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Semen Analysis

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Semen analysis is a very important step before any artificial insemination is carried out. Semen is a fluid containing sperm cells, which is released during ejaculation. A normal sperm count is above 20 million sperm per milliliter of semen. The volume of semen is normally more than 2 milliliters. Beside the pure volume of sperm cells a semen analysis (seminogram or spermiogram) includes the microscopic evaluation of

  • sperm concentration: sperm number is counted (Mio cells/ml)
  • sperm motility/vitality : measures, how many sperm are moving progressively, non-progressively, or are immobile.
  • sperm morphology: evaluation of normal versus abnormal shape.

To create the seminogram you observe living sperm cells in phase contrast and stained sperm cell smears in transmitted light brightfield or fluorescence. Only vital sperms and healthy oocytes lay the foundations for a successful fertilization.

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Immobile Sperm Eosine Y Vitality Test
Immobile Sperm Eosine Y Vitality Test
Living Spermatozoa in Positive and Negative Phase Contrast

Recommended Products for Semen Analysis

ZEISS Axiolab 5
Axiolab 5 combines excellent optics and the options of phase positive and phase negative contrast. So, you can use this upright light microscope for manual semen analysis and visualize even smallest sperm cells with highest contrast. Furthermore, Axiolab 5 builds the basis for computer assisted sperm analysis systems, such as CEROS from Hamilton Thorne.

ZEISS Axiolab 5 for semen analysis

ZEISS Axioscope 5
Axioscope 5 offers flexibility and comfort to fit your advanced semen analysis needs. By combining brightfield, phase contrast and fluorescence you can different morphology and vitality checks with one system. The ergonomic design provides fatigue-proof working with the microscope. The dedicated fluorescence LED light source Colibri 3 is maintenance-free and keeps the overall footprint of the system compact. Axioscope 5 is your reliable semen analysis system, when it goes beyond routine brightfield and phase contrast observation.

ZEISS Axioscope 5 for semen analysis