D-SEM,


  • Program


    Day 1 | Sunday, March 09th 2014
    Conference Center Het Pand, Onderbergen 1
    14:00 – 15:30 Arrival and Registration
    15:30 – 16:00 Welcome Speech from VIB & ZEISS
    16:00 – 17:00 Correlative PALM and EM: Options and Tradeoffs (Harald Hess / Ashburn )
    17:00 – 18:00 Toward Making the Invisible and Complicated Understandable: Microscopy Across Scales and Modalities (Mark H. Ellisman / San Diego)
    18:00 - 19:30 Reception
    Day 2 | Monday, March 10th 2014
    VIB, Technologiepark 927
    08:30 – 09:00 Transfer from PickUp Point in the City to the VIB
    09:00 – 09:30 Registration FSVM Building Zwijnaarde
    09:30 – 09:45 Welcome to VIB (Chris Guérin)
    Session I: FIB-SEM I
    09:45 – 10:20 FIB-Nanotomography for bio-medical applications: high-resolutionanalysis of cells and tissues (Marco Cantoni / Lausanne)
    10:20 – 10:40 Which Direction to Take for 3D CLEM: CLSM Combined with FIB-SEM, or Array Tomography? (Miriam Lucas / Zürich)
    10:40 – 10:55 Use of different methods of correlative light-electron microscopy and three-dimensional reconstruction of ultra-structures for the study of intracellular transport (Galina V. Beznoussenko / Milan)
    10:55 – 11:20 Coffee Break
    11:20 – 11:35 FLIPPER, A combinatorial probe for 3D-LM/(ST)EM analysis of large areas (Ben Giepmans / Groningen)
    11:35 – 11:55 Genetically encoded probes for light and EM imaging in brain tissue (Julian Ng / Cambridge)
    11:55 – 12:10 Achievements of the correlative light-electron microscopy in combination with the three- dimensional electron microscopy (A.A. Mironov / Milan)
    12:10 – 13:10 Lunch & Poster
    Session II: FIB-SEM II
    13:10 – 13:30 Correlative Light and Electron Microscopy: taking snapshots of the living at the ultrastructural level (Yannick Schwab / Heidelberg)
    13:30 – 14:05 Three-dimensional Imaging of Adherent Cells. a comparison of FIB-SEM, STEM and serial sectioning (Paul Walther / Ulm)
    14:05 – 14:30 Cryo-FIB-SEM: 3D imaging of cellular ultrastructure in native frozen samples (Wiebke Möbius / Göttingen)
    Workshops & Round Table Discussion
    14:45 – 17:15 Workshops (CAT, 3View, FIB-SEM, WetLab, Computing)
    Round Tables
    - 3D microscopy - taming the data tsunami
    - 3D SEM - can we replace TEM?
    - The best way to CLEM, is there one?
    Session III: Data Handling & Segmentation
    17:30 – 18:05 Modeling Brain Circuitry over a Wide Range of Scales (Pascal Fua / Lausanne)
    18:05 – 18:25 Interactive analysis of 3D EM data with ilastik (Anna Kreshuk / Heidelberg)
    18:25 – 18:40 Cell-Specific Visualization and Manipulation of Synapses In Vivo through Correlated Light and Electron Microscopy Combined with Reverse Genetics (Olivier Urwyler / Leuven)
    18:40 – 18:55 Comparative Connectomics: Analysis of anatomically derived connectivity data based on 3D electron microscopy (Dan Bumbarger / Tübingen)
    19:00 Evening Dinner Event – F.S.V.M Atrium
    21:30 Bus transfer to City
    Day 3 | Tuesday, March 11th 2014
    VIB, Technologiepark 927
    08:30 – 09:00 Transfer from PickUp Point in the City to the VIB
    Session IV: Other Roads to CLEM I
    09:30 – 10:00 Preservation of Fluorescence During Quick Freeze Substitution (Richard Webb / Queensland)
    10:00 – 10:30 Linking structure to function in cells: Correlation to integration, electrons to X-rays (Lucy Collinson / London)
    10:30 – 10:45 Correlative microscopy using SIMS: High-sensitivity high-resolution elemental mapping for biological and materials science applications (S. K. Eswara Moorthy / Luxembourg)
    10:45 – 11:00 Cryo correlative light microscopy and electron tomography illuminates lipids in Streptomyces cell division (Roman I.Koning / Leiden)
    11:00-11:15 Coffee Break
    Session V: Other Roads to CLEM II
    11:15 – 11:50 Acquisition of large datasets in the olfactory bulb of the zebrafish larvae with serial-block face scanning electron microscopy (Christel Genoud / Basel)
    11:50 – 12:25 Correlating fluorescence microscopy and electron tomography to visualize dynamic membrane ultrastructures (Wanda Kukulski / Heidelberg)
    12:25 – 12:45 X-ray Microscopy: illuminating the path from LM to 3D EM (Arno Merkle / Thornwood)
    12:45 – 13:00 Wrap-Up
    13:00 Lunch
    13:30 Departure Bus Transfer to Station or City
    14:00 – 15:40 Workshops (CAT, 3View, FIB-SEM, WetLab, Computing)
    15:50 Departure Bus Transfer to Station or City
  • Travel & Accomodation

    How to get to Ghent
    • Conference Center Het Pand, Onderbergen 1

    • VIB, Technologiepark 927

    The openings and keynote talks will be at the "Het Pand" conference center in the city center. The conference center is located next to the Sint-Michiels church.
    Program your navigation system with the following address.
    Conference Center Het Pand
    Onderbergen 1, 9000 Ghent

    The remaining event will take place directly at the Flanders Institute for Biotechnology (VIB).
    Use the following street address in your navigation system:
    Technologiepark 927, 9000 Ghent

    From Brussels there are three fast trains per hour to Ghent "Sint Pieters station". After arriving at Sint Pieters train station in Ghent you can take a cab or the tram number 1 to the Korenmarket to arrive in the city center.

    Brussels is the closest international airport. From Brussels there are regular train connections to Ghent.



    Recommended Hotels in walking distance to the Shuttle Bus:

    NH Gent Belfort

    Pick up point for the shuttle bus to the VIB is the so-called Belfrey of Ghent.


  • Ghent

    Ghent´s (celtic: Ganda) historic architecture remains intact and is remarkably well preserved and restored. Saint Bavo Cathedral with the Ghent Altarpiece, the belfry, the Gravensteen castle, and the splendid architecture along the old Graslei harbour are interesting highlights.The university hall Aula, the opera house and the main courthouse were built in the 19th century from Ghent's most famous architect, Louis Roelandt.

    Find more information:  Ghent: Tourist Office

  • Registration
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