ZEISS presents the first easy-to-use, highly productive workflow from a light to an electron microscope and vice versa.
Bridge the gap between structure and function by combining your scanning electron microscope with a light microscope: Overlay your fluorescence and ultraresolution images.
With Shuttle & Find you use a microscope to find and choose the structures or cells you are interested in. You image them and transfer the specimen with its specifically designed holder to your SEM. After the semi-automated calibration using fiducial markers you do not have to search for those regions again – Shuttle & Find handles this, saving you valuable time.
Correlate and overlay images obtained with your light microscope and SEM after image acquisition. This is especially useful for cell biology, neurobiology, studies of host-parasite interaction and analysis of symbiotic relations.
On a light microscope, first calibrate the sample holder, then define the sample region of interest that you want to examine under a scanning electron microscope. The sample positions are saved together with the image of the specimen.
After transferring the sample to the scanning electron microscope, reload the image with the regions of interest. Simply click the position marked in the image and the SEM moves to the corresponding location in a matter of seconds. Even the magnification transmitted with the image is taken into consideration.
If necessary, fine tune the system to align the light microscope image with that of the electron microscope. The correlation of specific fluorescence signals and ultra-structures considerably increases the results.
With up to 100x higher resolving power provided by the SEM, sub-cellular structures can be observed, identified and correlated with the functional information of the fluorescence signals with resolution down to a few nanometers.
Speed Up Your Correlative Workflow
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Increase the Productivity of Your Correlative Microscopy
pages: 2
file size: 2852 kB
A Reference Guide to Correlative Sample Preparation
pages: 48
file size: 671 kB
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file size: 1590 kB
High-Resolution Localization of Fluorescent Proteins Using Shuttle & Find for Superresolution and Scanning Electron Microscopy.
pages: 6
file size: 676 kB
Correlative Microscopy in Life Sciences
pages: 6
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Correlative Microscopy in Life Sciences
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Your Guide to the Widest Selection of Optical Sectioning, Electron Microscopy and X-ray Microscopy Techniques.
pages: 68
file size: 5952 kB
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