From live imaging to cleared samples @ Lightsheet 7

Life sciences research can put big demands on your imaging capability. It sometimes requires imaging of whole living model organisms, tissues and cells as they develop. Light sheet fluorescence microscopy (LSFM) with its unique illumination principle is ideal for fast and gentle imaging of such specimens. It enables the observation of living samples over extended periods of time — even days — with less phototoxicity than ever before. What’s more, you can employ this technique to image very large optically cleared specimens in toto, and with subcellular resolution.
This course provides detailed knowledge about LSFM and lots of hands-on @ Lightsheet 7.

Topics of the course

 

  • Beam path & theory Lightsheet 7
  • Sample preparation
  • Overview & assembly of sample holders
  • Sample chamber building
  • Change illumination and detection optics
  • Single Side Illumination and Dual Side Illumination with Fusion options
  • Multiview: Quick Setup, Manual Setup, and Processing with Fusion
  • Incubation
  • Online processing
  • Tiling & Stitching in ZEN
  • Processing tips in ZEN Blue (create an image subset, resample, channel shift, channel alignment)
  • Processing using arivis Vision4D (stitching, channel shift in XYZ)
  • Deconvolution
  • Clearing hardware and imaging tips
  • Customer interfaces and system maintenance & care

 

Prerequisites

Basic knowledge of Light Microscopy

 

Date

September 13 - 17, 2021

 

Duration

5 day(s)

 

Participants

Max. 3

 

Language

English

 

Course Fees

3600 €

 

Location

Training and Support Center Jena, Germany

 

Request Course

Venue

Training and Support Center Jena

Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany