Laser Scanning Microscopy

Microscopy Course

Laser Scanning Microscopy

LSM 700 - Biomedical Applications

  • Description
    General

    Laser Scanning Microscopy is a standard method in biomedical research. Excellent knowledge of its theory and application is a prerequisite for experimental success.

    This course provides detailed knowledge about point scanning confocal microscopes in respect to its assembly, features, and application in biomedical sciences.

    Topics • Principles of point scanning LSM
      • Beam paths and configurations of the LSM 700
      • Variable Secondary Dichroic Beamsplitter (VSD)
    • Emission filters and avoiding Crosstalk
    • Image acquisition and optimization
    • Multi fluorescent imaging and channel linear unmixing
    • Acquisition of Lambda stacks and Linear unmixing
    • Basic image analysis and Colocalization
    • 3D image acquisition and reconstruction
    • System maintenance and care (SMT, ConfigTool, MTB)
    • Objective lenses selection for Laser Scanning Microscopy

    All hands on will be exercised on LSM 7-generation systems (LSM 700) using the ZEISS ZEN software.
    Prerequisites A basic knowledge in laser scanning microscopy is recommended.
    Date April  15 - 16, 2014
    Participants max. 4
    Duration 2 days, 9 am - 5:30 pm
    Language English
    Course Fee 1020,00 € for a two day course excl. tax;
    Course material and lunch are included.
    Location Carl-Zeiss-Promenade 10
    07745 Jena
    Germany
  • Registration
    Invalid event id.

If you have further questions, please contact:
E-mail:
    courses .microscopy .de @zeiss .com

We use cookies on this site. Cookies are small text files that are stored on your computer by websites. Cookies are widely used and help to optimize the pages that you view. By using this site, you agree to their use. more