AxioVision MAT Analysis Modules

AxioVision MAT for Image Analysis


On the trail of structures

Using this module you can determine the grain size of a wide variety of materials. Analyses can be performed on materials ranging from steel and nonferrous metals right through to ceramics. It is possible for analyses to be performed in accordance with DIN EN ISO 643, ASTM E 112 or ASTM E 1382. Additional standards are offered by the "Comparison Method".


Depending on your requirements and samples, you can choose from three measurement methods:

  • Automatic grain boundary reconstruction
  • Semi-automatic intercept method
  • Interactive comparison using comparative diagrams


Experienced users can define the measurement procedure in just a few steps. The report can be adapted to your requirements. Once it has been set up, even inexperienced users are then able to operate the system simply and conveniently.

You can manage the data in a clearly-structured archive. The test specifications are also saved here, in addition to the reports, images and data tables.

Analyzing grains

Automatically detected grain boundaries. To see a clipping of the user interface, click on the image.

The Automatic Method

This technique detects grain boundaries automatically and analyzes them. Artifacts are removed and grain boundaries reconstructed clearly, even in the case of complex structures. In addition to the average grain size, you can also determine the grain size distribution using this method.

The Chord Method

Using this semi-automatic method, you overlay a measurement grid onto the micrograph. You can choose from various sample grids for this purpose. The module marks the intercept points with the grain boundaries automatically. If necessary, you can add additional intercept points or remove them.

The Comparison Method

Using this interactive method, you can compare the micrograph with comparative diagrams. The Compare method offers the following comparative diagrams:

  • ASTM Plate I
  • ASTM Plate II
  • ASTM Plate III
  • ASTM Plate IV
  • SEP 151061
  • BS 4990


Analyze with flexibility

The Multiphase module enables you to analyze the phase distribution in your samples. In addition, the module can determine the size, shape or orientation of up to 32 phases, precisely and fully automatically. Using Multiphase it is also possible to measure parameters such as the gray value intensity or to define your own measurement parameters.

You can choose from over 300 measurement parameters that have been saved in AxioVision.

You decide which results you wish to document:

  • Percentage of the area represented by the selected phases.
  • Size of the individual particles of a phase.


You can classify the particles of a phase by area, diameter or circumference, for example. The class boundaries and number of classes can be defined as you wish.

In addition, for each phase the module also provides you with the number of particles and the percentage of the area they represent. The results are clearly displayed in a report.

You can save and manage all the data in an archive. Search and filter functions enable you to find your tables, images and reports there quickly and simply.


Layer Thickness Measurement

Precision in all layers

This module enables you to measure the thickness of your layers. Both single and multiple layers can be measured. The module analyzes simple layers just as reliably and conveniently as complex layers. These are detected automatically.


With just a few clicks of the mouse the layer thickness measurement delivers reproducible results. A structured role concept supports you in this: all the key settings for the measurement procedure are defined in administrator mode, while the actual measurements are then performed in user mode. Consequently, the layer thickness measurement is very simple to perform, even for inexperienced users.

When performing a measurement, you can define the measuring accuracy yourself. You can either specify the number of measurement chords or the distance between the measurement chords. Each layer is analyzed individually and precisely – irrespective of how many layers your sample has.

The measurement results are presented in the form of a clearly structured report. This contains:

  • measurement results
  • data on the sample
  • statistical analyses, such as the maximum and minimum chord lengths, and all other statistical values available in AxioVision

Comparative Diagrams

Assessing structural parameters

Comparative diagrams are a collection of mostly schematic micrographs. Certain characteristics have been assigned to each comparative diagram. These change gradually from image to image and may relate to the grain size, carbide precipitation in a steel or the quality of the preparation.


The AxioVision Comparative Diagrams module allow you to compare samples under the microscope and enables you to compare your micrographs with comparative diagrams on screen. This replaces the previous method involving the use of comparative diagram charts.

It is up to you how the images are displayed on the screen: you can decide whether you wish to compare the micrograph with one or several comparative diagrams.

In addition, the module also contains a wizard which you can use to generate your own comparative diagrams quickly and conveniently.

Comparative view with eight comparative diagrams. Click on the image to enlarge.
The Comparative Diagrams of following standards are delivered by default:

  • EN ISO 643
  • ASTM E 112
  • ASTM E 1382
  • BS 4990
  • SEP 151061
  • EN 10247
  • DIN 50602


The result is a table containing the evaluations relating to each image and a table containing statistical analyses. All data are saved in an archive. There, search and filter functions enable you to find the data you are looking for quickly and reliably.



Classify graphite particles

Analyze cast iron fully automatically and in accordance with the applicable standards with consistent quality. Shape and size of graphite particles will be analyzed according to EN ISO 945-1 and also obtain the spheroid number according to the current version of the standard (03/2009). The nodularity of vermicular graphite can be determined according to SAE J 1887.

The following functionality apply to all methods:

  • The module analyzes the results exactly according to the specifications of the standard.
  • The analyses are documented in test reports
  • The module saves all measurement data in a clearly structured archive. The data tables, images, reports and test specifications contained within the archive can be found quickly and conveniently using powerful search and filter functions

    Experienced users can define the measurement procedure in just a few steps. The report can also be adapted to your requirements. Once it has been set up, even inexperienced users are then able to operate the system conveniently and with little need for familiarization.


Analyze surfaces using digital elevation data

Generate height maps from your materials samples with this module. You can use either Z-stacks or interactively measured images, and display structures in three dimensions. All important information is contained in a topographic image. Height profiles can be printed out immediately as a measurement list.

You can use the Topography module to:

  • Generate height maps, either simply from Z-stacks or interactively by acquiring images in various focus planes
  • Display sharp areas in the form of a texture or height image
  • Flood height and projection images dynamically
  • Create an isometric 3D projection with texture, a grid or shadowing
  • Modify a projection or height image using look-up tables
  • Carry out surface roughness measurements in accordance with EN ISO 4287


Simplify routine tasks

If you need to create automatic measurement routines yourself: with the AutoMeasure module you can rapidly obtain precise results without complicated programming. With the help of the measurement wizard you carry out even complicated measurements within a few minutes.

Simply define the measurement parameters that you need and you can measure an unlimited number of images – while completely controlling the measuring process. Even automatic processes can be interrupted at any time and all parameters individually adjusted with the interactive function dialog.


AutoMeasure Plus

More than just measurement

Measure and document the entire structure of a microscopic sample automatically. This is now possible in a single measurement step with this AxioVision module. The result: fast, precise and reproducible quantitative analyses for your microscope application.

This AxioVision module includes the following functionality:

  • Segmentation
    This function offers threshold operators for monochrome and color images necessary to identify the objects based on gray color values. The objects can also be quickly identified at a mouse click using "region growing". These methods are supplemented by complex methods for segmentation, including dynamic and automatically generated threshold as well as edge detection.
  • Binary image processing
    Numerous functions ensure that the binary image is optimally prepared for measurement. They include linking, masking, and filling voids. Artifacts can be removed easily and contours smoothened. During "skeletonizing" objects are thinned to a 1-Pixel-line or separated from each other using a background skeleton.
  • Automatic measurement
    Morphometric measurement parameters are generated by the contour of the specimen. The software uses the binary image as a mask to calculate geometric and densitometric parameters from the original image. Their export into Excel enables statistical information about specimen details.

Interactive Measurement

The measure of things

Measure microscopic structures interactively. Simply select your desired parameter from more than 90 different options. Our measurement wizard then records all the data in the sequence that you have specified. The results may be archived together with the image or exported as a table.

Online Measurement

Interactive and live

With the Online Measurement module you can measure materials structures in your online images interactively. There is no need to acquire any images. You measure your samples directly on the live window of the camera. Visual inspections can be carried out quickly and conveniently on screen.

All the measurement tools that you use for your acquired images can also be used here. You select the desired parameters from a choice of up to 90 options. Eyepiece reticles are therefore no longer required. Your results can be archived together with the image or exported into a clearly presented table.


Ask your ZEISS contact about AxioVision Image Analysis now!