MERLIN Scanning Electron Microscope
MERLIN

MERLIN for Life Sciences

Complete image analysis and characterization of biological samples.

Highest Beam Current in a Nanometer Size Spot

Based on the new GEMINI II column and the ZEISS Complete Detection System, MERLIN is the ideal solution for complete image analysis and characterization of biological samples. With the highest beam current in a nanometer size spot, MERLIN provides the fastest imaging of large fields of view at FE-SEM resolution, perfect for streamlined imaging of large samples such as cells or tissue sections of brain, kidney, pathology, plant and forensics. 

Image collection speed is further enhanced by sample transfer rates of less than 60 seconds. A simultaneous view of up to four detector signals allows for image comparison without alignment changes for all non-conductive, biological materials due to MERLIN’s unique charge compensation with in-situ cleaning.  Novice users are able to achieve professional results using the automated column alignment of MERLIN which quickly attains optimum imaging conditions.

In combination with the ATLAS software, high resolution, extreme field of view imaging is possible with MERLIN, with image sizes of up to 32k by 32k pixels, perfect for examining large sections from brain, kidney, nanopathology and forensic samples or other tissues.  MERLIN, in combination with the 3View ultramicrotome, can also be used for high-resolution, 3D-imaging of tissues and even whole organisms, such as Drosophila, zebrafish or plants.

 

What Our Customers Say

Dr. Matthias Schmidt

Specialist for Electron- and Helium-Ion Microscopy

"The Merlin as well as the ORION form the high-resolution imaging part of the correlative workflows for chemical imaging of microbiological samples at the ProVIS centre.

The instruments are complentary as the Merlin allows for STEM and analytics (EDX) whereas the ORION is perfect for imaging of cell surfaces and high-precision nano-lithography. From electron/ion-optics to software every part of the microscopes is well designed. It is a pleasure to work with these instruments!"
 

Dr. Matthias Schmidt
Helmholtz Centre for Environmental Research – UFZ
Specialist for Electron- and Helium-Ion Microscopy
June 30, 2015

Highlights

New GEMINI II column and the ZEISS Complete Detection System

  • Complete image analysis and characterization of biological samples
  • Fastest imaging of large fields of view at FE-SEM resolution
  • Streamline imaging of large samples such as cells or tissue sections of brain, kidney, pathology, plant and forensics 

 

 

Image collection speed with transfer rates of less than 60 seconds

  • Image comparison without alignment changes for all non-conductive, biological materials
  • Quickly attain optimum imaging conditions

 

ATLAS Large Area Imaging

  • Acquire images up to 32k x 32k pixels with dwell times from 100 nanoseconds to > 100 seconds
  • Create large image montages, automatically moving from image tile to tile, resulting in extreme field of view
  • Reduces the number of tiles resulting in less beam damage and increased high throughput performance
  • Examine large sections from brain, kidney, nanopathology and forensic samples or other tissues

Gemini II

GEMINI ll Electron Optics

Complete Detection System

The primary electron beam generates secondary electrons and backscatter electrons. Secondary electrons precipitate directly from the topmost nanometers of your sample with energy of less than 50 eV and show the topography of the surface. The electric field of the GEMINI column attracts these electrons and the excitation lens directs them to the annular InLens Detector. Depending on the surface condition of your sample, MERLIN® detects the secondary electrons over a wide angular range.
 
Backscatter electrons are generated below the surface. Their energy level is similar to that of the primary electrons hitting the sample. Their number is greater than that of the secondary electrons and they provide highly specific information about the material characteristics of your sample. They are attracted by the electric field of the GEMINI column and projected into the column. The difference in energy ensures that secondary electrons and backscatter electrons are displayed on different trajectories by the Beam Booster. The backscatter electrons are detected by the Energy Selective Backscatter Detector (EsB) and selected according to their energy level.
 
If the angle is too small, the backscatter electrons do not make their way into the column, but are detected by the AsBR-Detector at the pole of the lens. The small-angle backscatter electrons provide highly specific information about the crystalline structures of your sample. The angle-dependent detection of backscatter electrons is called Angle selective Backscatter Electron Detection or AsB-Detection.
The AsB-Detector of MERLIN® is completely integrated into the GEMINI lens and detects backscatter electrons with minimal working distance. The AsB-Detector no longer needs to be aligned to the optical axis.

3View

Fast and Convenient 3D Imaging for Tissue Samples in the FE-SEM

Combine your MERLIN with 3View technology from Gatan Inc. to acquire high resolution 3D data from resin embedded cell and tissue samples. In the shortest possible time.

3View is an ultramicrotome inside the SEM chamber. The sample is continuously cut and imaged. You produce thousands of serial images in a single day – each perfectly aligned because they are all generated from one fixed block.

Your complete solution from Carl Zeiss

Profit from MERLIN 3View for best low voltage performance and highest flexibility. Your system runs with unbeatable long term stability without any user intervention.

 

Largest field of view

ZEISS GEMINI technology delivers up to 32k x 24k pixels in one scan at nm resolution. You image even large fields of view without blurring at the edges. And you won’t need to stitch images for most applications.

 

Highest speed

Depending on your application you get your 3D results up to 10 times faster with 3View. Achieve highest scan speeds with MERLIN without compromises in resolution.

Watch the Webinar

Certified Refurbished Product

Your affordable pre-owned, re-worked ZEISS electron microscope

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Downloads

ZEISS Merlin

From Imaging to Your Complete Lab: Analytical Power for the Sub-Nanometer World

Pages: 14
Filesize: 3,201 kB

White Paper: Large Volume Imaging of Eye Muscle by SIGMA VP and 3View

Serial Block Face Imaging

Pages: 8
Filesize: 1,984 kB

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