ZEISS Talk at Neuroscience 2019

Sunday, October 20, 2019 – 12:00 pm - 1:00 pm

Technical approaches and advancements in imaging large volumes by serial electron microscopy
Gregg Wildenberg, PhD, Research Scientist, The University of Chicago/Argonne National Laboratory, Chicago, USA

Presently, many serial electron microscopy based connectomics projects are limited to small volumes that only capture pieces of neurons. A major goal in the field of connectomics is to routinely image large volumes of brain that capture and permit the analysis of large populations of neurons and the connections they make. Approaches that leverage the versatility of a single beam SEM to collect and analyze all cortical layers of the visual cortex of mouse and primate at different resolutions will be described in detail. Further, results from recent advances in multi-beam SEM (i.e. MultiSEM) imaging will be revealed as it enables fast imaging of large volumes at the resolution needed for complete circuit reconstruction.

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