Seminars at Neuroscience 2018

Sunday, November 4, 2018 – 12 PM - 1 PM

Part 1:
Resolving Brain Structures and Signaling in vivo with ZEISS Airyscan

Joseph Huff, Business Sector Life Sciences, Carl Zeiss Microscopy GmbH, Jena, Germany

Fluorescence microscopy allows for researchers to study the structure and function of the brain in both fixed samples and in vivo. Laser scanning microscopy, confocal and multi-photon, serve as the standard imaging approaches for imaging into scattering samples. However, due to the light scattering properties brain tissue, LSM suffers in both depth penetration and resolution. By combining the unique ZEISS Airyscan detection concept with multiphoton excitation, the resolution and signal-to-noise benefits of Airyscan can be extended to deeper layers of the cortex (2-3x deeper than traditional confocal). In addition, combining Airyscan with GRIN lens technology enables increased resolution and signal-to-noise while imaging other regions of the brain that are unreachable traditional in vivo microscopy.

Part 2:
Simultaneous Monitoring and Manipulation of Neural Circuit Dynamics in Freely Behaving Mice and Co-Registration of Enhanced Anatomical Detail with ZEISS Airyscan Head Fixed Data

Dr. Shanna Resendez, Regional Manager - Field Scientific Consultant, Inscopix Inc, Palo Alto, CA

The development of Ca2+ indicators for visualizing dynamic cellular activity has revolutionized systems neuroscience. However, due to the size and rigidity of traditional microscopy systems, the use of these indicators for the study of brain function has been limited to head fixed behaviors or anesthetized animal preparations. To circumvent this limitation, we have developed miniature lightweight microscopy systems (nVista and nVoke) that allow for cellular-resolution imaging and optogenetic manipulation in freely behaving animals. Our miniature microscopy systems have been optimized for paired use with chronically implanted GRIN lenses of various lengths enabling optical access to cortical and subcortical brain regions greatly expanding the types of behaviors that can be decoded, from naturalistic social behaviors in mice to complex cognitive processes in primates. Subsequent developments at Inscopix to enable co-registration of ZEISS Airyscan data with Inscopix’s large-scale neural activity data in freely moving animals represents a long-sought marriage of two cornerstone imaging modalities. Integrating subcellular and structural detail with functional neural network data can provide exciting new insights into brain function and dysfunction.