Seminars at ASCB | EMBO 2018

December 9, 2018 – 1:00 PM - 1:45 PM | Theater 2, Learning Center

Visualizing Intracellular Organelle and Cytoskeletal Interactions at Nanoscale Resolution on Millisecond Time Scales

Dr. Eric Betzig
Janelia Research Campus, Virginia, USA

In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long duration imaging of the intracellular environment at high spatiotemporal resolution and low background. We developed a grazing incidence form of structured illumination microscopy (GI-SIM) that images dynamic events near the basal cell cortex at 97 nm resolution and 266 frames/sec over thousands of time points. We employed the technology in multiple colors to characterize dynamic interactions between organelles and the cytoskeleton, including ER remodeling via interactions with other organelles and/or microtubules, and ER-mediated mitochondrial fission and fusion.