| General | Laser Scanning Microscopy is a standard method in biomedical research. Excellent knowledge of its theory and application is a prerequisite for experimental success. This course provides detailed knowledge about point scanning confocal microscopes in respect to its assembly, features, and application in biomedical sciences. |
| Topics | • Principles of point scanning LSM • Beam paths and configurations of the LSM 700 • Variable Secondary Dichroic Beamsplitter (VSD) • Emission filters and avoiding Crosstalk • Image acquisition and optimization • Multi fluorescent imaging and channel linear unmixing • Acquisition of Lambda stacks and Linear unmixing • Basic image analysis and Colocalization • 3D image acquisition and reconstruction • System maintenance and care (SMT, ConfigTool, MTB) • Objective lenses selection for Laser Scanning Microscopy All hands on will be exercised on LSM 7-generation systems (LSM 700) using the ZEISS ZEN software. |
| Prerequisites | A basic knowledge in laser scanning microscopy is recommended. |
| Date | April 15 - 16, 2014 |
| Participants | max. 4 |
| Duration | 2 days, 9 am - 5:30 pm |
| Language | English |
| Course Fee | 1020,00 € for a two day course excl. tax; Course material and lunch are included. |
| Location | Carl-Zeiss-Promenade 10 07745 Jena Germany |
