Seminars at Neuroscience 2018

Tuesday, November 6, 2018 – 12 PM - 1 PM

Cryo Light and Electron Microscopy: High Content - High Flexibility - High Resolution

Dr. Robert Kirmse, Carl Zeiss Microscopy GmbH, Germany

The investigation of vitrified biological specimens enables the visualization of cellular ultrastructure in a near-to-native fully hydrated state, unadulterated by harmful preparation methods. Utilizing Light and Electron Microscopy in combination is the next step for cryo microscopy workflows. ZEISS’ unique technologies with Airyscan and Crossbeam allow the analysis of functional and structural information under cryo conditions at high resolution, providing invaluable data for every step.

In this seminar, we will present two unique cryo imaging modalities and discuss their impact on cryo correlative workflows. First, we present confocal cryo fluorescence microscopy, utilizing the unique ZEISS Airyscan detector to improved signal to noise ratio (SNR) and resolution. Second, we present how large volume imaging using focused ion beam scanning electron microscopy (FIB/SEM) is offering new insights into vitrified specimens. Ultimately, both technologies are combined into a correlative workflow that offers full flexibility for each step providing high-resolution data and results in lamellas for cryo electron tomography.