ZEISS ZEN Image Processing
ZEISS ZEN
Image Processing
Image Processing

ZEISS ZEN for Image Processing

Colocalisation

The Colocalisation module can be used to unmix the overlapping signals of different fluorescence channels.
To do this, the ZEN software makes use of modern methods, such as the automated threshold value method of Costes et al. Meaningful results on the colocalization of proteins can be obtained as a scatter plot, image and data table

If you only want to analyze certain areas of the image, you can simply mark the corresponding region directly in the image and scatter plot. Spectral Colocalisation for ZEN then supplies the data both for the whole image and the selected regions.
Of course you can also export your measured values in CSV format and process them further using a spreadsheet.

Deconvolution

Due to the optical properties of the microscope system, light from the areas above and below the focal plane also enters the image. This means that fine structures on the specimen can no longer be picked out.

It is precisely here that the 3D Deconvolution software module starts, by calculating stray light in the image stack back to its place of origin. The distorted specimen structure unfolds and is displayed more sharply. This technique also reduces any image noise that may be present, making a decisive contribution to the first-class image quality of your image stacks and 3D models.

3D VisArt

With 3D VisArt you can display multi-dimensional image stacks as 3D volume models. The ZEN module processes up to 8 different channels and, besides the spatial image coordinates, also takes the time lapse information relating to your images into consideration.

You can position a calculated three-dimensional model interactively according to the view that you require and provide it with annotations, coordinates and scale axes. It goes without saying that 3D VisArt also provides you with powerful tools for the interactive measurement of distances and angles. Would you like to present or publish the result afterwards? If so, simply export the images and animations into common image formats, such as TIF, JPG or AVI.