ZEISS Campus

Interactive Tutorials

Spectral Imaging Tutorials

Additive Properties of Emission Spectra

This interactive tutorial explores how multiple spectra can be added to produce a composite emission spectrum similar to those encountered in spectral imaging of specimens labeled with multiple fluorophores.


Spectral Imaging with Linear Unmixing

Explore how mixed fluorophores having highly overlapping emission spectra can be separated into individual components using spectral imaging and linear unmixing techniques. This tutorial contains several examples with fluorophores emitting in the green and red spectral regions.


Emission Fingerprinting with Lambda Stacks

Use this tutorial to examine how lambda stacks can be used to extract information about individual spectral profiles in specimens labeled with highly overlapping fluorophores.


LSM 700 Light Pathways

The LSM 700 laser scanning confocal microscope from Carl Zeiss is designed for efficient separation of signals by efficient splitting of the emission using the variable secondary dichroic (VSD) beamsplitter to prevent crosstalk and enable spectral imaging as well as linear unmixing of highly overlapping fluorophores.


Spectral Imaging FRET with Biosensors

Spectral imaging of FRET biosensors using fluorescent proteins is an emerging technique for the analysis of events in cell biology. This tutorial explores the performance of a cameleon calcium biosensor and a caspase apoptosis indicator in spectral imaging.


Fluorescent Protein FRET with Biosensors

Spectral imaging has been very useful for the examination of fluorescent protein biosensors to determine the presence or absence of FRET in response to a biological stimulus.


3-Channel QUASAR Detection Unit

The ZEISS QUASAR photomultiplier detection technology is based on a filter-free system that guides the desired wavelength range to the target detector using adjustable optical wedges and slider light stops.


34-Channel QUASAR Detection Unit

Employing a special 32-channel photomultiplier, the ZEISS multichannel QUASAR detection unit is ideal for enhancing lambda stack acquisition speed for live-cell imaging experiments.