ZEISS Talk at Neuroscience 2019

Monday, October 21, 2019 – 12:00 pm - 1:00 pm

Resolving brain structures and signaling in vivo with Airyscan 2
Joseph Huff, Business Sector Life Sciences, Carl Zeiss Microscopy GmbH, Jena, Germany

Fluorescence microscopy allows for researchers to study the structure and function of the brain in both fixed samples and in vivo. Laser scanning microscopy, confocal and multi-photon, serve as the standard imaging approaches for imaging into scattering samples. However, due to the light scattering properties brain tissue, LSM suffers in both depth penetration and resolution. By combining the unique Airyscan detection concept with multiphoton excitation, the resolution and signal-to-noise benefits of Airyscan can be extended to deeper layers of the cortex (2-3x deeper than traditional confocal). In addition, combining Airyscan with GRIN lens technology enables increased resolution and signal-to-noise while imaging other regions of the brain that are unreachable traditional in vivo microscopy. The combination of Airyscan imaging through a GRIN lens can also be correlated with freely behaving imaging to provide additional context to the data.
Joseph is part of the business sector life science group for ZEISS research microscopy systems focussing on laser scanning microscopy systems. Joseph’s function within the business sector can be described as both defining what comes next for the ZEISS laser scanning microscopy portfolio as well as building market-oriented value propositions for the current ZEISS LSM portfolio.

Registration across in vivo neuronal imaging modalities: Inscopix miniature microscopes to ZEISS high resolution Airyscan

Alice Stamatakis, PhD, Senior Lead Scientist, Inscopix Inc, Palo Alto, CA
Alice Stamatakis received her PhD in Neurobiology from the University of North Carolina where she studied the neural circuitry underlying motivated behaviors. Alice joined Inscopix in 2014 and is a Senior Lead Scientist driving product and application development.

We have developed nVista™ and nVoke™ miniature microscopy systems for cellular-resolution imaging and optogenetic manipulation in freely behaving animals. They have been optimized for use with GRIN lenses of various lengths enabling optical access to cortical and subcortical brain regions to greatly expand the types of behaviors that can be decoded, from naturalistic social behaviors in mice to complex cognitive processes in primates. To expand upon these applications, we have developed methodology to register the exact same neurons imaged with the Inscopix miniature microscopes to neurons imaged with a Zeiss Airyscan confocal microscope. The ability to register data from freely moving imaging experiments to data from high resolution confocal imaging will begin to provide crucial links between activity dynamics and anatomical, molecular, and/or connectivity profiles of distinct neuronal populations. We have developed hardware that enables recording from the exact same focal plane between the two modalities and analysis routines to correct for the different scale, rotation, and elastic deformations between the images. Using this solution, we have demonstrated the feasibility of identifying the same GCaMP neurons imaged in the mPFC with the nVista and Airyscan microscopes. Further, we are able to image a red static indicator with the Airyscan, and identify a subset of contralaterally-projecting mPFC neurons. Integrating functional network activity with structural details enables exciting new insights into brain health and disease.

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