ZEISS Point Counting Workflow

From the Solutions Lab for light microscopy

Determining the estimated volume fraction of a certain constituent or phase of interest in a sample can give you further insight into its properties. Point counting is a widely used and standard workflow for doing just that and predicts 3D information from a 2D cross-section.

To streamline this process, a workflow was created that combined several layers of customization and microscope control into a solution that is easy to use and ensures results that accommodate industry standards.

Determining the estimated volume fraction of a certain constituent or phase of interest in a sample can give you further insight into its properties. Point counting is a widely used and standard workflow for doing just that and predicts 3D information from a 2D cross-section.

To streamline this process, a workflow was created that combined several layers of customization and microscope control into a solution that is easy to use and ensures results that accommodate industry standards.

Highlights

Area of Research:

  • Natural Resources

Sample Types:

  • Any, Metals

Related Solutions:

  • Grain Size Colormap workflow
Point counting workflow
Point counting workflow.

Workflow

The module workflow consists of a number of steps:

  1. Image acquisition
  2. Grid overlay customization
  3. Manual classification of how many points on grid overlay constituent of interest
  4. Move to a new field of view and perform analysis again
  5. Continue until desired relative accuracy is met
  6. Complete reporting of individual measurements and overview statistics

Required components

  • ZEISS Light Microscopes
  • ZEN
  • ZEN Image Analysis
  • ZEISS Point Counting Workflow

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Problems ​we can help you solve:

  • automation of your imaging process or workflow
  • advanced image analysis and corrections
  • automation of your ROI (region of interest) search
  • tailoring flexible and adaptable workflows for your configuration
  • even, small applications that turn 20 clicks into one

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