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İleri Düzey
Konfokal Mikroskop Uygulama Eğitimi - Sertifika Programı- 00 years
- 00 months
- 00 days
- 00 hours
- 00 minutes
- 00 seconds
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Ankara Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı öğretim üyesidir. 1987 yılından bu yana fotomikrografi ile profesyonel olarak ilgilenmekte olup 1995 yılında ABD’de en iyi konfokal videosu alanında yapılan yarışmada birincilik ödülü aldıktan sonra 1996 yılında Türkiye’deki ilk konfokal sistemini kurmuştur (Zeiss LSM510 meta). Bu sistem ile 20 yıl çalıştıktan ve çok sayıda makale, proje tamamlayıp uzman yetiştirdikten sonra 2018 yılında Zeiss LSM 880 Airyscan sistemine geçiş yapmıştır. Yurt içinde ve yurt dışında mikroskop eğitimleri vermekte, mikrograf yarışmalarında jüri üyeliği yapmaktadır. Konuyla ilgili iki kitabu bulunmaktadır (Elektron Mikroskopide Fotoğraf Teknikleri – 1991; Yaşam Bilimlerinde A’dan Z’ye MİKROSKOPİ-2018)
Program
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14:00 - 15:30
Temel flöresan prensibi
Uyarım ve yayılım filtreleri
Sinyal çaprazlaşması (cross-talk)
Konfokal prensibi
Optik kesit tanımı ve avantajları
Airy Scan® dedektörü
Laser türleri15:45 - 17:00
Konfokal için Numune Hazırlama
Görüntü dosyası formatları (czi, tiff, avi)
Notlar:
Eğitimler sırasında Prof. Alp Can tarafından sağlanacak olan farklı numuneler üzerinde çalışma yapılacaktır.
Cumartesi günü yapılacak kursa katılacak kişi sayısı minimum 6, maksimum 10 kişi olacaktır.
Eğitimin sonunda ZEISS ile ortak imzalı bir katılım sertifikası verilecektir.
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09:30 - 12:30
ZEISS LSM 880/900 eşliğinde ZEN Black Edition Uygulama Seansları
Görüntü Alma Öncesi Ayarlar
Workspace ve Workspace Zoom
Laser Penceresi
Experiment Manager ve Smart Setup
Acquisition Seçenekleri
Imaging setup Penceresi
Acquisition Mode Penceresi
Channels Penceresi
Stage Penceresi
Z-Stack Penceresi
Tile-scan Penceresi
Time series Penceresi
X-Y görüntü alma
X-Y-Z görüntü alma
X-T görüntü alma
T-PMT kullanımı12:30 - 13:30
Ögle Yemegi
13:30 - 17:30
ZEISS LSM 880/900 eşliğinde ZEN Black ver. 14.0 Uygulama Seansları
Görüntü Alma Sonrası Değişkenler
Dosya Görüntüleme Penceresi ve Soldaki Sekmeler
Single channel
Multichannel
Z-stack
Display Penceresi
Dimensions/Player/Graphics Penceresi
File-File Browser
Uzunluk, Alan ölçme
Histogram
Airyscan Dedektörüyle Görüntü Alma
Görüntü Saklama ve Dönüştürme Seçenekleri
File-Export
Dosya saklama formatları
17:30 - 18:00
Soru&Cevap
Notlar:
Eğitimler sırasında Prof. Alp Can tarafından sağlanacak olan farklı numuneler üzerinde çalışma yapılacaktır.
Cumartesi günü yapılacak kursa katılacak kişi sayısı minimum 6, maksimum 10 kişi olacaktır.
Eğitimin sonunda ZEISS ile ortak imzalı bir katılım sertifikası verilecektir.
Kayıt Formu
* Teorik oturumlara katılım ücretsizdir, ancak workshoplara yalnızca doktora öğrencileri kayıt olabilir ve bu oturumlar ücretlidir.
Workshopa katılım için toplamda 40 kişi kabul edilecektir. Kayıt için lütfen belirtilen süre içinde başvurunuzu tamamlayın.
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