Transmission electron microscopy (TEM) is an imaging technique that provides atomic-level imaging and is typically reserved for TEM dedicated instruments. In this discussion, we will outline the contrast mechanisms in transmission imaging and highlight how STEM in FESEMs is becoming an increasingly adopted technique for a wide variety of applications ranging from imaging nanomaterials, thin sections of biological specimens, and diffraction studies and crystal orientation mapping at the nanoscale. The final part of this session will focus on 3D tomography enabled with a new STEM holder that allows the sample to be tilted and how digital image correlation techniques and 3D reconstruction can provide 3D volume imaging at the nanoscale.
Key Learning Objectives
- Learn about transmission imaging signal contrasts in a FESEM.
- Become familiar with tilt tomography in FESEM.