Interactive Tutorials - Spinning Disk Fundamentals

Interactive Tutorials

Basic Microscopy

Objective Specifications

Microscope objectives are precision optical systems that feature a wide range of magnifications, numerical aperture, immersion media, specialized contrast applications, and other properties. Information pertaining to the specifications of microscope objectives are inscribed on the decorative barrel by the manufacturer. This interactive tutorial examines the specification found on typical objectives.

There is a wealth of information inscribed on the barrel of each objective, which can be broken down into several categories. These include the linear magnification, numerical aperture value, optical corrections, microscope body tube length, the type of medium the objective is designed for, and other critical factors in deciding if the objective will perform as needed. The figure below outlines the specifications that are included in this interactive tutorial.

Contributing Authors

Rudi Rottenfusser - Zeiss Microscopy Consultant, 46 Landfall, Falmouth, Massachusetts, 02540.

Tony B. Gines and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.