The super-resolution microscope Elyra 7 takes you far beyond the diffraction limit of conventional microscopy: With Lattice SIM² you can now double the conventional SIM resolution and discriminate the finest sub-organelle structures, even those no more than 60 nm apart. You don‘t need to sacrifice resolution when imaging at high speed using only the minimal exposure needed for life observation. Elyra 7 enables you to combine super-resolution and high-dynamic imaging – without the need for special sample preparation or expert knowledge of complex microscopy techniques.
- Resolve structures down to 60 nm.
- Observe live cell dynamics at up to 255 fps.
- Accelerate image acquisition in all three dimensions.
- Get the sharpest sectioning in wide-field microscopy.
- Utilize a wealth of imaging techniques on one platform.
Learn how to reveal sub-organelle structures down to 60 nm, capture highly dynamic processes at exceptional resolution, get outstanding out-of-focus light suppression, and make super-resolution acquisition faster than ever before.
With SIM², a novel image reconstruction algorithm raising the SIM technology to a new level, you can now double the conventional SIM resolution. Lattice SIM² comes with outstanding out-of-focus light suppression, giving you the sharpest sectioning in widefield microscopy even for highly scattering samples. SIM² image reconstruction robustly reconstructs all structured-illumination-based acquisition data of your Elyra 7 – with minimal artefacts – for living and fixed samples.
While doubling the classic SIM resolution, SIM² gives you gentle imaging of living and fixed specimens at high speeds of up to 255 fps. Combine SIM² with Burst and Leap modes to make super-resolution acquisition faster than ever before. With SIM Apotome mode, even lossless acquisition can be achieved, meaning for every reconstructed image just one raw image is needed! Or make use of Elyra 7 Duolink to image two differently stained structures simultaneously and use the multiple colors to boost resolution even further.
Time lapse imaging of the endoplasmic reticulum (Calreticulin-tdTomato) in a Cos-7 cell reveals highly dynamic structural changes.
Elyra 7 handles virtually all types of samples, including photo-sensitive cell cultures, scattering C. elegans and plants or tissue sections of up to 100 µm thickness. Elyra 7 includes several microscopy techniques: Lattice SIM², SIM² Apotome, widefield DIC, SMLM and TIRF. You can correlate images of the same sample acquired using any or all of all these techniques to multiply the insights from your specimen. You can even combine Elyra 7 with a variety of other imaging systems such as LSM with Airyscan or scanning electron microscopy in a correlative workflow.
In Lattice SIM, the sample area is illuminated with a lattice spot pattern instead of grid lines as in conventional SIM. This leads to a dramatic increase in imaging speed. In addition, the lattice pattern provides higher contrast to allow a more robust image reconstruction. Since the sampling efficiency of lattice pattern illumination is 2× higher compared to classic SIM, you need less laser dosage for sample illumination. This lattice illumination makes SIM a preferred live cell imaging technique. The strongly improved photon efficiency of lattice illumination allows you to increase the imaging speed while achieving higher contrast and lower photo dosage.
SIM² is the novel, groundbreaking image reconstruction algorithm that increases the resolution and sectioning quality of structured illumination microscopy data. SIM² is compatible with all SIM imaging modes of your Elyra 7 and fully integrated in the ZEN software.
Unlike conventional reconstruction algorithms, SIM² is a two-step image reconstruction algorithm. First, order combination, denoising and frequency suppression filtering are performed. All the effects resulting from these digital image manipulations are translated into a digital SIM point spread function (PSF). The subsequent iterative deconvolution uses this very PSF. Similar to advantages of using experimental PSF for deconvolution of hardware-based microscopy data, the SIM² algorithm is superior to conventional one-step image reconstruction methods in terms of resolution, sectioning and robustness.
Lattice SIM² enables you to perform multi-color imaging at resolution down to 60 nm for conventionally stained samples. Due to its small size, three-color imaging of the synaptonemal complex has previously been possible only using complex methods like super-resolution imaging of three-fold expanded samples. Lattice SIM² resolves the two strands of SYCP3 (lateral elements) as well as SYCP1-C (C-terminus of transverse filaments) without special sample treatment or staining for distances well below 100 nm. More importantly, the three-color image provides structural information about the distances between the proteins SYCP3 and SYCP1. Even within the SYCP1 protein, the different labeled N- and C-Terminus can be clearly separated with less than 50 nm resolution between the two labels.
I remember seeing the first results. I could only laugh as I was simply amazed. My next reaction was to email some of the key users who could immediately benefit. From the tissue neurobiologists, to cell and molecular immunologists, to those working with yeast and those working with bacteria, all of them are already gaining from SIM².Peter O‘Toole, Head of Imaging and Cytometry, University of York
Live cell imaging with a widefield system often suffers from out-of-focus blur or background signal. These effects can decrease contrast and resolution of your images. The SIM Apotome acquisition mode of Elyra 7 uses structured illumination to give you fast optical sectioning with crisp contrast and high lateral and axial resolution.
The SIM Apotome acquisition mode in combination with the SIM² reconstruction algorithm now allows you to further tune the gentleness of your fast live-cell imaging with high contrast and resolution. Or use your new optical sectioning speed to increase your productivity when acquiring large sample areas or large volumes at different magnifications.
SIM² Apotome: Comparison of widefield and SIM² Apotome single plane images of Cos-7 cells stained for microtubules (anti-alpha-tubulin Alexa Fluor 488, green) and nuclei (Hoechst, blue). Objective: LD LCI Plan-Apochromat 25× / 0.8 Imm Corr
In single-molecule localization microscopy (SMLM), fluorescent molecules are sparsely activated so that only one out of many will be in its on-state within a single point spread function (PSF). This lets you determine its center of mass with a localization precision that far exceeds the extension of the PSF. Once recorded, the molecule is turned to its off-state and the cycle of activation/deactivation is repeated until all molecules are captured. The localizations are plotted in a new image to create the super-resolution image. With Elyra 7 you can use SMLM techniques such as PALM, dSTORM and PAINT to achieve lateral resolution of 20 – 30 nm. The ZEN software will seamlessly perform the image reconstruction of your data.
In addition, Elyra 7 provides you with 3D SMLM mode based on PRILM technology. The PSF is reshaped for encoding the Z position so while acquiring only one plane, you get volume information of 1.4 µm depth at 50 – 80 nm axial resolution. Thus, you can acquire 3D data from a whole cell with consistent molecular precision.
Investigation of living samples very often focuses on interactions of different proteins or organelles. Simultaneous imaging of the involved structures is key to proper understanding of these highly dynamic processes. Equip your Elyra 7 with a Duolink adapter to operate two sCMOS cameras and use all the advantages that a widefield-based technology can offer:
- Perform true simultaneous two-color imaging within your entire field of view – without any delays within the image such as can occur when using scanning-based technologies or during consecutive data acquisition of different channels.
- Acquire a super-resolved real-time snapshot of an entire living cell by picking a low exposure time.
- Increase the productivity of your fixed cell experiments by doubling the amount of information gained during the same amount of time.
- Image any possible color combination with the two cameras, and with minimal signal crosstalk as enabled by the integrated multi-bandpass emission filters.
- Acquire 4-color images without the need for mechanical filter change – making your multi-color experiments even faster.
- Perform multi-color SMLM experiments on the two sCMOS cameras.
Cos-7 cell expressing the endoplasmic reticulum marker Calreticulin-tdTomato (magenta) and mitochondrial marker Tomm20-mEmerald (green) was simultaneously imaged for two colors. The movie shows high dynamic interactions of the ER and mitochondria.
The diffusive and especially the ballistic movement of small vesicles in cells can be captured only when super-resolution and high-dynamic imaging are possible at the same time. With the Burst processing of 2D time lapse data, Elyra 7 is able to generate super-resolution images at 255 Hz in a large field of view and even acquire two colors simultaneously in both Lattice SIM and SIM Apotome acquisition modes.
U2OS cell expressing Rab5-mEmerald (green) and tdTomato tagged Golgi associated transport marker (magenta). Simultaneous dual-color acquisition with an exposure time of 1.5ms / phase for a FOV of 1024 × 1024 pixel (64 µm × 64 µm).
Elyra 7 Leap mode accelerates the volume imaging speed three times and at the same time decreases the light dosage on your sample. While still capturing all the finest details, the entire volume (18 planes) of the U2OS cell expressing Calreticulin-tdTomato was imaged at 38 volumes / min speed in Lattice SIM acquisition mode. For SIM Apotome acquisition mode, you can expect up to three times higher volume imaging speed.
U2OS cell expressing calreticulin-tdTomato to visualize the endoplasmic reticulum. The time series shows a maximum intensity projection of the volume data set.
Studying the Components of the Cytoskeleton
Due to the fine structures of cytoskeleton components, for example the actin network or microtubular filaments, imaging far below 100 nm is often performed with super-resolution techniques. Lattice SIM² allows you to gain much more structural information from your samples compared to conventional SIM techniques. It not only operates with a resolution of down to 60 nm but also provides markedly improved sectioning quality.
Understanding Biological Processes
Due to its unique lattice structural illumination, Elyra 7 combines high speed imaging with incredible light efficiency, low photon dosage and sensitivity. You can observe cellular, subcellular, and even sub-organelle structures in living specimens in 2D and 3D over time. Whether you are interested in the dynamics of mitochondrial movement, fusion and fission or budding of the endoplasmic reticulum, Elyra 7 Lattice SIM² provides you with the necessary live cell compatibility at super-resolution.
U2OS cell expressing Tomm20-mEmerald. Objective: Plan-Apochromat 63× / 1.4 Oil
Simultaneous imaging of the endoplasmic reticulum (Calreticulin-tdTomato, magenta) and microtubules (EMTB-3xGFP, green) in a Cos-7 cell reveals highly dynamic interaction of these organelles. Objective: Plan-Apochromat 63× / 1.4 Oil
Excellent Sectioning at Incredible Speed
SIM² Apotome is your flexible live cell imaging method for experiments that do not require the highest spatial resolution but rely instead on excellent sectioning quality. SIM² Apotome is superior to conventional confocal microscopy in terms of lateral and axial resolution as well as volume acquisition speed while it is also very gentle to your sample. The high NA (1.4) 40× magnification images almost reach the resolution and sectioning capabilities of a conventional SIM microscope, while multiplying acquisition speed.
SIM² Apotome time lapse data of LLC PK1 cells expressing H2B-mCherry (magenta) and α-Tubulin mEmerald-GFP (green). Data shown as maximum intensity projection of 12 planes over 3.7 µm depth. Objective: LD LCI Plan-Apochromat 25× / 0.8 Imm Corr
SIM² Apotome time lapse data of Cos-7 cells expressing the endoplasmic reticulum marker Calreticulin-tdTomato. Data shown as maximum intensity projection of 12 planes over 1.4 µm depth. Objective: Plan-Apochromat 40× / 1.4 Oil
Fast Tile Scanning of Very Large Areas
The high-speed performance of SIM Apotome acquisition mode allows for fast tile scan imaging of very large areas at excellent sectioning quality. A mulberry section of 11.1 mm² × 11 µm size was imaged using Nyquist sampling in all three directions and in two colors in less than 2 minutes. Similar speeds also were achieved for a cross section of a leaf.
SIM² Apotome volume tile scan image of a thin mulberry section with an EC Plan-Neofluar 10× / 0.3 air objective. Data shown as maximum intensity projection over 11 µm depth. Sample: “Maulbeere” from TS-Optics Set Dauerpräparate Botanik 25St.
SIM² Apotome volume tile scan image of a leaf cross section, imaged with an EC Plan-Neofluar 10× / 0.3 objective. The image shows a maximum intensity projection of a Z stack. Sample: “Leaf” from TS-Optics Set Dauerpräparate Botanik 25St.
Defining Specific Needs for Speed and Resolution
The need for higher imaging speeds and decreased light dosage is almost unlimited. The robustness of Elyra 7 structured illumination patterns plus the image reconstruction software allow a significant reduction to the number of phase images required for both Lattice SIM and SIM Apotome acquisition modes, while decreasing the resolution only slightly. Lattice SIM acquisition can be operated at 9 phase images per frame while 3 phase images per frame are sufficient for SIM Apotome, increasing the imaging speed by 44 % and 66 %, respectively.
Cos-7 cell expressing EMTB-3xGFP (green) and EB3-tdTomato (magenta) shows dynamic movement of microtubules. Imaged in Lattice SIM 9 phase mode.
Actin dynamics in a Cos-7 cell expressing LifeAct-tdTomato were imaged with the SIM Apotome 3D Leap mode over time.
Resolving Details Hiding in the Depth
Despite being a structured illumination-based microscope, Elyra 7 Lattice SIM² as well as SIM² Apotome also provide you with super-resolution and high-quality sectioning in thick or scattering samples. The combination of robust illumination patterns and excellent image reconstruction technology enabled us to image throughout an entire murine brain section of ~80 µm thickness expressing the neuronal marker Thy1-eGFP.
Discovering the Diversity of Life
You can investigate living or fixed, small or large, thin or thick specimens using Elyra 7 Lattice SIM², SIM² Apotome or SMLM modes. Whether you study vesicle dynamics in cells or yeast or you want to unravel the architecture of plants, C. elegans, zebrafish, D. melanogaster or bacteria, with Elyra 7 you will experience easily accessible super-resolution imaging for your favorite model organism and many other specimens.
Lattice SIM² 3D image of a C. elegans larvae. Image shows a maximum intensity projection. Sample courtesy of Mango Lab (University of Basel, Switzerland)
Lattice SIM² time lapse images of living yeast expressing GFP-coupled membrane marker and mCherry-coupled Golgi associated protein. Sample courtesy of C. MacDonald, G. Calder & P. O’Toole (Department of Biology & Bioscience Technology Facility, University of York, UK)
SIM² Apotome 3D image of a leaf of a living A. thaliana sample shows the microtubules (Tubulin-GFP) in the upper three cell layers. Sample and data curtesy of G. Calder and P. O’Toole (Department of Biology & Bioscience Technology Facility, University of York, UK)
SIM² Apotome: Zebrafish embryo expressing a vascular marker fli1-EGFP was imaged in 3D. Maximum intensity projection of the tile scan Z stack data set. Sample courtesy of Haass Lab (MCN, University of Munich, Germany)
A Journey Through Different Scales
Biological samples often contain completely different types of information at different length scales. Being able to collect low to high resolution data in the same sample not only makes you more productive but also interconnects the findings and puts them in context to give you the whole picture.
SIM² Apotome and Lattice SIM² images of a murine brain expressing the neuronal marker Thy1-eGFP. The images show the color-coded or maximum intensity projections of the volume data.
Single Molecule Localization Microscopy (SMLM)
SMLM encompasses techniques such as PALM, dSTORM, and PAINT. With high power lasers across the visible spectrum and dual camera detection, Elyra 7 allows researchers to gain access to a broad range of dyes, markers and fluorophores in almost any possible combination.
SMLM allows you to map precise locations of individual proteins.
Detect two channels with molecular precision.
Untangle molecular relationships in z with confidence.
ZEISS Elyra 7 with Lattice SIM²
Your Live Imaging System with Unprecedented Resolution
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Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy
file size: 6578 kB
Introducing Lattice SIM for ZEISS Elyra 7
Structured Illumination Microscopy with a 3D Lattice for Live Cell Imaging
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