ZOYC

ZEISS LSM 980 and Axioscan 7 Workshop

Microscopy Solutions for Spatial Omics

Hosted by Scott Fraser at the University of Southern California, Michelson Translational Imaging Center
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Workshop

Microscopy Solutions for Spatial Omics – High throughput screening, high-resolution 3D imaging, and data analysis

Our ZEISS On Your Campus (ZOYC) roadshow is touring the country with our ZEISS Axioscan 7 and ZEISS LSM 980. This special workshop will focus on applying our 2D and 3D spectral multiplexing technology to a wide range of spatial omics applications. The workshop will consist of in-person demonstrations and a one hour live presentation (in-person and via webinar broadcast). We will introduce the technology and applications and will also include a discussion on our ZEISS analysis software solutions relevant to this application space. We will have two hour demo time slots available, hosted by Scott Fraser at the University of Southern California, Michelson Translational Imaging Center from October 17-21, 2022. To register for the webinar and/or sign-up for a demonstration, use the form below. We look forward to your participation.
 

In this workshop, you will learn:

  • How to apply our 3D spectral multiplexing confocal technology to a wide range of spatial omics applications
  • How you can increase your throughput for 2D spatial omics applications without compromising image quality with the Axioscan 7
  • New tools such as LSM Plus to further increase resolution and sensitivity and acquire the signature of 10 or more markers in a single scan
  • Connect your high throughput and high-resolution spatial omics workflows with ease using ZEN Connect on the Axioscan 7 and the LSM 980
  • A number of our ZEISS analysis software solutions relevant to this application space

Presentation

This is a hybrid presentation on October 18th, 2022 from 10am-11am PDT, hosted at the University of Southern California campus at the Michelson Translational Imaging Center MCB-102 and virutally on GoToWebinar for those who cannot attend in person. Webinar details will be provided via email after you register for the event below. Coffee and refreshments provided. 

In-Person Product Demonstrations with ZEISS Tissue Samples

We will be hosting 2 hour demo time slots available at the University of Southern California October 17-21, 2022. To sign-up for a demonstration, use the form below. We look forward to your participation.

LSM 980 Demonstrations:
Michelson Translational Imaging Center MCB-451
October 17-21, 2022 | 10am-12pm PDT, 1-3pm PDT, 3-5pm PDT


Axioscan 7 Demonstrations:
*If you would like a demonstration on your own samples, slides and details must be dropped off by 10/11
Michelson Translational Imaging Center MCB-405
October 17, 2022 | 1-3pm PDT, 3-5pm PDT
October 18-20, 2022 | 10am-12pm PDT, 1-3pm PDT, 3-5pm PDT
October 21, 2022 | 10am-12pm PDT

Presented By

Rick Marolt

Your Local Account Manager

Rick grew up in the Southern California area and completed his BS in Marine Biology at California State University, Long Beach. His research project was to determine marine grazing rates of parasitic copepods based on differing eco environments. He also obtained a minor in Geology.

Prior to joining Zeiss, he was a research technician at Chevron working in the Oil Exploration Biostratigraphy group using siliceous microfossils as timeline markers for offshore exploration. During this time, he used light and electron microscopy to identify species of importance in creating these appearance/extinction timelines.

Presented By

Sebastian Barreto Ortiz, Ph.D.

Automated Product Application Sales Specialist

Sebastian Barreto Ortiz is currently an Automated Product Application Sales Specialist focused on the ZEISS Celldiscoverer 7 and the Axioscan 7 systems. Sebastian earned his Ph.D. from the Johns Hopkins University in Chemical and Biomolecular engineering, where he worked in vascular tissue engineering fabricating small artificial blood vessels in vitro. Here he developed his love for microscopy, which was the main analysis technique to study the organization of the microvessels he generated

Presented By

Geert Vreede, Ph.D.

3D Product Application Sales Specialist

Prior to joining ZEISS, Geert did his Ph.D. work at the University of California Berkeley in the Molecular and Cell Biology department. He completed his thesis research in the lab of Dr. David Bilder, using extensive confocal and widefield microscopy techniques to study novel mechanisms of epithelial ligand-receptor interactions and tumor suppressor genes in Drosophila. His enthusiasm for microscopy started during undergrad in the lab of Prof. Sander Van Den Heuvel at Utrecht University in The Netherlands, performing live imaging of cell division in C. elegans.

Geert welcomes the opportunity to speak with you to learn more about your current research requirements, imaging projects and challenges, and share with you some of the recent, unique enhancements to ZEISS Microscopy solutions.

Presented By

Kevin O'Keefe

Senior Software Sales Biotech Pharma

Prior to joining ZEISS Kevin O’Keefe completed his Ph.D. at the University at Albany, SUNY, where he gained extensive experience in widefield, confocal microscopy and image analysis with a focus on tissue multiplexing following injury in the mouse submandibular salivary gland. After graduating Kevin started his career at ZEISS as a Product Application Sales Specialist and covered NY, NJ and PA working with ZEISS hardware including advanced 3D imaging systems. Recently, Kevin has joined the software sales team as the Senior Software Sales Representative with a concentration in biotech and pharma. He focuses on using the ZEISS toolkit for image analysis including ZEN, APEER and Arivis to help translate images into actionable data.

Presented By

Torey Arnold, Ph.D.

Local Biotech/Pharma Account Manager

My name is Torey and I go by all gender pronouns. I am a biologist at heart and studied cell-cell interactions and mechanics for my Ph.D. work at the University of Michigan and Post-Docs at University of Michigan and Boston College. I love using advanced microscopy techniques to understand how cells function and impact our daily lives. It would be my honor to learn more about your science goals and to provide information and microscopy solutions.

Abstract

In the past 5 years, the life sciences community has witnessed the progression of the genomics and proteomics world into the spatial domain. This was in large part due to a strong desire to not only know which genes were being turned on and which proteins were being expressed, but also precisely where in a much larger context. This concept, often referred to as actionable biology, is leading to a much deeper understanding of diseases and genetic disorders and is transforming the developments of treatments, cures, and drug discoveries. Certainly, the spatial concept easily lends itself to microscopy methods, and we have seen the release of many powerful instruments on the market dedicated to spatial omics applications.

Initially, this work was almost exclusively being done on very thin tissue sections or monolayers of cells, and therefore utilized only 2D imaging and widefield microscopy. However, biology is inherently 3D, and the desire to resolve information in all three dimensions is increasing. Furthermore, we see researchers pushing these methods into even thicker tissue samples, obviating the need for an optical sectioning microscopy method to accomplish the imaging goals. Advances in the detection range of the LSM 980, coupled with new tools such as LSM Plus, further increase resolution and sensitivity and allow acquisition of 10 or more markers in a single scan. Combining the LSM 980 with high throughput whole slide imaging on the Axioscan, allows for high-resolution imaging in 3D without losing context. These tools provide the spectral and application flexibility needed to meet the demands of the growing number of biomarkers pushing out into the NIR wavelength range including smFISH and RNAscope®. Uncover the details of cell-to-cell interactions, cellular phenotyping and transcriptomics, without compromising context. Take it to the next level with our suite of software analysis utilizing AI and deep learning to segment and quantify the spatial relationships within your data.

Register

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Workshop Location

University of Southern California

Michelson Translational Imaging Center 1002 West Childs Way 90089 Los Angeles United States