In this series "From Image to Results", explore various case studies explaining how to reach results from your demanding samples and acquired images in an efficient way. For each case study, we highlight different samples, imaging systems, and research questions. In this third episode, we showcase an imaging experiment performed on intestinal organoids treated with and without a Wnt-inhibiting drug, with the experimental goal to study the role of Wnt signaling in organoid formation.

Key Learnings:

• How to study the role of Wnt signaling in organoid formation
• How to use machine learning to segment outer organoid cell layers
• How to perform nucleus and cell body segmentation