
Explore ZEISS LSM with Lightfield 4D
With the launch of the new generation of ZEISS confocal laser scanning microscopes, we are proud to provide you with our latest innovation in high-speed imaging of living organisms: ZEISS Lightfield 4D.
Lightfield 4D expands your confocal with the ability to capture complete volumes – instantly, with a single snap. Capture the fastest movements within whole organisms at up to 80 volumes per second.
Start your experiments immediately without the need to adjust sample preparation.
Capture up to
Built for New Discoveries
Key features of ZEISS LSM Lightfield 4D at a glanceImage 1: Zebrafish heart. Courtesy of Stone Elworthy and Emily Noël, School of Biosciences, University of Sheffield, UK. Data acquired at Wolfson Light Microscopy Facility in the School of Biosciences at the University of Sheffield.
Image 2: Developing zebrafish ear. Courtesy of Tanya Whitfield, Sarah Baxendale, School of Biosciences, University of Sheffield, UK. Data acquired at the Wolfson Light Microscopy Facility, University of Sheffield.
Image 3: Cleared spheroid of a co-culture of HCT-116-GFP (colon cancer) / NIH-3T3-RFP (fibroblasts) cells stained with Hoechst for nuclei. Imaged in an InSphero Akura plate. Dataset was segmented using arivis Pro. Sample courtesy of InSphero AG. Schlieren, Switzerland.
Image 4: Calcium signaling in the zebrafish brain. Courtesy of Anton Nikolaev, University of Sheffield, UK. Data acquired at Wolfson Light Microscopy Facility in the School of Biosciences at the University of Sheffield.
Let's Start the Conversation
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Let's find the information you need. Get in contact with us and request:
See Applications from Early Users
In this Wiley webinar, see applications and hear about experiences from early users of Lightfield 4D-
Keeping Pace with the Pulse of Life
In this webinar, see how to track dynamic processes at high speed in complete sample volumes:
- 3D nuclear tracking in the beating heart of a zebrafish embryo
- Real-time observation of blood cells flowing through the developing zebrafish tail vasculature
- Dynamic imaging of arising cell populations in the developing Drosophila midgut
FAQs
Find answers to frequently asked questions here-
Light field microscopy captures volumetric data of living specimens without the need for focus or sample movement. It enables instant volumetric imaging in a single shot and avoids any time delay within an imaged volume. This enables the imaging of dynamic processes in/of large volumes with extremely high temporal resolution while minimizing the exposure of the living sample to light.
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Its ability to provide volumetric data at high speed while preventing delicate samples from phototoxic effects makes ZEISS Lightfield 4D a powerful tool for studying the dynamics of complex biological systems in 3D over time:
- Neuroscience: real-time imaging of neural activity in 3D across large volumes, such as tracking calcium signals in neurons within living brains
- Developmental Biology: observation of fast cellular processes in organs and entire organisms, like blood flow and heartbeat; as well as gentle long-term imaging of developmental processes, including cell migration
- Cancer Research: High-throughput multi-position 3D imaging of organoids and spheroids
- Plant Science: imaging and tracking of cellular and protein movement within large volumes
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Imaging with Lightfield 4D on a ZEISS LSM is as simple as widefield imaging and takes place in the familiar ZEN environment. The specific challenge for microscope users to orient themselves in the three-dimensionality of their sample is addressed with the Volume Definition representation, a highly intuitive user interface that allows to quickly and reliably define the volume to be imaged.
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Yes, Lightfield 4D detection uses a camera.
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Lightfield 4D uses standard widefield fluorescence excitation with ZEISS solid state light sources.
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Yes, you can acquire multiple color channels in a single experiment. Dual-color imaging is possible with up to 40 volumes per second. The excitation range is 385-740 nm.
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The maximal resolution is 2.2 x 2.2 x 2.8 µm³ (x,y,z) and can be achieved with a 40x objective. Further information can be found in the Lightfield 4D brochure or in the product information brochures of LSM 990 and LSM 910.
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The image processing time per volume depends on the objective lens used and therefore on the volume size and the processing settings. In most cases, it takes only 40-60 seconds to process 100 volumes.
Do you have further questions?
Get in contact with a ZEISS imaging specialist via the form below, we look forward to your message!