In vivo Multiphoton Fluorescence Correlation Spectroscopy to Quantify Cerebral Blood Flow with High Spatiotemporal Resolution

Chris Richards, Ph.D.

University of Kentucky

Dr. Richards serves as the director of the Light Microscopy Facility at the University of Kentucky and is an associate professor in the Department of Chemistry. His research focuses on the development of ensemble and single-molecule fluorescence spectroscopy techniques for the study of complex biological systems. He has recently applied this approach to study substance-use disorders and the development of therapeutic delivery platforms.

Dr. Richards received his bachelor’s degree in chemistry from the University of Maine and his PhD in physical chemistry from the Georgia Institute of Technology under Robert Dickson. He was a postdoctoral researcher at Caltech, where he was also a postdoctoral Beckman Scholar.


Cerebral blood flow (CBF) measurements provide critical information about physiological and pathological processes within the central nervous system (CNS). The complex microvascular network plays a fundamental role within the CNS, where neuronal activity regulates the flow of nutrients. Understanding blood flow dynamics with high spatial and temporal resolution is essential to understanding the role of vascular dysfunction in a variety of pathological processes. Using multiphoton in vivo fluorescence correlation spectroscopy (FCS), blood flow rates can be determined at individual pixels with sub-micron resolution. Scanning the excitation beam using FCS provides pixel-by-pixel mapping of flow rates with sub-vessel resolution across capillaries up to 300 µm deep in the brains of mice. This webinar by Dr. Chris Richards will introduce in vivo FCS to map blood flow rates with sub-vessel resolution and monitor dynamic changes in flow velocity in the CNS of mice in response to changes in the animal’s physiological condition.