Did you know that traditional imaging techniques can influence the behaviour of specimens due to phototoxicity, thus affecting the integrity of the results? In order to best understand the world around us it is necessary to observe microscopic specimens in as natural a state as possible. This requires a transition from imaging fixed to live specimens and expanding from 2D to 3D model organisms.
The drive towards live-cell imaging over long timeframes and at high volume speeds, while maintaining sub-cellular resolution, brings new challenges. Challenges which can be addressed by utilizing lattice light-sheet technology. Traditionally utilized live cell imaging techniques such as classical widefield, confocal and spinning disk methods expose the sample to light both at the focal plane of interest and above and below it. With light-sheet microscopy light exposure is minimized by illuminating only the focal plane of interest. Lattice light-sheets are long thin light-sheets which minimize phototoxicity, improve signal to noise and deliver subcellular resolution. With the gentleness of lattice light-sheet microscopy it is possible to capture dynamics at previously unreachable combinations of acquisition speed and resolution over hours and even days.
This talk will describe how the ZEISS Lattice Lighsheet 7 makes long-term volumetric imaging of living cells with subcellular resolution possible without having to change your standard sample preparation protocols to accommodate the instrument. With automatic alignment and easy acquisition workflows, lattice light-sheet imaging is now as accessible as using a standard inverted microscope.
Join us for this webinar to learn how ZEISS Lattice Lightsheet 7 allows you to discover the subcellular dynamics of life.