Advanced F-Techniques: FRAP & FRET

Advanced fluorescence techniques, commonly known as the F-techniques, measure the kinetics and the interactions of biomolecules with high sensitivity and spatiotemporal resolution.
This course provides detailed theoretical and practical knowledge about principles and applications of the most commonly used F-techniques: Fluorescence Recovery After Photo-bleaching (FRAP) & Förster Resonance Energy Transfer (FRET) on the LSM 9 family.

Topics of the course

 

  • FRAP (Fluorescence Recovery After Photobleaching)
  • FRET (Forster Resonance Energy Transfer), a technology to prove molecular interaction on the basis of (confocal) light microscopy
  • Theory of other Photomanipulation strategies (e.g. Photoconversion)

 

Learning Level

Advanced

 

Prerequisites

Fundamental knowledge about confocal laser scanning microscopy
ZEN Modules FRAP and FRET are recommended

 

Systems

LSM 9 family

 

Software

ZEN (blue edition)

 

Date

November 14 to 15, 2022
Registration until 2 weeks prior to the training

 

Duration

2 days (2 x 4 hours)

 

Location

Virtual classroom
(Required: reliable internet connection; computer speakers, headphones or a reliable telephone connection; TeamViewer)

 

Language

English

 

Course Fee

Per person: 900 € (plus VAT)

 

Participants

Max. 3

 

For additional information please send a request to courses .microscopy .de @zeiss .com.

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