ZEN Image Acquisition Modules

Image Acquisition

Image Acquisition

ZEISS ZEN for Image Acquisition

Multi-Channel Image Acquisition Module

Multi Channel

With ZEN Multi Channel image acquisition module you create images with multiple channels. To do this, with widefield systems you combine any number of fluorescence channels with images in transmitted light, e.g. phase contrast, according to your requirements. A special feature here is that you can select your dyes from the integrated database. This already contains data sets for more than 500 fluorochromes and can be expanded as required.

For each fluorescence excitation wavelength, the relevant channel is acquired with the optimal exposure time. An independent exposure time and microscope setting can also be assigned to each channel. The advantage: you structure your experiments flexibly and perform even highly complex multichannel experiments simply.

Z-Stack Image Acquisition Module

Z Stack

ZEN  Z Stack module helps you to acquire axial image stacks on your ZEISS microscope with motorized focus drive. The image acquisition program controls the Z-drive of your stand in precisely the right increments and synchronizes it with acquisition. ZEN can calculate the increments fully automatically, if required, or you can allocate them manually.

A special feature here is that from now on you create your Z Stacks with graphical support. This assistance is even available to you dynamically during acquisition. The size of your experiment, which is saved in a compact .CZI file, is limited only by the travel range of your system and the minimum increment.

Extended Focus Image Acquisition

Extended Focus

With the Extended Focus module you generate images with extended depth of field from previously acquired Z-stacks. The ZEN module extracts sharply imaged details from existing images at different focus positions and combines them to create a new image. The advantage: you always have maximum depth of field in all regions of your images in transmitted light, reflected light and fluorescence contrast.

Tiles and Positions

Tiles & Positions

With the optional ZEN Tiles & Positions module and a motorized stage you scan your specimen automatically, making it possible to analyze large areas. A large, virtual overview image can be generated in this way from numerous individual images. This allows you to navigate over the specimen like a map and zoom easily into any regions of interest. Tiles & Positions and ZEN align the individual images precisely in relation to one another, providing the optimal basis for further analyses.

A special feature here is that a software wizard helps you to mark positions on multiwell plates, multi-chamber slides, slides and dishes interactively and locate them with a click of your mouse.

Experiment Designer

Experiment Designer

Would you like to plan complex experiments in advance, but retain complete control at all times? From now on ZEN will help you do this! Using the Experiment Designer module you will even be able to create images in several combined image dimensions with just a few clicks of your mouse, and after only a short training period. You plan combinations or repeats of your image acquisition tasks. such as time series, Z-stacks, tile/multiposition images and channels, in a clearly visualizable form using experiment blocks.

Repeated steps can be achieved very easily using loops, and pauses or interactions can be defined if your experiment extends over a long period and requires interactive intervention. The Experiment Designer supports an almost unlimited number of blocks in your experiments, which can naturally be saved and reloaded at any time. You plan, ZEN controls.

Time Lapse Image Acquisition Module

Time Lapse

With the ZEN Time Lapse module for widefield systems you control your microscope, camera and imaging software from ZEISS. This allows you to document dynamic processes in your specimens with a compact image sequence. ZEN controls the exposure setting for your microscope here with millisecond precision. With the Time Lapse module, damage is no longer caused to specimens as a result of unnecessarily long exposure times – an important requirement for your quantitative analyses of living specimens.

Your most impressive images can be exported at any time into common video formats, such as AVI or MOV, for demonstrations and publications. The advantage: images are acquired with maximum speed and are only limited in size by the free space available on your hard drive.

Manual Extended Focus

Manual Extended Focus

With the Manual Extended Focus module you use ZEN to create images without being restricted by your microscope's depth of field. The software module extracts the sharp details at different focus positions from the interactively acquired Z-stacks and combines them into an image with maximum depth of field. Using the live image display, additional images can be added to the intermediate result.

The advantage: you always have maximum depth of field in all regions of your images. This even applies irrespective of the contrast technique used – whether transmitted light, reflected light or fluorescence, thanks to our wavelet algorithm.

Autofocus Module


With the Autofocus module for widefield systems you determine the optimal focus position for your specimen in transmitted light, reflected light and reflected light fluorescence. ZEN “learns” the ideal position during your everyday work on the light microscope and is therefore trained continuously with regard to correct focusing. No calibration procedure is required for this and the focus is recorded automatically for all objectives and contrast techniques.

The additional advantage for you: in the case of images that are acquired over time or at different positions, the system continually refocuses automatically.

Panorama Module


This ZEN module is ideal if your specimens no longer fit into the image field. With Panorama you create high-resolution overview images from individual images. The software puts the images together with pixel precision. The advantage: even images that have been acquired offset from each other can be joined precisely using ZEN and Panorama. This means that important details from the individual images of your microscope specimen are recorded in a single image.

A special feature here is that you can use the live image as a ‘joystick’ and therefore acquire the individual images even more quickly.