Atlas 5
Atlas 5
Large Area Imaging for SEM, FE-SEM & FIB-SEM and Sample-centric, Correlative Workflows for ZEISS Microscopes

Atlas 5

Master Your Multi-scale Challenge

Acquire EM Images Easier than Ever Before.

Correlate Images in Multiple Scales.

Atlas 5 makes your life easier: create comprehensive multi-scale, multi-modal images with a sample-centric correlative environment.

Atlas 5 is your powerful hardware and software package that extends the capacity of your ZEISS scanning electron microscopes (SEM) and ZEISS focused ion beam SEMs (FIB-SEM). Use its efficient navigation and correlation of images from any source, e.g. light- and X-ray microscopes. Take full advantage of high throughput and automated large area imaging. Unique workflows help you to gain a deeper understanding of your sample.

Its modular structure lets you tailor Atlas 5 to your everyday needs in materials or life sciences research.

Highlights

Easy-to-use, workflow-oriented GUI for automated imaging.

Easy-to-use, workflow-oriented GUI for automated imaging.

Acquire EM Images Easier and Faster than Ever Before

  • Acquire large sets of 2D or 3D nanoscale electron microscope (EM) images for hours or days, without operator supervision.
  • Collect single images over thousands of samples, or cover large areas with mosaics comprised of thousands of adjacent images.
  • Atlas 5 streamlines automatic image acquisition, using advanced preset and customizable protocols to produce consistent and reproducible results.
     
Light microscope and SEM images of an integrated circuit

Light microscope and SEM images of an integrated circuit, merged in the Atlas 5 correlative workspace.

Correlate Images from Multiple Sources

  • Bring together images from multiple sources. The correlative workspace of Atlas 5 makes it easy. Zoom in from the full macroscopic view of your sample down to nanoscale details.
  • Efficiently analyze and correlate images from multiple sources. Atlas 5 is your data hub for images from SEM, FIB-SEM, X-ray, light microscopes and any optical images, e.g. from your digital camera.
  • Build a seamless multi-modal, multi-scale picture of your sample with the sample centric workspace of Atlas 5.
3D visualization of plant tissue

3D visualization of plant tissue. Sample: courtesy of J. Sherrier, J. Caplan and S. Modla, University of Delaware, USA.

Understand Your Sample by Creating Unique Workflows

  • Understand your sample fully, in 2D and 3D with the graphical user interface of Atlas 5.
  • Design a workflow tailored precisely to the complexity of your experiment, no matter whether it’s a simple one-step task or a compound experiment.
  • A sophisticated workflow environment guides you from setup for automated acquisition to post processing and customized exports, and right on through to analysis.

Technology

Create Large Mosaic Images

Atlas 5 combines a 16 bit scan generator and dual super-sampling signal acquisition hardware with image processing and control software for your ZEISS electron microscope. Acquire images up to 32 k x 32 k pixels on SEMs and up to 40 k x 50 k on FIB-SEMs, with dwell times from 100 ns to > 100 s, adjustable in 100 ns increments. Save your images with eight or sixteen bits of intensity. 

Benefit from:

  • a reduced number of tiles to acquire and reduced computational complexity
  • reducing stage motion delay and areal fraction of each image “lost” to overlap
  • reduced number of overlap “seams”, leading to less beam damage and degradation of the sample

Acquire Images Easier than Ever Before

  • Define your exact region of interest and scan only the designated areas with predefined imaging protocols. Profit from reproducible results by developing protocols with ideal imaging conditions.
  • Keep images sharp during long acquisition times with the help of advanced auto-focus and auto-stigmation tools.
  • Image your sample whenever and wherever you need: Atlas 5 supports multiple sessions on multiple instruments.
  • Correlate images in Atlas 5’s sample-centric correlative workspace - bring together 2D images and 3D volume data from multiple instruments.
  • Import and align data from light, X-ray, electron and FIB-SEM microscopes to produce a single, consistent picture of your sample.

Correlate with X-ray Microscopy to Target Sub-Surface Sites in Your FIB-SEM

  • Correlate X-ray microscopy volume scans from your ZEISS X-ray microscope with surface features visible in your FIB-SEM.
  • Use the X-ray data to virtually localize sub-surface features in 3D to the precisely targeted FIB sites - even if they are not visible in situ.
  • Then navigate to those regions with confidence using your ZEISS FIB-SEM.

Modules

ZEISS Atlas 5 Comes with Multiple Offerings

Get to know ZEISS Atlas 5 modules, their features and their configurability. Extend the capacity of your ZEISS SEM and FIB-SEM with Atlas 5 as an option. Benefit from advanced capabilities of further modules and learn how they are combined.

Atlas 5 modules require Atlas 5 Base. Advanced Toolkit is necessary for optional Array Tomography, 3D Tomography and NPVE Advanced software modules. The Advanced Toolkit offline module includes Atlas 5 Base offline.

Atlas 5 Base

Workflow oriented high throughput acquisition platform. Fusion of images from multiple instruments, detectors and sessions in one correlative workspace. Import of one background and one overlay image (BMP, JPG, TIF, CZI, TXM). Protocol based automated 2D acquisition. Large framestore up to 32k x 32k, 100ns, 8bit/16bit, 2 channels. xROI imaging (exact regions of interest). Manual stitching of 2D mosaics.

Advanced Toolkit

Import of several background and overlay images, Advanced Image Correction (gradient corrections, radial correction, per channel corrections), Advanced Automatic Stitching, Export to Movie, Export to Browser-Based Viewer for web-enabled collaborative data exchange. Optimize protocols per acquisition. Export multi-channel data by channel or as blends. Integrated image review in a guided review workflow. Efficient review of acquired 2D SEM data and automated reacquisition of problematic images.

Array Tomography

Tools for array tomography setup: Clone Tool for section definition, Snap Section tool for automated section definition, Site Management functions for efficient sub-site definition across sections. Image stack viewer and image stack export options.

3D Tomography

3D FIB-SEM acquisition engine with automated sample preparation, exact region of interest (xROI) volume imaging, advanced sample tracking, adaptive slice thickness tracking, predictive drift correction and auto-tuning. Image stack viewer with FIB-SEM stack alignment, cropping and image stack export.

NPVE Advanced (Advanced Nanopatterning & Visualization Engine)

Advanced control over patterning geometry and parameters. Simultaneous beam control with full patterning and imaging support for ion and electron beams. Operation recipes for smart control of beam and GIS parameters for consistent milling and deposition control over the widest range of conditions. Set operations, 3D profiles and array builder tools to optimize design of experiments.

Analytics

Add EDS analytics to high resolution FIB-SEM tomography acquisition. Specify imaging and EDS conditions independently, including landing energy, dwell and spatial resolution in all three dimensions. Advanced acquisition engine automatically shifts between analysis and imaging conditions during acquisition. Flexible visualization allows simultaneous views of SEM images and processed elemental maps.

Atlas Image Viewer

Please click here for the Atlas Image Viewer

Downloads

ZEISS Atlas 5 Array Tomography

Image Your Serial Sections Fast and Efficiently – with Nanoresolution

Pages: 13
Filesize: 4,289 kB

ZEISS Atlas 5

Master Your Multi-scale Challenge

Pages: 20
Filesize: 6,350 kB

White Paper: ZEISS Atlas 5

Large Area Imaging with High Throughput

Pages: 6
Filesize: 4,056 kB

White Paper: ZEISS Atlas 5

Characterization of Solid Oxide Electrolysis Cells by Advanced FIB-SEM Tomography

Pages: 7
Filesize: 1,441 kB

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