Our ZEISS On Your Campus (ZOYC) roadshow is touring the country with our ZEISS Axioscan 7 and ZEISS LSM 980. This special workshop will focus on applying our 2D and 3D spectral multiplexing technology to a wide range of spatial omics applications. The workshop will consist of in-person demonstrations and a one hour live presentation (in-person and via webinar broadcast). We will introduce the technology and applications and will also include a discussion on our ZEISS analysis software solutions relevant to this application space. We will be hosting two hour demo time slots available at the University of North Carolina Neuroscience Microscopy Core from August 22-25, 2022. To register for the webinar and/or sign-up for a demonstration, use the form below. We look forward to your participation.
Due to COVID-19 safety precautions, we can accommodate up to 3 people per demo session. Additional colleagues will need to sign up for a separate session or join the demonstration virtually. Space is limited.
Room 3116, Mary Ellen Jones Building, University of North Carolina
August 22-25, 2022 | 10-12 am EDT, 1-3 pm EDT, 3-5pm EDT
August 22-23, 2022 | 10-12 am EDT, 1-3 pm EDT, 3-5pm EDT
August 24, 2022 | 10-12 am EDT
In this workshop, you will learn:
- How to apply our 3D spectral multiplexing confocal technology to a wide range of spatial omics applications
- How you can increase your throughput for 2D spatial omics applications without compromising image quality with the Axioscan 7
- New tools such as LSM Plus to further increase resolution and sensitivity and acquire the signature of 10 or more markers in a single scan
- Connect your high throughput and high-resolution spatial omics workflows with ease using ZEN Connect on the Axioscan 7 and the LSM 980
- A number of our ZEISS analysis software solutions relevant to this application space
In the past 5 years, the life sciences community has witnessed the progression of the genomics and proteomics world into the spatial domain. This was in large part due to a strong desire to not only know which genes were being turned on and which proteins were being expressed, but also precisely where in a much larger context. This concept, often referred to as actionable biology, is leading to a much deeper understanding of diseases and genetic disorders and is transforming the developments of treatments, cures, and drug discoveries. Certainly, the spatial concept easily lends itself to microscopy methods, and we have seen the release of many powerful instruments on the market dedicated to spatial omics applications.
Initially, this work was almost exclusively being done on very thin tissue sections or monolayers of cells, and therefore utilized only 2D imaging and widefield microscopy. However, biology is inherently 3D, and the desire to resolve information in all three dimensions is increasing. Furthermore, we see researchers pushing these methods into even thicker tissue samples, obviating the need for an optical sectioning microscopy method to accomplish the imaging goals. Advances in the detection range of the LSM 980, coupled with new tools such as LSM Plus, further increase resolution and sensitivity and allow acquisition of 10 or more markers in a single scan. Combining the LSM 980 with high throughput whole slide imaging on the Axioscan, allows for high-resolution imaging in 3D without losing context. These tools provide the spectral and application flexibility needed to meet the demands of the growing number of biomarkers pushing out into the NIR wavelength range including smFISH and RNAscope®. Uncover the details of cell-to-cell interactions, cellular phenotyping and transcriptomics, without compromising context. Take it to the next level with our suite of software analysis utilizing AI and deep learning to segment and quantify the spatial relationships within your data.
James completed his undergraduate degree at McGill University and went on to receive his Ph.D. at University of Toronto in Biochemistry. He stumbled into to the world of microscopy through luck by joining Dr. Christopher Yip’s lab as one of the first graduate students in Canada integrating Atomic Force Microscopy (AFM) with Confocal. They worked on correlative studies with various biological model systems. He then went on to spend three years in postdoctoral studies in Alzheimer’s drug development, also at University of Toronto. He then made the switch to industry joining Bruker Nano Surface as a Bio Sales Application Engineer based in Philadelphia, PA. At Bruker he transitioned into several positions including lead Bio Application Test Engineer for the development of Bruker’s next generation Bio AFM base in Santa Barbara, CA. His most recent position in Industry was with Bitplane/Imaris where he was their southeast regional sales engineer supporting 3D/4D image data visualization and analysis software in metro Washington, DC area. Over the years, he has enjoyed the interactions with his fellow scientists/customers the most, as a part of his day-to-day job, and joins the ZEISS team where he will have plenty of those wonderful interactions with customers and colleagues.
Prior to joining Zeiss, Andy was the director of the Purdue University Imaging Facility for 6 years where he deciphered researchers' imaging needs, managed several high-end microscopes, instructed users, and ensured their understanding and execution of imaging experiments and purchased a wide variety of imaging modalities. He earned his PhD in Medical Microbiology and Immunology from Texas Tech University Health Science Center. During his research years, he studied and measured the ability of quorum sensing P. aeruginosa mutants to form both in vivo and in vitro biofilms using confocal and widefield microscopes. His current passion is to provide simple yet powerful microscope systems to our clients helping them effectively push the boundary of science.
Prior to joining ZEISS Kevin O’Keefe completed his Ph.D. at the University at Albany, SUNY, where he gained extensive experience in widefield, confocal microscopy and image analysis with a focus on tissue multiplexing following injury in the mouse submandibular salivary gland. After graduating Kevin started his career at ZEISS as a Product Application Sales Specialist and covered NY, NJ and PA working with ZEISS hardware including advanced 3D imaging systems. Recently, Kevin has joined the software sales team as the Senior Software Sales Representative with a concentration in biotech and pharma. He focuses on using the ZEISS toolkit for image analysis including ZEN, APEER and Arivis to help translate images into actionable data.
Garrick grew up in Ohio and completed his BS at the University of Mount Union in Chemistry. His research project was on nanoparticles which utilized SEM for analysis. Garrick went on to complete his MS at the University of North Carolina Wilmington where he built a spectral imaging instrument.
Prior to coming to ZEISS, Garrick worked for a company selling fume hoods and laminar flow benches just outside of RTP.