Visit booth #511 and discover solutions from ZEISS for your cellular and molecular applications. Test drive instruments in person.
- Attend our seminars to learn from experts about the microscopy innovations and advancements
- Request a demonstration on our system to experience first hands the benefits of a ZEISS system
- Talk to our experts about your complicated applications to find the perfect solution
On December 4th, ZEISS will open a new chapter in superresolution microscopy. Visit the ZEISS booth at ASCB and be among the first to experience it.
ZEISS Elyra 7 with Lattice SIM
New from ZEISS: experience the fastest, most versatile Structured Illumination system ever available. Elyra 7 allows you to image faster and longer without compromising on resolution.
arivis InViewR 3D Visualization
Dive deep into your 3D image data in virtual reality and view your samples as never before. Use your hands to move, rotate, scale, and shape your digital image data.
ZEISS Celldiscoverer 7
Automated boxed microscope with the image quality of an inverted microscope that is capable of calibrating itself and focusing its optics to automatically detect and focus onto your samples.
ZEISS Digital Classroom
Promote an interactive learning environment by connecting microscopes to tablets or phones via ZEISS Labscope. Monitor and project what students are seeing on digital devices and engage with them better.
ZEISS LSM 880
Confocal microscope to image a variety of samples from thick to thin. Capture the smallest structures, detect the faintest signals, track the fastest process – or do it all at once.
ZEISS Cryo Workflows
Come talk to a ZEISS expert about high-content cryo microscopy solutions. Combine Cryo AiryScan and Cryo EM to see your sample like you’ve never been able to before.
12:00 PM - 12:30 PM
Celldiscoverer 7 and 3D-Cell Culture - High Resolution meets Screening
Dr. René Buschow
Max Planck Institute for Molecular Genetics, Berlin, Germany
The use of three-dimensional cell culture systems gain more and more attention in the field of developmental biology, personalized medicine and cancer research, as well as general cell biology. We developed an experimental pipeline that enables us to monitor and quantify the viabilities of 3D cell systems under live cell conditions.
Using a low magnification, i.e. large object fields, ultra-low attachment tissue cultures were imaged in 384-well plates in a fully automated mode. Downstream, the image data was transferred into an automated analysis and archiving pipeline (Columbus, Perkin Elmer).
Prerequisite for live cell imaging in 384-well-formats is a stable environmental control as well as a light efficient microscope to reduce exposure times, phototoxicity, and guarantees an adequate throughput. Further, our set up finally required a fluorescence 3D imaging routine for evaluation of fluorescence reporters under live cell conditions (e.g. marker, viability staining, or drug availability).
The hardware to encounter these experimental tasks is all combined within the novel microscopy platform Celldiscoverer 7 (by Zeiss). This fully automated research imaging system enables a broad range of magnifications (2.5x-100x), large working distances and full environmental control, making it the ideal instrument for live cell 3D-systems. Further, automated focus strategies and outstanding Autocorr objectives ensure optimal imaging and constant photon yields for a reliable quantification of fluorescence signals in combination with modern 3D-deconvolution algorithms.
We use ZEN 2.5 as imaging and deconvolution software, for initial processing steps and established a data transfer pipeline. The final image analysis and archiving is performed using PerkinElmers Columbus platform.
The combination of the superior optics and imaging flexibility of the Celldiscoverer 7 plus the analysis platform Columbus (Perkin Elmer) enables the hybrid between modern High Content Microscopy with the flexibility required in academic research.
12:30 PM - 1:00 PM
Structured Illumination Microscopy (SIM) with Two-Dimensional Illumination Patterns
Rainer Heintzmann, Ronny Förster, Christian Karras
Institute of Physical Chemistry, Friedrich-Schiller-Universität and Leibniz Institute of Photonic Technology, Jena, Germany
3D-SIM is often a preferred mode of minimally invasive imaging subcellular structures at high resolution. However, it sometimes struggles to deal with densely stained thick samples and with fast sample movements due to the requirement to obtain many, typically 15, raw images for a single high-resolution frame.
Here we analyze the performance of different illumination patterns, including linear gratings and two-dimensional patterns generated incoherently (i.e. sequentially or dithered as in lattice light-sheet microscopy) or by coherent superposition (e.g. in SIM or coherent lattice light-sheet microscopy). Imaging results in various modes are compared in terms of the recently introduced noise-normalized optical transfer function OTFnn. Incoherent superposition is found to be inherently inferior to coherent illumination strategies.
We present a detailed theoretical comparison as well as experimental results on living cells.
1:00 PM - 1:45 PM
Visualizing Intracellular Organelle and Cytoskeletal Interactions at Nanoscale Resolution on Millisecond Time Scales
Dr. Eric Betzig
Janelia Research Campus, Virginia, USA
In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long duration imaging of the intracellular environment at high spatiotemporal resolution and low background. We developed a grazing incidence form of structured illumination microscopy (GI-SIM) that images dynamic events near the basal cell cortex at 97 nm resolution and 266 frames/sec over thousands of time points. We employed the technology in multiple colors to characterize dynamic interactions between organelles and the cytoskeleton, including ER remodeling via interactions with other organelles and/or microtubules, and ER-mediated mitochondrial fission and fusion.
Have a question? Fill out the form to get in touch with us.