Dr. Duffy spent the beginning of her career training as a tissue engineer and applying advanced 3D imaging techniques to quantify engineered organs-on-a-chip before becoming an applications specialist for Zeiss. She attended Virginia Commonwealth University to earn her B.S. in Biomedical Engineering, and she pursued her graduate studies at Carnegie Mellon University in in Adam Feinberg’s lab where her research focused on engineering contractile skeletal and cardiac muscle microtissues. She continued her in vitro tissue engineering work through a collaborative post-doc in Boehringer Ingelheim’s SHINE program where she worked in Linda Griffith’s lab at MIT. For this project she focused on building 3D intestinal fibrosis models to measure Collagen Type I assembly from patient-derived cells in 3D microenvironments using laser scanning confocal and multi-photon imaging. From there she moved on to work as a scientist at United Therapeutics, working to build technology to 3D print human lungs for transplant patients. During this time, she established key cellular attachment, proliferation, and migration assays used to assess in-house developed bioinks, and she used a suite of ZEISS microscopes to assess cellularization of 3D printed scaffolds (CD7, LSM880, LSM980, Lightsheet 7). She is now the product specialist for the ZEISS Lattice Lightsheet 7 and is looking forward to helping scientists image live cells like they have never been able to before!
The drive towards live-cell imaging over long timeframes and at high volume speeds, while maintaining sub-cellular resolution, brings new challenges. Challenges which can be addressed by utilizing lattice light-sheet technology. Traditionally utilized live cell imaging techniques such as classical widefield, confocal and spinning disk methods expose the sample to light both at the focal plane of interest and above and below it. With light-sheet microscopy light exposure is minimized by illuminating only the focal plane of interest. Lattice light-sheets are long thin light-sheets which minimize phototoxicity, improve signal to noise and deliver subcellular resolution. With the gentleness of lattice light-sheet microscopy it is possible to capture dynamics at previously unreachable combinations of acquisition speed and resolution over hours and even days.
This talk will describe how the ZEISS Lattice Lighsheet 7 makes long-term volumetric imaging of living cells with subcellular resolution possible without having to change your standard sample preparation protocols to accommodate the instrument. With automatic alignment and easy acquisition workflows, lattice light-sheet imaging is now as accessible as using a standard inverted microscope.
Join us for this webinar to learn how ZEISS Lattice Lightsheet 7 allows you to discover the subcellular dynamics of life.