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ZEISS Lightfield 4D

ZEISS LSM Lightfield 4D

Instant Volumetric Imaging of Living Organisms and Large 3D Tissues
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One Snap. One Volume.

ZEISS LSM Lightfield 4D expands your confocal with the ability to capture complete volumes – instantly, with a single snap. Capture the fastest movements of an organism at up to 80 volumes per second, or tile your large 3D tissues within a minute.

Additionally, it’s so fast that you don’t need to fix nor aneasthetize your samples.

Capture up to

  • 80 Volumes / Seconds

    and unravel the fastest movements within whole organisms.

Download the ZEISS LSM Lightfield 4D Technology Note

Learn about the technology and its applications
Cover page preview of the white paper 'Comparing Segmentation Methods for Particle Analysis of Lithium Ion Battery Materials using ZEN core'

With this 19-page technology note, learn about:

  • Introduction to imaging technology
  • Applications
  • Technical background
  • Integration into an easy-to-use turn-key system

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If you want to have more information on data processing at ZEISS, please refer to our data privacy and legal notice.

One Snap. One Volume.

Many neuronal and physiological processes occur at very high speeds. Traditional technologies must compromise between volumetric information and image frame rate.

ZEISS LSM Lightfield 4D changes that. You can capture 80 volumes per second for beating zebrafish heart and flashing drosophila neuronal activities. The unique one-snap-one-volume imaging ensures that crucial events are not missed or distorted.

Beating embryonic zebrafish heart at 3 days post fertilization, cardiomyocyte nuclei labelled with mCherry. Courtesy of Stone Elworthy and Emily Noël, School of Biosciences, University of Sheffield, UK

Minimum Light Exposure. Maximum Information Gain.

Phototoxicity is always a challenge for volumetric imaging. The traditional sequential z-stack acquisition exposes the fragile samples to harmful light doses.

ZEISS LSM Lightfield 4D overcomes that. A complete Z-stack is acquired with a single illumination event, reducing light exposure and phototoxic effects to a minimum. You can observe developmental processes, cell migration, vesicle movement or other changes in tissues and organisms that take hours or even days to complete.

Mouse embryo development from 8-cells to blastocyst. Embryos expressing membrane targeted tdTomato and nuclear H2B-GFP. Sample courtesy of Dr. Takafumi Ichikawa from Kyoto University Faculty of Medicine.

Fast Acquisition. Increased Throughput.

Tissue screening and wellplate screening are typically conducted in 2D. Incorporating a 3rd dimension introduces significant technical challenges, including increased duration, the need for advanced focusing strategies, and the management of large volumes of data.

Not with ZEISS LSM Lightfield 4D. The unmatched speed at which ZEISS LSM Lightfield 4D captures multi-color volumes, together with the Direct Processing function, can be used to increase sample throughput and improve experimental statistics.

Epithelial cell layer infected with fungal isolates. Labelled with phalloidin (AF647), anti-fungal (AF488) and Hoechst. Sample Courtesy of Dr Emily Jones, Quadram Institute Bioscience.

Designed for Multi-Modal Workflows

Researchers often need to image both dynamic processes and fine structures – but switching between imaging modalities slows progress and risks sample disturbance.

ZEISS LSM Lightfield 4D removes that. You can capture high-speed volumetric data and detailed morphology using the same ZEISS LSM 990 system. Simply toggle between Lightfield 4D, Airyscan, or confocal modes in ZEN software – no hardware change, no workflow interruption.

Neurons and astrocyte in thick brain sections. Different imaging modalities and data processing combined in one project using the Connect toolkit. Sample courtesy of Luisa Cortes, Microscopy Imaging Center of Coimbra, CNC, University of Coimbra, Portugal.

AI-Assisted Microscopy

The ZEISS Microscopy Copilot®, your personal AI assistant, helps you learn about microscopy and setting up imaging on LSM systems. Access the Microscopy Copilot directly with a click of a button in our imaging software ZEN .Learn how to use the microscope efficiently, get easy access to information relevant for your current work, discover better ways to conduct experiments, and enhance your productivity.

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If you want to have more information on data processing at ZEISS please refer to our data privacy notice.