Microscopy Events

ZEISS at Neuroscience 2017

November 12-15, 2017 - Washington, D.C. USA, booth #2923

Ultraresolution Imaging


Accomplish ultra-resolution imaging of neural connections and intracellular structures with electron microscopy. Learn about our solutions for 3D reconstruction of large volumes or find out about the fastest scanning electron microscope available – image up to centimeters of tissue.

Imaging large, cleared samples

With the only light sheet microscope on the market with lenses specific for cleared tissues and revolutionary Airyscan-equipped confocals, ZEISS is uniquely positioned to image large, cleared samples. Visit us at Neuroscience to see ZEISS Lightsheet Z.1 in action.

Better confocal imaging


With Airyscan, and now with Fast technology, ZEISS confocals are revolutionizing imaging. With better signal-to-noise, superresolution with any fluorophore, and fast imaging, find out what you’re missing with your current confocal system. Test drive one at Neuroscience.

 

Book a demo

Test drive our equipment and learn how ZEISS can help you further your research.

Electron Microscopes
GeminiSEM 300 FE-SEM Get sub-nanometer resolution and high detection efficiency, even in variable pressure mode. Book a demo
Confocal & 3D Imaging Systems
Lightsheet Z.1 light sheet imaging system Test drive how light sheet microscopy pushes the boundaries of 3D imaging of large samples. Book a demo
LSM 800 with Airyscan confocal microscope Benefit from Airyscan technology in a confocal designed for individual labs. Book a demo
LSM 880 with Airyscan confocal microscope With the unparalleled combination of signal-to-noise, speed and resolution, experience the new standard in confocal imaging. Book a demo
Automated Imaging Systems
Axio Scan.Z1 digital slide scanning system Increase your productivity. Load up Axio Scan.Z1 with up to 100 slides and image with a single push of a button. Book a demo
Celldiscoverer 7 Record setting optics and full automation simplifies your live cell imaging like never before. Book a demo

 

Attend a Seminar

  • Agenda
    Date & Time Title
    Sunday, November 12, 2017
    12:00 pm - 1:00 pm
    Airyscan Microscopy a Promising Tool for High Resolution Live Cell Imaging
    Register
    Monday, November 13, 2017
    12:00 pm - 1:00 pm
    Charge Compensation by Focal Gas Injection Enables High-performance Serial Block-Face SEM on Non-conductive Samples
    Register
    Tuesday, November 14, 2017
    12:00 pm - 1:00 pm
    Biological Applications of X-Ray Microscopy and Correlative XRM - FIB-SEM Imaging
    Register
    Wednesday, November 15, 2017
    12:00 pm - 1:00 pm
    ZEISS MultiSEM: The World’s Fastest Scanning Electron Microscope
    Register
  • Abstracts

    Sunday, November 12, 2017, 12:00 pm - 1:00 pm
    Airyscan Microscopy a Promising Tool for High Resolution Live Cell Imaging

    Kseniya Korobchevskaya1, B. Christoffer Lagerholm2, Huw Colin-York3, and Marco Fritzsche1,3
    1 Kennedy Institute for Rheumatology, Roosevelt Drive, University of Oxford, Oxford OX3 7LF Oxford, United Kingdom
    2
    Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, OX3 9DS Oxford, United Kingdom
    3 MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, OX3 9DS Oxford, United Kingdom

    Confocal microscopy is a broadly used tool for fluorescent imaging thanks to its good resolution and optical sectioning abilities. However, the best resolution performance requires using smallest possible pinhole diameter, which is seldom possible due to insufficient brightness (low signal-to-noise ratio) of biological specimens. The Airyscan technology was developed to overcome these issues, by combining compound detector array, deconvolution and the pixel-reassignment principles. To quantify the improvement we performed a detailed study by comparing the Airyscan with conventional confocal imaging. We have shown that the Airyscan approach provides resolution as high as confocal at 0.2 Airy Units but with significantly higher signal-to-noise ratio, but without increasing the excitation power and acquisition time, which makes it a great candidate for live-cell imaging.

    Register

    Monday, November 13, 2017, 12:00 pm - 1:00 pm
    Charge Compensation by Focal Gas Injection Enables High-performance Serial Block-Face SEM on Non-conductive Samples

    Matthias Haberl, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA, USA

    Serial block-face scanning electron microscopy (SBEM) is a powerful method delivering 3D brain volumes and propelling research into mapping the fine-scale connectome. In this session, we describe a new approach for completely eliminating sample charging while acquiring SBEM data at high vacuum. We will provide examples of high-resolution imaging of samples prone to charging, showing how our solution improves resolution and signal-to-noise without increasing data acquisition time. This advance will significantly expand the use of SBEM, in general, and allow for use of previously challenging samples. Since signal-to-noise is also enhanced, deep learning based segmentation becomes easier and larger connectomes can be assembled.

    Register

    Tuesday, November 14, 2017, 12:00 pm - 1:00 pm
    Biological Applications of X-Ray Microscopy and Correlative XRM - FIB-SEM Imaging

    James A.J. Fitzpatrick, Ph.D., Director, Center for Cellular Imaging
    Associate Professor, Cell Biology & Physiology, Neuroscience and Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA

    X-Ray Microscopy (XRM) is a relatively new technique that combines the geometric magnification of traditional micro-CT with the optical magnification of light microscopy. The ZEISS Versa XRM system allows an unprecedented view inside samples varying in size from the mesoscale (cm) to the microscale (um) at consistently sub-micron image resolutions. This webinar will focus on the biological applications of X-Ray microscopy, covering the imaging of calcified structures (such as bone) to soft tissues such as the intervertebral disc to visualizing blood vessel using vascular tracing agents. The basics of sample preparation will be covered as well as the pros and cons of different imaging conditions. Finally, a sneak view will be provided into using XRM to spatially target, in three-dimensions, tissue specific structures in a whole organism for 3D ultrastructural imaging using Focused Ion Beam - Scanning Electron Microscopy (FIB-SEM) using the ATLAS 5 Correlative Workspace.

    Register

    Wednesday, November 15, 12:00 pm - 1:00 pm
    ZEISS MultiSEM: The World’s Fastest Scanning Electron Microscope

    Dr. Stephan Nickell, Business Development mSEM, Carl Zeiss Microscopy GmbH

    Studies in Connectomics require the imaging of structures spanning several orders of magnitude in size. While conventional light microscopy is suitable for mapping large areas, it has its limitations in distinguishing smallest features at the subcellular level. Conversely, electron microscopy can resolve nanometer-sized details, but fails to provide the necessary throughput to image relevant volumes in reasonable time frames. Multi-beam scanning electron microscopy can overcome this gap by providing high imaging throughput at nanometer resolution and is therefore the method of choice for obtaining dense reconstructions in Connectomics.

    ZEISS MultiSEM employs up to 91 parallel electron beams in a single instrument to increase the field of view while maintaining the high spatial resolution of scanning electron microscopes. In the presentation we will explain the mSEM technology, show the application workflow in Connectomics and discuss challenges in data processing.

    Register

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