Abstract:

Organoids are self-assembling, simplified model systems of organs grown in-vitro that have gained enormous interest for modelling tissue development and disease, as well as personalized medicine, drug screening and cell therapy. Despite considerable success in culturing physiologically relevant organoids, several challenges remain in quantifying data from these model systems, including major hurdles in optical imaging, sectioning, robust image analysis and storage of large data sets associated with 3D biology. Even though organoids hold the potential to usher in more predictable and accurate research, the challenges they present require practical solutions before their use can be applied on a large scale and their potential unlocked. In this talk, we will discuss a real-word case study of how ZEISS has overcome these difficulties in accurate 3D image capture and true 3D volumetric data analysis. We present a unified digital ecosystem through a combination of unique hardware capabilities, automated imaging workflows, and machine learning to make tomorrow’s scientific breakthroughs possible today. From image to results – ZEISS has connected the entire workflow for complex biological assays.

Presented by: Ryan Jacobs, Business Development Manager, Biotech/Pharma, ZEISS Research Microscopy Solutions

Abstract:

The incredible power of scanning electron microscopy (SEM) to provide high resolution structural insights in a wide range of biological samples is well appreciated. However, improvements in technological capabilities, correlative imaging, and sample management have enabled the expansion of these insights into even larger 2D areas as well as 3D volumes, transforming our understanding of cell biology. This talk will explore how ZEISS is using array tomography, scanning transmission electron microscopy (STEM), room temperature and cryo capable focused ion beam (FIB)-SEM, multi-modal imaging, and large area EM acquisition to reveal 3D cellular ultrastructure at ultra-high resolution. Join us to learn how you can use these assorted approaches to increase the area or volume you capture using SEM, explore examples of how these methods have revealed new cellular insights and expanded the scope of biological questions that can be addressed, and hear about the advantages provided by each approach in expanding your cellular research into 3-dimensions and beyond.

Presented by:  Aubrey Funke, Product Marketing Manager, Life Science EM and XRM, ZEISS Research Microscopy Solutions

Abstract:

Live cell imaging doesn’t have to be hard. It is a powerful tool in understanding development, cell to cell interactions and evaluating drug efficacy, but accomplishing stable long-term imaging poses many challenges beyond just controlling environmental conditions. Advances in hardware have overcome hurdles in focus drift, well plate calibration, high resolution timelapse imaging, and lens adaption for vessel material, bottom thickness and media refractive indices. ZEISS AI Sample Finder, Definite Focus 3, autocorrect objectives and fully integrated incubation, allow you to spend less face time with the microscope, enabling more freedom and efficiency in your work. Combined with ZEN Bio Apps one-click analysis and ZEN Connect project documentation and navigation and we offer a complete solution to support your research. Power on the microscope, place your sample on the stage and the rest you can do from your desk, a different campus or home. Whether you are looking at long term live cell imaging, fixed cell screening, or whole tissue imaging, each of these advancements work together to make microscopy easier and more reliable by reducing the burden on the user. Join us to learn about the latest advances in live cell imaging and see how the ZEISS ecosystem saves you time and money. Achieve high quality live cell imaging, the easy way.

Presented by:  Elise Shumsky, Head of Product Application Sales Specialists, ZEISS Research Microscopy Solutions