From live imaging to cleared samples @ Lightsheet 7

Life sciences research can put big demands on your imaging capability. It sometimes requires imaging of whole living model organisms, tissues and cells as they develop. Light sheet fluorescence microscopy (LSFM) with its unique illumination principle is ideal for fast and gentle imaging of such specimens. It enables the observation of living samples over extended periods of time — even days — with less phototoxicity than ever before. What’s more, you can employ this technique to image very large optically cleared specimens in toto, and with subcellular resolution.
This course provides detailed knowledge about LSFM and lots of hands-on @ Lightsheet 7.

Topics of the course

	Beam path & theory Lightsheet 7 Sample preparation Overview & assembly of sample holders Sample chamber building Change illumination and detection optics Single Side Illumination and Dual Side Illumination with Fusion options Multiview: Quick Setup, Manual Setup, and Processing with Fusion Incubation Online processing Tiling & Stitching in ZEN Processing tips in ZEN Blue (create an image subset, resample, channel shift, channel alignment) Processing using arivis Vision4D (stitching, channel shift in XYZ) Deconvolution Clearing hardware and imaging tips Customer interfaces and system maintenance & care
Prerequisites	High speed wired or wireless internet connection. Computer speakers, headphones or, a reliable telephone connection. Basic knowledge of Light Microscopy
Date	September 13 - 17, 2021
Duration	5 x 4 hour(s)
Participants	Max. 3
Language	English
Course Fees	3600 € (group discount possible)
Location	Training and Support Center Jena

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Venue

Training and Support Center Jena

Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany

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